LOCUS       sequence-316106-        9547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Golgi marker ManII-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: pEF-ManII-GFP
FEATURES             Location/Qualifiers
     source          1..9547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..79)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        64..393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      244..379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     306..325
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             419..1213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(473..492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1083..1102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1387..1508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1424..1443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1478..1497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1541..1558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1712..2300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1792..1811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2471..3262,3263..3331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3094..3113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3332..3436)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3512..4674
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3743..4665
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4622..4642
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8162..8869
                     /codon_start=1
                     /product="photostable monomeric superfolder GFP (Valbuena 
                     et al., 2020)"
                     /label=msGFP2
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITGGSGGS"
     primer_bind     complement(8204..8225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8812..8833
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8893..8910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8899..9123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9169..9547,1..50)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9256..9275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
       61 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      121 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
      181 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
      241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgcccctagg ctgactaatt
      301 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
      361 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccccccggga ggtccacaat
      421 ggttgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgcccgacgg
      961 cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     1261 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1321 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1381 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1441 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1501 gtatcttatc atgtctgtat accgtcgatc tttccgcttc ctcgctcact gactcgctgc
     1561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtatgcac gaaccccccg
     1981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3181 tgttgagatc cagttcgatg taacccactc gggcacccaa ctgatcttca gcatctttta
     3241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaga tcgacggatc
     3481 gggagatcgg taccgaattc aagcttcgtg aggctccggt gcccgtcagt gggcagagcg
     3541 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     3601 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3661 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3721 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     3781 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctccagt acgtgattct
     3841 tgatcccgag ctggagccag gggcgggcct tgcgctttag gagccccttc gcctcgtgct
     3901 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3961 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     4021 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aggatctgca cactggtatt
     4081 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     4141 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     4201 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     4261 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctccaggg
     4321 ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     4381 aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     4441 tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg ttggggggag
     4501 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     4561 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4621 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaacacgt
     4681 ggccaccgcc accatgaagt taagtcgcca gttcaccgtg tttggcagcg cgatcttctg
     4741 cgtcgtaatc ttctcactct acctgatgct ggacaggggt cacttggact accctcgggg
     4801 cccgcgccag gagggctcct ttccgcaggg ccagctttca atattgcaag aaaagattga
     4861 ccatttggag cgtttgctcg ctgagaacaa cgagatcatc tcaaatatca gagactcagt
     4921 catcaacctg agcgagtctg tggaggacgg cccgcggggg tcaccaggca acgccagcca
     4981 aggctccatc cacctccact cgccacagtt ggccctgcag gctgacccca gagactgttt
     5041 gtttgcttca cagagtggga gtcagccccg ggatgtgcag atgttggatg tttacgatct
     5101 gattcctttt gataatccag atggtggagt ttggaagcaa ggatttgaca ttaagtatga
