LOCUS       sequence-31610-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPN8
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gttccgcgtg gtagtaacgc tttgaagtac ttgggccagg
      121 atttcaagac cctgaggcaa cagtgcttgg actcaggggt cctatttaag gaccctgagt
      181 tcccagcatg tccatcagct ttgggctaca aggatcttgg accaggctct ccgcaaactc
      241 aaggcatcat ctggaagcgg cccacggagt tgtgtcccag ccctcagttt atcgttggtg
      301 gagccacgcg cacagacatt tgtcagggtg gtctaggtga ctgctggctt ctggctgcca
      361 ttgcctccct gaccctgaat gaagagctgc tttaccgggt ggtccccagg gaccaggact
      421 tccaggagaa ctatgcggga atctttcact ttcagttctg gcagtacgga gagtgggtgg
      481 aggtggtcat tgacgacagg ctgcccacca agaatggaca gctgctcttc ctacactcgg
      541 aacaaggcaa tgaattctgg agtgccctgc tggagaaagc ctatgccaag cttaatggtt
      601 gttatgaggc tctcgctgga ggttccacag tggaggggtt tgaggatttc acaggtggca
      661 tctctgagtt ttatgacctg aagaaaccac cagccaatct atatcagatc atccggaagg
      721 ccctctgtgc ggggtctctg ctgggctgct ccattgatgt ctacagtgca gccgaagccg
      781 aagccatcac cagccagaag ctggttaaga gtcatgcgta ctctgtcact ggagtcgaag
      841 aggtgaattt ccagggccat ccagagaagc tgatcagact caggaatcca tggggtga
//
