LOCUS       sequence-316099-       14085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RUBY under the control of At2S3 promoter/marker for Arabidopsis 
            transgenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   He Y, Zhang T, Sun H, Zhan H, Zhao Y
  TITLE     A reporter for noninvasively monitoring gene expression and plant 
            transformation.
  JOURNAL   Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. 
            eCollection 2020.
  PUBMED    33024566
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2020-09-19"; volume: "7:152. doi"; pages: " 
            10.1038/s41438-020-00390-1. eCollection 2020"
COMMENT     Sequence Label: At2S3:RUBY
FEATURES             Location/Qualifiers
     source          1..14085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    178..202
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             652..4602
                     /label=RUBY
     CDS             2152..2208
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3043..3099
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        5306..5485
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5338..5521
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6636..6888
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7463..7480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7463..7479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7472..7494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7701..7725
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8246..9037
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9283..9871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9772..9791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10025..10042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10057..10197
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10123..10142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10283..10305
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10541..10735
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10801..11874)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12303..12932)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
       61 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
      121 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
      181 tacccgccaa tatatcctgt caaacactga tagttttaag ctggcacaac tatatttcca
      241 acatcactag ctaccatcaa aagattgact tctcatctta ctcgattgaa accaaattaa
      301 catagggttt ttatttaaat aaaagtttaa ccttcttttt aaaaaattgt tcatagtgtc
      361 atgtcagaac aagagctaca aatcacacat agcatgcata agcggagcta tgatgagtgg
      421 tattgttttg ttcgtcactt gtcactcttt tccaacacat aatcccgaca acaacgtaag
      481 agcatctctc tctctccaca cacactcatg catgcatgca ttcttacacg tgattgccat
      541 gcaaatctcc tttctcacct ataaatacaa accaaccctt cactacactc ttcactcaaa
      601 ccaaaacaag aaaacataca caaatagcaa aacggtacca acaaaactag tatggatcat
      661 gcgaccctcg ccatgatcct cgcgatctgg ttcatcagct tccacttcat caagctgctg
      721 ttctcccagc agaccaccaa gctgcttccg ccaggaccaa agccgcttcc gatcatcggc
      781 aacatccttg aggtgggcaa gaagccgcat cggtccttcg ccaacctcgc caagattcac
      841 ggcccactca tttccctcag actcggctct gtgaccacca tcgttgtgtc ctctgccgac
      901 gtggccaaag agatgttcct caagaaggat cacccgctct ccaaccgcac gatcccgaat
      961 agtgttacag ccggcgacca ccacaagctc accatgtctt ggctcccggt gtctccgaag
     1021 tggcgcaact tccgcaagat taccgccgtg catctgctct ccccacagag actcgatgcc
     1081 tgccagacat tcaggcacgc caaggtgcag cagctctacg agtacgttca agagtgcgcc
     1141 cagaaaggcc aggccgtgga tattggcaag gccgccttta cgaccagcct caacctcctc
     1201 agcaagctgt tcttcagcgt cgagctggcg caccacaagt cccataccag ccaagagttc
     1261 aaagagctga tctggaacat catggaagat ataggcaagc cgaactacgc cgactacttc
     1321 ccgattctcg gctgcgttga cccatctggc attagaagaa ggctcgcctg ctccttcgac
     1381 aagctgatcg ccgtgttcca gggcatcatc tgcgagagac tcgccccaga ttcctccacc
     1441 acaactacca ccaccaccga cgacgtgctc gatgtgctcc tccagctgtt caagcagaac
     1501 gagctgacga tgggcgagat caaccacctc ctcgtggaca tcttcgacgc cggcaccgat
     1561 accacatcct ccacattcga gtgggtgatg accgagctga tccgcaatcc agagatgatg
     1621 gaaaaggccc aagaggaaat caagcaggtc ctcggcaagg acaagcagat ccaagagtcc
     1681 gacatcatca acctgccgta cctccaggcg atcatcaaag agacactccg cctccatccg
     1741 ccgaccgtgt tcttgctccc aagaaaggcc gacaccgatg tcgagctgta cggctacatc
     1801 gtgccgaagg atgcccagat cctcgtgaac ctctgggcca ttggcaggga cccaaacgcc
     1861 tggcagaacg ccgatatttt cagcccagag cgcttcatcg gctgcgagat cgatgttaag
     1921 ggccgcgatt tcggcctcct tccatttggc gctggccgca gaatttgccc aggcatgaat
     1981 ctcgccatca ggatgctcac cctcatgctc gccacactcc tccagttctt caactggaag
     2041 ctcgaaggcg acatctcccc gaaggacctc gacatggacg agaagttcgg cattgcgctc
     2101 caaaagacca agccgctcaa gctcatcccg attccgcgct acggtagcgg agctaccaat
     2161 tttagcctcc ttaagcaggc aggtgatgta gaagagaacc ccgggcctat gaagatgatg
     2221 aacggcgagg acgccaacga ccagatgatc aaagagtcct tcttcatcac ccacggcaac
     2281 ccgatcctca ccgtcgagga tacacatccg ctcaggccgt tcttcgagac atggcgcgag
     2341 aagattttct ccaagaagcc gaaggccatc ctcatcatct ccggccactg ggagacagtg
     2401 aagccaaccg tgaacgccgt gcacatcaac gacaccatcc acgacttcga cgactaccca
     2461 gccgccatgt accagttcaa gtacccagct ccaggcgagc cagagcttgc gagaaaggtg
     2521 gaagagatcc tcaagaagtc cgggttcgag acagccgaga cagaccaaaa gaggggcctt
     2581 gatcacggcg cctgggttcc actcatgctc atgtatccag aggcggacat cccggtgtgc
     2641 cagctctcag ttcagccaca tctcgacggc acctaccact acaatctcgg cagagccctc
     2701 gcgccgctca agaatgatgg cgtgctcatt attggctccg gcagcgccac acatccactc
     2761 gatgagacac cgcactactt cgatggtgtt gccccttggg ccgctgcctt cgattcttgg
     2821 cttaggaagg ccctcatcaa cggccgcttc gaggaagtga acatctacga gagcaaggcc
     2881 ccgaactgga agctcgccca tccatttcca gagcacttct acccgctcca cgttgtgctc