     5161 agcggatgag tgggaccatg agcccctgca agtgtttgtg gtgcctcact cccataatga
     5221 cccaggttgg ttgaagactt tcaatgacta ctttagagac aagactcagt atatttttaa
     5281 taacatggtc ctaaagctga aagaagactc aagcaggaag tttatgtggt ctgagatctc
     5341 ttaccttgca aaatggtggg atattataga tattccgaag aaggaagctg ttaaaagttt
     5401 actacagaat ggtcagctgg aaattgtgac cggtggctgg gttatgcctg atgaagccac
     5461 tccacattat tttgccttaa ttgaccaact aattgaaggg caccaatggc tggaaaaaaa
     5521 tctaggagtg aaacctcgat cgggctgggc catagatccc tttggtcatt cacccacaat
     5581 ggcttatctt ctaaagcgtg ctggattttc acacatgctc atccagagag tccattatgc
     5641 aatcaaaaaa cacttctctt tgcataaaac gctggagttt ttctggagac agaattggga
     5701 tcttggatct gctacagaca ttttgtgcca tatgatgccc ttctacagct acgacatccc
     5761 tcacacctgt gggcctgatc ctaaaatatg ctgccagttt gattttaaac ggcttcctgg
     5821 aggcagatat ggttgtccct ggggagttcc cccagaagca atatctcctg gaaatgtcca
     5881 aagcagggct cagatgctat tggatcagta ccggaaaaag tcaaaacttt tccgcactaa
     5941 agttctgctg gctccactgg gagacgactt tcggttcagt gaatacacag agtgggatct
     6001 gcagtgcagg aactacgagc aactgttcag ttacatgaac tcgcagcctc atctgaaagt
     6061 gaagatccag tttggaacct tgtcagatta tttcgacgca ttggagaaag cggtggcagc
     6121 cgagaagaag agtagccagt ctgtgttccc tgccctgagt ggagacttct tcacgtacgc
     6181 tgacagagac gaccattact ggagtggcta cttcacgtcc agacctttct acaaacgaat
     6241 ggacagaata atggaatctc gtataagggc tgctgaaatt ctttaccagt tggccttgaa
     6301 acaagctcag aaatacaaga taaataaatt tctttcatca cctcattaca caacactgac
     6361 agaagccaga aggaacttag gactatttca gcatcatgat gccatcacag gaaccgcgaa
     6421 agactgggtg gttgtggact atggtaccag actctttcag tcattaaatt ctttggagaa
     6481 gataattgga gattctgcat ttcttctcat tttaaaggac aaaaagctgt accagtcaga
     6541 tccttccaaa gccttcttag agatggatac gaagcaaagt tcacaagatt ctctgcccca
     6601 aaaaattata atacaactga gcgcacagga gccaaggtac cttgtggtct acaatccctt
     6661 tgaacaagaa cggcattcag tggtgtccat ccgggtaaac tccgccacag tgaaagtgct
     6721 gtctgattcg ggaaaaccgg tggaggttca agtcagtgca gtttggaacg acatgaggac
     6781 aatttcacaa gcagcctatg aggtttcttt tctagctcat ataccaccac tgggactgaa
     6841 agtgtttaag atcttagagt cacaaagttc aagctcacac ttggctgatt atgtcctata
     6901 taataatgat ggactagcag aaaatggaat attccacgtg aagaacatgg tggatgctgg
     6961 agatgccata acaatagaga atcccttcct ggcgatttgg tttgaccgat ctgggctgat
     7021 ggagaaagtg agaaggaaag aagacagtag acagcatgaa ctgaaggtcc agttcctgtg
     7081 gtacggaacc accaacaaaa gggacaagag cggtgcctac ctcttcctgc ctgacgggca
     7141 gggccagcca tatgtttccc taagaccgcc ctttgtcaga gtgacacgtg gaaggatcta
     7201 ctcagatgtg acctgtttcc tcgaacacgt tactcacaaa gtccgcctgt acaacattca
     7261 gggaatagaa ggtcagtcca tggaagtttc taatattgta aacatcagga atgtgcataa
     7321 ccgtgagatt gtaatgagaa tttcatctaa aataaacaac caaaatagat attatactga
     7381 cctaaatgga tatcagattc agcctagaag gaccatgagc aaattgcctc ttcaagccaa
     7441 cgtttacccg atgtgcacaa tggcgtatat ccaggatgct gagcaccggc tcacgctgct
     7501 ctctgctcag tctctaggtg cttccagcat ggcttctggt cagattgaag tcttcatgga
     7561 tcgaaggctc atgcaggatg ataaccgtgg ccttgggcaa ggcgtccatg acaataagat
     7621 tacagctaat ttgtttcgaa tcctcctcga gaagagaagc gctgtgaaca tggaagaaga
     7681 aaagaagagc cctgtcagct acccttccct cctcagccac atgacttcgt ccttcctcaa
     7741 ccatcccttt ctccccatgg tactaagtgg ccagctcccc tcccctgcct ttgagctgct
     7801 gagtgaattt cctctgctgc agtcctctct accttgtgat atccatctgg tcaacctgcg
     7861 gacaatacaa tcaaagatgg gcaaaggcta ttcggatgag gcagccttga tcctccacag
     7921 gaaagggttt gattgccagt tctccagcag aggcatcggg ctaccctgtt ccactactca
     7981 gggaaagatg tcagttctga aacttttcaa caagtttgct gtggagagtc tcgtcccttc
     8041 ctctctgtcc ttgatgcact cccctccaga tgcccagaac atgagtgaag tcagcctgag
     8101 ccccatggag atcagcacgt tccgtatccg cttgcgttgg accggaggaa gcggaggaag
     8161 catggatagc actgagagcc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     8221 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
     8281 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     8341 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     8401 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcacctt
     8461 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     8521 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     8581 gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     8641 catcaaggcc aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     8701 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     8761 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     8821 gctggagttc gtgaccgccg ccgggatcac tggaggcagt ggcggttctt agtctagaag
     8881 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8941 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     9001 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     9061 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatggc ccgggctcta
     9121 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     9181 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9241 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9301 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9361 acctctcccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9421 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9481 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9541 cgatttc
//
