LOCUS       sequence-316110-       11215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Daniloski Z, Jordan TX, Wessels HH, Hoagland DA, Kasela S, Legut M, 
            Maniatis S, Mimitou EP, Lu L, Geller E, Danziger O, Rosenberg BR, 
            Phatnani H, Smibert P, Lappalainen T, tenOever BR, Sanjana NE
  TITLE     Identification of Required Host Factors for SARS-CoV-2 Infection in 
            Human Cells.
  JOURNAL   Cell. 2020 Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030.
  PUBMED    33147445
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030."
COMMENT     Sequence Label: pLenti-hACE2-hygro
FEATURES             Location/Qualifiers
     source          1..11215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..1278
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          331..1269
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1226..1246
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3740..3796
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3817..4405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3870..3890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4288..4299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4477..4710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4788..4909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4825..4844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4879..4898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4949..5084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5104..5123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5105..5123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5133..5150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5133..5149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5142..5164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5291..5746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5378..5397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5588..5609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5772..5876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5877..5945,5946..6737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6095..6114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6908..7496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7397..7416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7650..7667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7784..7805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7820..7825,7826..7843,7844..7850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7858..7874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7863..7885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7882..7898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7882..7898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7917..7937
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7919..7937
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7965..8191
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8192..8372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8419..8544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9037..9270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9455..9499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9730..9940
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9752..9948
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9799..9934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9970..10989
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    11060..11177
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
       61 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
      121 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
      181 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      241 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
      301 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
      361 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
      421 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
      481 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
      541 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
      601 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
      661 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
      721 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
      781 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
      841 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
      901 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
      961 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     1021 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     1081 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     1141 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     1201 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     1261 ccatttcagg tgtcgtgagg ccaccatgtc aagctcttcc tggctccttc tcagccttgt
     1321 tgctgtaact gctgctcagt ccaccattga ggaacaggcc aagacatttt tggacaagtt
     1381 taaccacgaa gccgaagacc tgttctatca aagttcactt gcttcttgga attataacac
     1441 caatattact gaagagaatg tccaaaacat gaataatgct ggggacaaat ggtctgcctt
     1501 tttaaaggaa cagtccacac ttgcccaaat gtatccacta caagaaattc agaatctcac
     1561 agtcaagctt cagctgcagg ctcttcagca aaatgggtct tcagtgctct cagaagacaa
     1621 gagcaaacgg ttgaacacaa ttctaaatac aatgagcacc atctacagta ctggaaaagt
     1681 ttgtaaccca gataatccac aagaatgctt attacttgaa ccaggtttga atgaaataat
     1741 ggcaaacagt ttagactaca atgagaggct ctgggcttgg gaaagctgga gatctgaggt
     1801 cggcaagcag ctgaggccat tatatgaaga gtatgtggtc ttgaaaaatg agatggcaag
     1861 agcaaatcat tatgaggact atggggatta ttggagagga gactatgaag taaatggggt
     1921 agatggctat gactacagcc gcggccagtt gattgaagat gtggaacata cctttgaaga
     1981 gattaaacca ttatatgaac atcttcatgc ctatgtgagg gcaaagttga tgaatgccta
     2041 tccttcctat atcagtccaa ttggatgcct ccctgctcat ttgcttggtg atatgtgggg
     2101 tagattttgg acaaatctgt actctttgac agttcccttt ggacagaaac caaacataga
     2161 tgttactgat gcaatggtgg accaggcctg ggatgcacag agaatattca aggaggccga
     2221 gaagttcttt gtatctgttg gtcttcctaa tatgactcaa ggattctggg aaaattccat
     2281 gctaacggac ccaggaaatg ttcagaaagc agtctgccat cccacagctt gggacctggg
     2341 gaagggcgac ttcaggatcc ttatgtgcac aaaggtgaca atggacgact tcctgacagc
     2401 tcatcatgag atggggcata tccagtatga tatggcatat gctgcacaac cttttctgct
     2461 aagaaatgga gctaatgaag gattccatga agctgttggg gaaatcatgt cactttctgc
     2521 agccacacct aagcatttaa aatccattgg tcttctgtca cccgattttc aagaagacaa
     2581 tgaaacagaa ataaacttcc tgctcaaaca agcactcacg attgttggga ctctgccatt