     2941 ggcgctgctg gtgaaaagtg gaaggccgag ctgatccact cctcctggga tcatggcaca
     3001 ctttgccacg gctcctacaa gttcacctcc gccggatccg gagcaaccaa ctttagcctg
     3061 ctcaagcaag caggagatgt tgaggaaaat cctggcccca tgaccgccat caagatgaac
     3121 accaacggcg agggcgagac acagcacatc ctcatgatcc cgttcatggc gcagggccac
     3181 ctcaggccat ttctcgaact cgccatgttc ctctacaagc gctcccacgt gatcatcacc
     3241 ctgctcacaa ctccgctcaa tgccggcttc ctcaggcacc tccttcacca ccattcctac
     3301 tcctccagcg gcatcaggat cgtcgagctg ccattcaact ccaccaacca cggactccca
     3361 ccgggcatcg agaacaccga taagctcaca ctcccgctcg tggtgtccct cttccattcc
     3421 accatcagcc tcgatccgca cctccgcgat tacatctcca ggcatttcag cccagccagg
     3481 ccaccactct gcgtgatcca tgatgtgttc ctcggctggg ttgaccaggt ggccaaggat
     3541 gtgggctcta caggcgtggt gttcacaaca ggcggcgctt atggcacatc cgcctacgtg
     3601 tccatctgga acgatctccc gcaccagaac tactccgacg accaagagtt cccgctgcca
     3661 ggcttcccag agaaccataa gttccgcagg tcccagctcc atcggttcct cagatatgcc
     3721 gacggctccg acgattggtc caagtatttc cagccgcagc tccgccagtc catgaagtct
     3781 tttggctggc tctgcaactc cgtggaagag atcgagacac tcggcttctc catcctccgc
     3841 aactacacca agctgccgat ctggggcatc ggcccactta ttgcttcccc agtgcagcac
     3901 tcctcctccg acaacaattc aacaggcgcc gagttcgtgc agtggctcag cctcaaagag
     3961 ccggactccg tcctctacat ctccttcggc tcccagaaca cgatcagccc gacgcagatg
     4021 atggaactcg ctgctggcct tgagtcctcc gagaagccat tcctctgggt gatcagagcc
     4081 ccgttcggct tcgacatcaa cgaagagatg cgcccagagt ggctgccaga gggctttgag
     4141 gaacgcatga aggtgaagaa acagggcaag ctcgtgtaca agctcggccc gcagcttgag
     4201 atcctcaacc atgaatccat cggcggcttt ctcacccact gcggatggaa cagcatcctt
     4261 gagtctcttc gcgagggcgt tccgatgctt ggatggccac ttgctgccga gcaggcctac
     4321 aacctcaagt acctcgaaga tgagatgggc gtcgcggttg agcttgctag aggcctcgaa
     4381 ggcgagatct ccaaagagaa ggtcaagcgc atcgtcgaga tgatccttga gcgcaacgag
     4441 ggctccaaag gctgggagat gaagaatcgc gccgtggaaa tgggcaaaaa gctcaaggac
     4501 gccgtgaacg aggaaaaaga gctgaagggc tcctccgtga aggcgatcga cgatttcctc
     4561 gacgccgtca tgcaggccaa acttgagcca agcctccagt gatagtgaat atgaagatga
     4621 agatgaaata tttggtgtgt caaataaaaa ggttgtgtgc ttaagtttgt gtttttttct
     4681 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aacatctcat
     4741 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctc
     4801 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagac
     4861 ctaactgagt aagctagctt gttcgagtat tatggcattg ggaaaactgt ttttcttgta
     4921 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
     4981 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
     5041 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
     5101 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
     5161 atgaggagta aaacactaga tccccaaaca agcttgaaac tgaaggcggg aaacgacaat
     5221 ctgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca
     5281 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg
     5341 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
     5401 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
     5461 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc
     5521 accggatccg ctagaggatc tcgacctgca agatcccggg gggcaatgag atatgaaaaa
     5581 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     5641 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atataggagg
     5701 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     5761 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     5821 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     5881 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     5941 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     6001 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     6061 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     6121 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     6181 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     6241 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     6301 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     6361 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     6421 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     6481 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     6541 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgggatctt ggaggtgatg
     6601 taacatgatc acaagctgat cccccgaatt tccccgatcg ttcaaacatt tggcaataaa
     6661 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     6721 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     6781 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     6841 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
     6901 cccctcgaca gcttgcatgc cagcttgggc tgcaggtcga cgcgtcaatt gatatcgatc
     6961 caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
     7021 tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg cattggaaaa
     7081 gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca gtttttgttt
     7141 tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt ccttttgttc
     7201 attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta agtaagaaag
     7261 gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct ttcacctctc
     7321 tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa gtttactatg
     7381 agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttt ttgtgtcgag
     7441 ggggtaccga gtcgaggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgggtacc
