LOCUS       sequence-31611-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBE2J2
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             624..641
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(694..712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      708..755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(879..898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtatg agcagcacca gcagtaagag ggctccgacc
      121 acggcaaccc agaggctgaa gcaggactac cttcgcatta agaaagaccc ggtgccttac
      181 atctgtgccg agcccctccc ttcgaatatt ctcgagtggc actatgtcgt ccgaggccca
      241 gagatgaccc cttatgaagg tggctattat catggaaaac taatttttcc cagagaattt
      301 cctttcaaac ctcccagtat ctatatgatc actcccaacg ggaggtttaa gtgcaacacc
      361 aggctgtgtc tttctatcac ggatttccac ccggacacgt ggaacccggc ctggtctgtc
      421 tccaccatcc tgactgggct cctgagcttc atggtggaga agggccccac cctgggcagt
      481 atagagacgt cggacttcac gaaaagacaa ctggcagtgc agagtttagc atttaatttg
      541 aaagataaag tcttttgtga attatttcct gaagtcgtgg aggagattaa acaatgatga
      601 cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      661 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      721 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      781 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      901 ctttctcg
//
