LOCUS       sequence-316098-        9315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018257
FEATURES             Location/Qualifiers
     source          1..9315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2019..2039
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2040..2063
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2089..2145
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2146..2163)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2146..2162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2155..2177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2371..2390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2490..2512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2550..2568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3354..4553
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4784..5932)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6204..6575)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6608..6717
                     /note="incP origin of transfer"
     rep_origin      6870..7501
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7964..8552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8453..8472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8706..8723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8840..8861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8876..8881,8882..8899,8900..8906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8914..8930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8919..8941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8938..8954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8938..8954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagc
      361 gcttcgccat ggcggcctcg ctcgtgatgc tggccagtac ctacgccaac gcgcagacga
      421 ccgaacagct tgtcaaggga gccacggaca cgtcgaacgt gctgaactat ggcatgggct
      481 acaacctgca gcgcttctcg accctgaacc agatcaataa ggataccgtg aagaatctgg
      541 tccccgtctg gaattactct tttaatgacg accgctccga agagtcgcaa ccgctcgtct
      601 accagggagt gatctacgtg acgagccaca acgcgaccat ggccgtggac gcgaagacgg
      661 gcaagcagat ctggaagagc aagatcgagt acccggcgga gaccccgcgg atcgtgtgtt
      721 gcgggatcat caatcgcggc gccgcgctgt acgacgggaa ggtgttccga acgacgctgg
      781 acgcgaacgt catcgccttg gacgctaagg acggcaagga gctctggcgc cagaaggcgg
      841 cggacatcaa ggagggatac tccatgacgg tggccccgct ggtcgcggac ggcgtggtga
      901 tcacgggcat cagcggtgcc gaattcggga cccgcgggtt catcgacggc tgggaccccg
      961 cgacgggcaa gcacctctgg cgcacacatt cgatcccgag cccggatgaa ccgggcggcg
     1021 atacgtggaa gggcgatacc tggaagctcg gcggcggctc cacctggatc accggttcgt
     1081 atgacccgga gctgaacacg gtgtactggg gcatcgggaa cccgggaccg ttcaactccg
     1141 cggtgcgccc gggcgacaac ctctacacgt gctcggtgct ggccatggac ccgaagacgg
     1201 gaaagatcaa gtggcactac cagttctcgc ccaacaaccc ctttgactat gacagcgtcg
     1261 cggagatggt gcttgcggac atgaacgtcg agggtaagcc gacgaaggtg ctgatggatg
     1321 ccaaccgcaa cggcttcttc tatgtcctgg accgcaccaa cggcaagctg ctggcggcca
     1381 acccgtacgt gaaggtgaac tgggcgaccg gcgtcgacat gaagaccggc cgcccgatcg
     1441 agacagacgt ctcgaaggat gcccgcgagg gcaagaaggt gacggtgtac ccgagcatcc
     1501 tgggcgggaa aaactgggaa ccgatgtcct tcaacccgca gacggggctt gcgtacgcga
     1561 acacgctcgc gttcggcggc aagtataagg cggagccggt caccttcaag cagggcgagt
     1621 ggtacctggg gatggatctg acggatccct gggagttcgg cgacggcgcg cgcggccatc
     1681 tgaaggccat cgaccccatg accggtaagg cgaagtggga ggcgcccgcc gacatcccgc
     1741 ggttctcggg tgtgctgtcc acggccgggg gcgtcgtgtt caccggcgcg ctgaccggag
     1801 aattcgaggc tttcgacgcc gacacgggga aaaagctctg gcagttccag accggctccg
     1861 gcatcgaggg tcagccggtg acctggcagc aggacgacgt gcagtacatc gcggtgacat
     1921 cgggctatgg tggagtgtac tccctcttct cgggcgatga acgcctcgcg aaggtccccc
     1981 cgggcggctc gctgtgggtc ttcgcggtga agcaggccga aaacctctat tttcagggac
     2041 atcatcacca tcaccatcac cactgacagc tatgaccatg attacgccaa gcttgcatgc
     2101 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt
     2161 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2221 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2281 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2341 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2401 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgta tgctcccggc
     2461 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2521 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2581 tgtcatgata ataatggttt cttagcaccc tttctcggtc cttcaacgtt cctgacaacg
     2641 agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc
     2701 ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt
     2761 tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc
     2821 ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc
     2881 gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc
     2941 gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg
     3001 gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga
     3061 ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc
     3121 cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg
     3181 tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac
     3241 tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga
     3301 taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac
     3361 ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc
     3421 tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg
     3481 cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc
     3541 tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg
     3601 ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc tatatcgggc
     3661 ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata
     3721 tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg
     3781 ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt
     3841 tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg
     3901 agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt
     3961 tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc
     4021 aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact
     4081 gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc
     4141 aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg
     4201 gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg
     4261 cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa
     4321 tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc
     4381 tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta
     4441 taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc
     4501 cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga
     4561 aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctaga
     4621 attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc
     4681 gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag
     4741 cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
     4801 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
     4861 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
     4921 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
     4981 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
     5041 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
     5101 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
     5161 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
     5221 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
     5281 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
     5341 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
     5401 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
     5461 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
     5521 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
     5581 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
     5641 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
     5701 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
     5761 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
     5821 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
     5881 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
     5941 cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc
     6001 ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg
     6061 tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagctccg
     6121 cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc
     6181 ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg
     6241 accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca
     6301 tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg
     6361 cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc
     6421 gtgattttgt agccctggcc gacggccagc aggtaggcct acaggctcat gccggccgcc
     6481 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
     6541 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     6601 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     6661 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     6721 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     6781 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     6841 gcagcggaaa agatccgtcg accctttccg acgctcaccg ggctggttgc cctcgccgct
     6901 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     6961 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     7021 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     7081 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     7141 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     7201 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     7261 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     7321 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     7381 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     7441 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     7501 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     7561 aaaaatggca gccaagctga ccacttctgc gctcggccct tccggctggc tggtttattg
     7621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8881 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8941 gaaacagcta tgaccatgat tacgccaagc tagcttcccg cttggtcggg ccgcttcgcg
     9001 agggcccgtt gacgacaacg gtgcgatggg tcccggcccc ggtcaagacg atgccaatac
     9061 gttgcgacac tacgccttgg cacttttaga attgccttat cgtcctgata agaaatgtcc
     9121 gaccagctaa agacatcgcg tccaatcaaa gcctagaaaa tataggcgaa gggacgctaa
     9181 taagtctttc ataagaccgc gcaaatctaa aaatatcctt agattcacga tgcggcactt
     9241 cggatgactt ccgagcgagc ctggaacctc agaaaaacgt ctgagagata ccgcggatct
     9301 cacacaggaa atttc
//