     7621 gagctcgaat tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     7681 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     7741 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     7801 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     7861 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     7921 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     7981 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     8041 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     8101 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     8161 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     8221 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     8281 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     8341 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     8401 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     8461 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     8521 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     8581 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     8641 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     8701 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     8761 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     8821 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     8881 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     8941 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     9001 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     9061 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     9121 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     9181 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     9241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10021 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
    10081 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
    10141 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
    10201 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    10261 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
    10321 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
    10381 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
    10441 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
    10501 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
    10561 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
    10621 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
    10681 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
    10741 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
    10801 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
    10861 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
    10921 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
    10981 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
    11041 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
    11101 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
    11161 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
    11221 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
    11281 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
    11341 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
    11401 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
    11461 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
    11521 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
    11581 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
    11641 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
    11701 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
    11761 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
    11821 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
    11881 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
    11941 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
    12001 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
    12061 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
    12121 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
    12181 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
    12241 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
    12301 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
    12361 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
    12421 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
    12481 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    12541 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    12601 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    12661 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    12721 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    12781 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    12841 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    12901 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    12961 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    13021 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    13081 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    13141 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    13201 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    13261 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    13321 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    13381 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    13441 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    13501 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    13561 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    13621 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    13681 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    13741 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    13801 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    13861 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    13921 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    13981 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    14041 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgc
//
