LOCUS       sequence-316104-       16573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RUBY under the control of YUC4 promoter/tissue specific marker for 
            gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   He Y, Zhang T, Sun H, Zhan H, Zhao Y
  TITLE     A reporter for noninvasively monitoring gene expression and plant 
            transformation.
  JOURNAL   Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. 
            eCollection 2020.
  PUBMED    33024566
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2020-09-19"; volume: "7:152. doi"; pages: " 
            10.1038/s41438-020-00390-1. eCollection 2020"
COMMENT     Sequence Label: YUC4:RUBY
FEATURES             Location/Qualifiers
     source          1..16573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..36
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2974..6924
                     /label=RUBY
     CDS             4474..4530
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5365..5421
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        7628..7807
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7660..7843
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8958..9210
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9785..9802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9785..9801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9794..9816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10023..10047
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10568..11359
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11605..12193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12094..12113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12347..12364
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12379..12519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12445..12464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12605..12627
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12863..13057
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13123..14196)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14625..15254)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt atgtccaaca
       61 tgcatgcgtc tatatatggt atatatgagt gtggtcatgt aactatggtg agaaaaattg
      121 aggttgaggg caaattcaag cctgacagaa aagttccaaa gagcattgag tcggataatg
      181 cagaaggaga aattggaaga agaggccaga gcaagatgac gttttcatgg gaccctaaat
      241 tcttttgtct acattgttat attcatattt ttgccattat cgatagtgta aacaagattg
      301 ctgatggtga tccatccaag gaccacaata ttcctcgtct aaccaaaggg accatcaata
      361 cactatgtaa catgcatata gagtagatta atagtatagc cgatacactt gtataacttg
      421 tatttttctt ccagcttagt catgagtcta ggctctatta aactgaattt taggaccatt
      481 tgaattaaca aaagaaaatg tatggaccat tctattatac caagcatgca taaataatga
      541 gtctatagaa tcgtttgatt tcgaagttat ctttacgtaa gaaacatttg attctcgtcc
      601 aagctaagta agtttatgca cacctgatct ttaaaacggt tatgtagtgc tagttaaaga
      661 aaatttgaac tagattgaat ttttgctatt gaataagtat ctttcgttta cctaattaat
      721 aaaatatgta tatattgaaa ctagctagcg ttttagttta gcacaaattt tgtgtttact
      781 agaactagct agagatacat gcatgcagaa agataattat attagtttca aatatcatat
      841 tccaatatat tgattttctc acgtataatt agaatatttt tttggtttta attagaaatt
      901 atttgcttac tataaggatt gtaaaagaaa ttgaacatgt catgattata tgtaagtgga
      961 gtactaacgt aaataatagg aatgatacat gaaaaaatgg cattttaatt aaatcttgtt
     1021 gataaagtca ttcatctctt gaccataata aggtttttat ttgattggag caaacaaaac
     1081 atttgggtct atgtagtaat aatatgcatc caagtcaagc gtatatacag tttatttttc
     1141 gattaaataa tacccagaaa gcaagttaat atagttaaaa catttacgtt ggatatcgtt
     1201 tacgtcttaa tcaattgtgt ctgtgtgaaa tggtatccat aataaggtca aacgttaatc
     1261 gatgagatga ggtttagcat gcaatgcatc attaaattta cagcttaaac taccatgcgc
     1321 atatatacat ttctgactaa aataagtcac attaaaataa catttaagtc ccaatatttc
     1381 tcttcctctt tacctataaa gtataaacta tatacatata tatacaaaaa tatttcatcc
     1441 ttaggatttt tttagtaatg atatctcgta tttttataat ttttgattct atatgcatat
     1501 tattttaaga tatcatagaa gtaattttgg ttcatatctg ttagtaacta gtttatagaa
     1561 tctattgaac aaaacgaaat atcagctatt taatcagccg tacatttata agtaaatgtg
     1621 atgttgttgc gtccagtata aaatccaatc tagatttaaa tgatccataa ttagttgctt
     1681 tgaagaatat ggcttcgcat aaaatatgtt aaatattgat attgcaacct tatagagatc
     1741 ataggggaag tggaggtcgg ctgcattagt tgacatttaa cgaaggagtg gcaagtcaaa
     1801 ttacattttc cacatatcca cttattaatt atgtgtttgg tgcaattctc attaattgtg
     1861 cttgaatata tcatccctcc agctacctca aaatcaaagc gtagaagtag ttatcttttg
     1921 attatgtatc tttctttctc acgaaaatgt aataatctat atggtatact ataatttatg
     1981 ttggaaagaa gatgaatact tatgatggaa agaaggtcat ctaaaccttt gttaaagtaa
     2041 acaatatttg attaattgat aatttaatga catgaatctt ctgtttccac ttacttgacc
     2101 ccaacaaact ttcggtaaaa gatttaatta taacaattgt acgaatgtga atatgtgaat
     2161 gtgatcatgg aagtcatgaa acacgtcaac gcatatatca tatactacaa tatagttagc
     2221 ggatgttcgt tttagtgttt aaagacatgt ttagctttta catcctgaca tcaacactgt
     2281 tttaatgttt tttgtactaa acatagaact gttttttttt taataaacgt acactataca
     2341 caacataatt gaattatatt taacataatg cactattaac atatgttgct cacgaaagta
     2401 tcaaagtaat taaaatctta accataatat atagtaatca tgttggatcg cattatcgca
     2461 tataaataaa ttcttagtga ttataaaggg aaaaaaatag tcattaattg tagaaagaaa