     2641 tacttacatg ttagagaagt ggaggtggat ggtctttaaa ggggaaattc ccaaagacca
     2701 gtggatgaaa aagtggtggg agatgaagcg agagatagtt ggggtggtgg aacctgtgcc
     2761 ccatgatgaa acatactgtg accccgcatc tctgttccat gtttctaatg attactcatt
     2821 cattcgatat tacacaagga ccctttacca attccagttt caagaagcac tttgtcaagc
     2881 agctaaacat gaaggccctc tgcacaaatg tgacatctca aactctacag aagctggaca
     2941 gaaactgttc aatatgctga ggcttggaaa atcagaaccc tggaccctag cattggaaaa
     3001 tgttgtagga gcaaagaaca tgaatgtaag gccactgctc aactactttg agcccttatt
     3061 tacctggctg aaagaccaga acaagaattc ttttgtggga tggagtaccg actggagtcc
     3121 atatgcagac caaagcatca aagtgaggat aagcctaaaa tcagctcttg gagataaagc
     3181 atatgaatgg aacgacaatg aaatgtacct gttccgatca tctgttgcat atgctatgag
     3241 gcagtacttt ttaaaagtaa aaaatcagat gattcttttt ggggaggagg atgtgcgagt
     3301 ggctaatttg aaaccaagaa tctcctttaa tttctttgtc actgcaccta aaaatgtgtc
     3361 tgatatcatt cctagaactg aagttgaaaa ggccatcagg atgtcccgga gccgtatcaa
     3421 tgatgctttc cgtctgaatg acaacagcct agagtttctg gggatacagc caacacttgg
     3481 acctcctaac cagccccctg tttccatatg gctgattgtt tttggagttg tgatgggagt
     3541 gatagtggtt ggcattgtca tcctgatctt cactgggatc agagatcgga agaagaaaaa
     3601 taaagcaaga agtggagaaa atccttatgc ctccatcgat attagcaaag gagaaaataa
     3661 tccaggattc caaaacactg atgatgttca gacctccttt ggtaccgaga cctcccaggt
     3721 ggcgcccgct ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     3781 agagaatcct ggaccgtaac cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     3841 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3901 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3961 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4021 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4081 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4201 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4261 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4321 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4381 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     4441 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4501 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4681 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     4741 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4921 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     5101 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     5161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5281 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5701 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5761 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5821 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5881 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5941 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6001 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6061 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7861 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7921 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7981 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     8041 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     8101 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     8161 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     8221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8341 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8401 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8461 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8521 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8581 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8641 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8701 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8761 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8821 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8881 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8941 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     9001 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     9061 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9121 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9181 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9241 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9301 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9361 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9421 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9481 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9541 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9601 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9661 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9721 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     9781 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9841 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9901 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     9961 ggtaccacca tgaaaaagcc tgaactcacc gctacctctg tcgagaagtt tctgatcgaa
    10021 aagttcgaca gcgtctccga cctgatgcag ctctccgagg gcgaagaatc tcgggctttc
    10081 agcttcgatg tgggagggcg tggatatgtc ctgcgggtga atagctgcgc cgatggtttc
    10141 tacaaagatc gctatgttta tcggcacttt gcatccgccg ctctccctat tcccgaagtg
    10201 cttgacattg gggagttcag cgagagcctg acctattgca tctcccgccg tgcacagggt
    10261 gtcaccttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctccagcc cgtcgccgag
    10321 gccatggatg ccatcgctgc cgccgatctt agccagacca gcgggttcgg cccattcgga
    10381 cctcaaggaa tcggtcaata cactacatgg cgcgatttca tctgcgctat tgctgatccc
    10441 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcctccgt cgcccaggct
    10501 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcc
    10561 gatttcggct ccaacaatgt cctgaccgac aatggccgca taacagccgt cattgactgg
    10621 agcgaggcca tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccc
    10681 tggttggctt gtatggagca gcagacccgc tacttcgagc ggaggcatcc cgagcttgca
    10741 ggatctcctc ggctccgggc ttatatgctc cgcattggtc ttgaccaact ctatcagagc
    10801 ttggttgacg gcaatttcga tgatgcagct tgggctcagg gtcgctgcga cgcaatcgtc
    10861 cggtccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc tgccgtctgg
    10921 accgatggct gtgtggaagt gctcgccgat agtggaaaca gacgccccag cactcgtcct
    10981 agggcaaagg atctgcagta atgaggcgcg ccgttaacga attctagatc ttgagacaaa
    11041 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    11101 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    11161 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcg
//
