LOCUS       sequence-316100-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Son EY, Ichida JK, Wainger BJ, Toma JS, Rafuse VF, Woolf CJ, Eggan K
  TITLE     Conversion of mouse and human fibroblasts into functional spinal 
            motor neurons.
  JOURNAL   Cell Stem Cell. 2011 Sep 2;9(3):205-18.
  PUBMED    21852222
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2011-09-2"; volume: "9(3)"; pages: "205-18"
COMMENT     Sequence Label: pMXs-Pax6
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1915..1939
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3252..3276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3447..3916
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3986..4003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4157..4745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4237..4256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4909..5700,5701..5769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5532..5551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5770..5874)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aatcacaagt
     1921 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tgcagaacag tcacagcgga
     1981 gtgaatcagc ttggtggtgt ctttgtcaac gggcggccac tgccggactc cacccggcag
     2041 aagatcgtag agctagctca cagcggggcc cggccgtgcg acatttcccg aattctgcag
     2101 gtatccaacg gttgtgtgag taaaattctg ggcaggtatt acgagactgg ctccatcaga
     2161 cccagggcaa tcggagggag taagccaaga gtggcgactc cagaagttgt aagcaaaata
     2221 gcccagtata aacgggagtg cccttccatc tttgcttggg aaatccgaga cagattatta
     2281 tccgaggggg tctgtaccaa cgataacata cccagtgtgt catcaataaa cagagttctt
     2341 cgcaacctgg ctagcgaaaa gcaacagatg ggcgcagacg gcatgtatga taaactaagg
     2401 atgttgaacg ggcagaccgg aagctggggc acacgccctg gttggtatcc cgggacttca
     2461 gtaccagggc aacccacgca agatggctgc cagcaacagg aaggaggggg agagaacacc
     2521 aactccatca gttctaacgg agaagactcg gatgaagctc agatgcgact tcagctgaag
     2581 cggaagctgc aaagaaatag aacatctttt acccaagagc agattgaggc tctggagaaa
     2641 gagtttgaga ggacccatta tccagatgtg tttgcccggg aaagactagc agccaaaata
     2701 gatctacctg aagcaagaat acaggtatgg ttttctaatc gaagggccaa atggagaaga
     2761 gaagagaaac tgaggaacca gagaagacag gccagcaaca ctcctagtca cattcctatc
     2821 agcagcagct tcagtaccag tgtctaccag ccaatcccac agcccaccac acctgtctcc
     2881 tccttcacat caggttccat gttgggccga acagacaccg ccctcaccaa cacgtacagt
     2941 gctttgccac ccatgcccag cttcaccatg gcaaacaacc tgcctatgca acccccagtc
     3001 cccagtcaga cctcctcata ctcgtgcatg ctgcccacca gcccgtcagt gaatgggcgg
     3061 agttatgata cctacacccc tccgcacatg caaacacaca tgaacagtca gcccatgggc
     3121 acctcgggga ccacttcaac aggactcatt tcacctggag tgtcagttcc cgtccaagtt
     3181 cccgggagtg aacctgacat gtctcagtac tggcctcgat tacagtaagg cggccaaggg
     3241 tgggcgcgcc gacccagctt tcttgtacaa agtggtgatg acgataaaat aaaagatttt
     3301 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     3361 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     3421 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     3481 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     3541 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     3601 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     3661 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     3721 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     3781 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     3841 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     3901 cagcgggggt ctttcacatg cagcatgtat caaaattmca wcgcgcgggg agaggcggtt
     3961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     4861 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5881 tc
//