     2521 tttagtttga ctaatagtaa agaatctatg gcaaagattt ttccagtggt aatatttaat
     2581 atctccacca tttttccctt cttagaccta ctctactccc tacactatac acatcacaat
     2641 acttcttctc ttttctctct ctctcctccc ccattactta ttgagaaaca cacacacaca
     2701 aatcctttcc ttttctctca cgccttctct ctctctctca ctcactctcg agcatttaca
     2761 ccacctcatc ttacgtggtc tctatataat ttccaacttg gtttcttggt ttttcccgag
     2821 aaacaatcaa aagaagaccc taaaacagga cacttcttag gtgcatgtgt gtgtgatcgt
     2881 cagagaaaga taaagacact ttcctcttcc ttccttcttc ttctttctta ctctctctct
     2941 ctctctttcg cttttattag tcgacaaact agtatggatc atgcgaccct cgccatgatc
     3001 ctcgcgatct ggttcatcag cttccacttc atcaagctgc tgttctccca gcagaccacc
     3061 aagctgcttc cgccaggacc aaagccgctt ccgatcatcg gcaacatcct tgaggtgggc
     3121 aagaagccgc atcggtcctt cgccaacctc gccaagattc acggcccact catttccctc
     3181 agactcggct ctgtgaccac catcgttgtg tcctctgccg acgtggccaa agagatgttc
     3241 ctcaagaagg atcacccgct ctccaaccgc acgatcccga atagtgttac agccggcgac
     3301 caccacaagc tcaccatgtc ttggctcccg gtgtctccga agtggcgcaa cttccgcaag
     3361 attaccgccg tgcatctgct ctccccacag agactcgatg cctgccagac attcaggcac
     3421 gccaaggtgc agcagctcta cgagtacgtt caagagtgcg cccagaaagg ccaggccgtg
     3481 gatattggca aggccgcctt tacgaccagc ctcaacctcc tcagcaagct gttcttcagc
     3541 gtcgagctgg cgcaccacaa gtcccatacc agccaagagt tcaaagagct gatctggaac
     3601 atcatggaag atataggcaa gccgaactac gccgactact tcccgattct cggctgcgtt
     3661 gacccatctg gcattagaag aaggctcgcc tgctccttcg acaagctgat cgccgtgttc
     3721 cagggcatca tctgcgagag actcgcccca gattcctcca ccacaactac caccaccacc
     3781 gacgacgtgc tcgatgtgct cctccagctg ttcaagcaga acgagctgac gatgggcgag
     3841 atcaaccacc tcctcgtgga catcttcgac gccggcaccg ataccacatc ctccacattc
     3901 gagtgggtga tgaccgagct gatccgcaat ccagagatga tggaaaaggc ccaagaggaa
     3961 atcaagcagg tcctcggcaa ggacaagcag atccaagagt ccgacatcat caacctgccg
     4021 tacctccagg cgatcatcaa agagacactc cgcctccatc cgccgaccgt gttcttgctc
     4081 ccaagaaagg ccgacaccga tgtcgagctg tacggctaca tcgtgccgaa ggatgcccag
     4141 atcctcgtga acctctgggc cattggcagg gacccaaacg cctggcagaa cgccgatatt
     4201 ttcagcccag agcgcttcat cggctgcgag atcgatgtta agggccgcga tttcggcctc
     4261 cttccatttg gcgctggccg cagaatttgc ccaggcatga atctcgccat caggatgctc
     4321 accctcatgc tcgccacact cctccagttc ttcaactgga agctcgaagg cgacatctcc
     4381 ccgaaggacc tcgacatgga cgagaagttc ggcattgcgc tccaaaagac caagccgctc
     4441 aagctcatcc cgattccgcg ctacggtagc ggagctacca attttagcct ccttaagcag
     4501 gcaggtgatg tagaagagaa ccccgggcct atgaagatga tgaacggcga ggacgccaac
     4561 gaccagatga tcaaagagtc cttcttcatc acccacggca acccgatcct caccgtcgag
     4621 gatacacatc cgctcaggcc gttcttcgag acatggcgcg agaagatttt ctccaagaag
     4681 ccgaaggcca tcctcatcat ctccggccac tgggagacag tgaagccaac cgtgaacgcc
     4741 gtgcacatca acgacaccat ccacgacttc gacgactacc cagccgccat gtaccagttc
     4801 aagtacccag ctccaggcga gccagagctt gcgagaaagg tggaagagat cctcaagaag
     4861 tccgggttcg agacagccga gacagaccaa aagaggggcc ttgatcacgg cgcctgggtt
     4921 ccactcatgc tcatgtatcc agaggcggac atcccggtgt gccagctctc agttcagcca
     4981 catctcgacg gcacctacca ctacaatctc ggcagagccc tcgcgccgct caagaatgat
     5041 ggcgtgctca ttattggctc cggcagcgcc acacatccac tcgatgagac accgcactac
     5101 ttcgatggtg ttgccccttg ggccgctgcc ttcgattctt ggcttaggaa ggccctcatc
     5161 aacggccgct tcgaggaagt gaacatctac gagagcaagg ccccgaactg gaagctcgcc
     5221 catccatttc cagagcactt ctacccgctc cacgttgtgc tcggcgctgc tggtgaaaag
     5281 tggaaggccg agctgatcca ctcctcctgg gatcatggca cactttgcca cggctcctac
     5341 aagttcacct ccgccggatc cggagcaacc aactttagcc tgctcaagca agcaggagat
     5401 gttgaggaaa atcctggccc catgaccgcc atcaagatga acaccaacgg cgagggcgag
     5461 acacagcaca tcctcatgat cccgttcatg gcgcagggcc acctcaggcc atttctcgaa
     5521 ctcgccatgt tcctctacaa gcgctcccac gtgatcatca ccctgctcac aactccgctc
     5581 aatgccggct tcctcaggca cctccttcac caccattcct actcctccag cggcatcagg
     5641 atcgtcgagc tgccattcaa ctccaccaac cacggactcc caccgggcat cgagaacacc
     5701 gataagctca cactcccgct cgtggtgtcc ctcttccatt ccaccatcag cctcgatccg
     5761 cacctccgcg attacatctc caggcatttc agcccagcca ggccaccact ctgcgtgatc
     5821 catgatgtgt tcctcggctg ggttgaccag gtggccaagg atgtgggctc tacaggcgtg
     5881 gtgttcacaa caggcggcgc ttatggcaca tccgcctacg tgtccatctg gaacgatctc
     5941 ccgcaccaga actactccga cgaccaagag ttcccgctgc caggcttccc agagaaccat
     6001 aagttccgca ggtcccagct ccatcggttc ctcagatatg ccgacggctc cgacgattgg
     6061 tccaagtatt tccagccgca gctccgccag tccatgaagt cttttggctg gctctgcaac
     6121 tccgtggaag agatcgagac actcggcttc tccatcctcc gcaactacac caagctgccg
     6181 atctggggca tcggcccact tattgcttcc ccagtgcagc actcctcctc cgacaacaat
     6241 tcaacaggcg ccgagttcgt gcagtggctc agcctcaaag agccggactc cgtcctctac
     6301 atctccttcg gctcccagaa cacgatcagc ccgacgcaga tgatggaact cgctgctggc
     6361 cttgagtcct ccgagaagcc attcctctgg gtgatcagag ccccgttcgg cttcgacatc
     6421 aacgaagaga tgcgcccaga gtggctgcca gagggctttg aggaacgcat gaaggtgaag
     6481 aaacagggca agctcgtgta caagctcggc ccgcagcttg agatcctcaa ccatgaatcc
     6541 atcggcggct ttctcaccca ctgcggatgg aacagcatcc ttgagtctct tcgcgagggc
     6601 gttccgatgc ttggatggcc acttgctgcc gagcaggcct acaacctcaa gtacctcgaa
     6661 gatgagatgg gcgtcgcggt tgagcttgct agaggcctcg aaggcgagat ctccaaagag
     6721 aaggtcaagc gcatcgtcga gatgatcctt gagcgcaacg agggctccaa aggctgggag
     6781 atgaagaatc gcgccgtgga aatgggcaaa aagctcaagg acgccgtgaa cgaggaaaaa
     6841 gagctgaagg gctcctccgt gaaggcgatc gacgatttcc tcgacgccgt catgcaggcc
     6901 aaacttgagc caagcctcca gtgatagtga atatgaagat gaagatgaaa tatttggtgt
     6961 gtcaaataaa aaggttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     7021 atttgtggct ttttctaata ttaaatgaat gtaacatctc attataatga ataaacaaat
     7081 gtttctataa tccattgtga atgttttgtt ggatctcttc tccagcatat aactactgta
     7141 tgtgctatgg tatggactat ggaatatgat taaagataag acctaactga gtaagctagc
     7201 ttgttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     7261 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     7321 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     7381 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     7441 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacacta
     7501 gatccccaaa caagcttgaa actgaaggcg ggaaacgaca atctgatcat gagcggagaa
     7561 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga
     7621 actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga gtttaatgag
     7681 ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca
     7741 gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat
     7801 ccaattagag tctcatattc actctcaatc caaataatct gcaccggatc cgctagagga
     7861 tctcgacctg caagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     7921 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     7981 ggagggcgaa gaatctcgtg ctttcagctt cgatatagga gggcgtggat atgtcctgcg
     8041 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     8101 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     8161 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     8221 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     8281 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     8341 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     8401 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     8461 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     8521 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     8581 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     8641 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     8701 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     8761 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     8821 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     8881 aaaccgacgc cccagcactc gtccgggatc ttggaggtga tgtaacatga tcacaagctg
     8941 atcccccgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     9001 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     9061 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     9121 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     9181 cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga cagcttgcat
     9241 gccagcttgg gctgcaggtc gacgcgtcaa ttgatatcga tccaggcctc ccagctttcg
     9301 tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcccaca
     9361 caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt cttctatcat
     9421 ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa
     9481 atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta
     9541 tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat
     9601 taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat
     9661 tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat
     9721 tatgcaatca agttaagtac agtataggct ttttgtgtcg agggggtacc gagtcgagga
     9781 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     9841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     9901 atcgcccttc ccaacagttg cgcagcctga atggcgggta ccgagctcga attcaattcg
     9961 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
    10021 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
    10081 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
    10141 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
    10201 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
    10261 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc
    10321 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
    10381 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
    10441 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
    10501 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
    10561 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
    10621 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
    10681 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
    10741 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
    10801 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
    10861 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
    10921 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
    10981 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
    11041 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
    11101 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
    11161 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
    11221 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
    11281 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
    11341 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
    11401 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
    11461 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
    11521 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    11641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    11761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    11821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    11881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    11941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    12001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    12061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    12121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    12181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    12241 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    12301 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    12361 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    12421 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    12481 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    12541 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    12601 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    12661 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
    12721 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
    12781 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
    12841 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
    12901 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
    12961 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
    13021 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
    13081 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
    13141 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
    13201 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
    13261 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
    13321 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
    13381 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
    13441 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
    13501 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
    13561 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
    13621 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
    13681 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
    13741 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
    13801 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
    13861 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
    13921 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
    13981 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
    14041 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    14101 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    14161 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    14221 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
    14281 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    14341 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    14401 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    14461 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    14521 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    14581 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    14641 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    14701 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    14761 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    14821 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    14881 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    14941 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    15001 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    15061 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    15121 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    15181 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    15241 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    15301 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    15361 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    15421 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    15481 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    15541 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    15601 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    15661 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    15721 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    15781 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    15841 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    15901 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    15961 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    16021 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    16081 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    16141 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    16201 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    16261 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    16321 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    16381 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    16441 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca
    16501 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
    16561 ctaataaacg ctc
//
