LOCUS       sequence-316113-        6232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ExbB, ExbD proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Kopp DR, Postle K
  TITLE     The Intrinsically Disordered Region of ExbD Is Required for Signal 
            Transduction.
  JOURNAL   J Bacteriol. 2020 Mar 11;202(7). pii: JB.00687-19. doi: 
            10.1128/JB.00687-19. Print 2020 Mar 11.
  PUBMED    31932309
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2020-03-11"; volume: "202(7). pii: JB.00687-19. 
            doi"; pages: " 10.1128/JB.00687-19. Print 2020 Mar 11"
COMMENT     Sequence Label: pKP1657
FEATURES             Location/Qualifiers
     source          1..6232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3090..3107)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3260..3346
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3438..3465
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3484..3575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3576..3644,3645..4436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3794..3813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4478..4933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4565..4584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4775..4796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5044..5632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5533..5552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5786..5803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5969..5991
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6129..6148
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgattcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc
       61 cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg
      121 agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagctgca aaaattgcgg
      181 cgttaaatcc ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca tcatattgcg
      241 cagttcacga atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa
      301 tccctggcgt aatgcggcag aaaacggggt ggagagcgcc gccagagaca ctttcaaaaa
      361 gctttccgcc agtggcagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg
      421 caaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc
      481 cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc
      541 gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca acggcagcgg
      601 catttcgcca atctcatcga gaaacagcgt acctccgtgg gcgatttcaa acagcccggc
      661 gcgaccgccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc
      721 cagcagcgat tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg actttttgcc
      781 ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt
      841 gcccgtcccc gtttccccct caatcaacac cgctgcactg gagcgggcat acagcaaaat
      901 agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg
      961 agttctcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat
     1021 atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt
     1081 cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc
     1141 gttagctttt agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg
     1201 atcgaggcgc aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata
     1261 agtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc
     1321 gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc
     1381 gttagatcca gcggcgatga tggcgtcaca gcgttcgttt gccagtttct tgtggatgta
     1441 ggtcaccgct ttttcaaagc caagctggat aggggtaatg ttcgccaggt gatcaaactc
     1501 gaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg
     1561 tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa
     1621 tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa
     1681 acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa
     1741 ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg
     1801 caaataacaa gttgataaca agctagcagg aggaattcac catgggtaat aatttaatgc
     1861 agacggacct ttccgtctgg ggtatgtatc agcacgccga tattgtcgtt aagtgcgtga
     1921 tgattgggct tattttggcc tccgtagtca cctgggcaat cttcttcagt aagagcgtag
     1981 agttcttcaa tcagaagcgt cgccttaagc gcgagcagca actgctggct gaagcgcgtt
     2041 ccttaaacca ggccaacgat atcgccgctg attttggtag caaaagctta agcctgcatt
     2101 tgctcaatga agcgcagaat gagctggaac tgtcagaagg cagcgacgat aacgaaggta
     2161 ttaaagaacg taccagcttc cgtctggagc gtcgggtcgc cgcagtgggt cgtcaaatgg
     2221 gccgcggtaa cggctatctg gcaaccatcg gcgcgatttc tccgttcgtt ggtctgtttg
     2281 gtacggtctg gggcatcatg aacagcttta ttggtatcgc gcaaacgcag accactaacc
     2341 tggcagtcgt tgcgccgggt atcgcagaag ctctgttagc aacggcaatc ggcctcgtgg
     2401 cagcgattcc tgcggtcgtt atctataacg tatttgcacg ccagattggc ggctttaaag
     2461 cgatgctggg tgatgttgca gcgcaggtat tgttgctgca aagccgtgac ctggatctgg
     2521 aagccagcgc cgctgcgcat ccggttcgtg tcgcacaaaa attacgcgca ggataatatc
     2581 cgatggcaat gcatcttaac gaaaacctcg acgataacgg cgaaatgcat gatatcaacg
     2641 tgacgccgtt tatcgacgtg atgttggttc tgctgattat ctttatggtg gcggcaccgt
     2701 tagcgacggt agatgtgaag gtgaacttgc ctgcttctac cagcacgccg cagccgcgcc
     2761 cggaaaaacc ggtttatctg tcggtgaagg cagacaactc gatgtttatc ggtaacgatc
     2821 cggtcaccga tgaaacaatg attacggcgt tgaatgcgtt aaccgaaggc aagaaagaca
     2881 ccaccatctt cttccgagcg gacaaaaccg tcgattacga gacgttgatg aaggtaatgg
     2941 atacgctgca tcaggcgggt tacctgaaga taggtctggt cggcgaagaa accgccaaag
     3001 cgaagtaacc atggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
     3061 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     3121 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     3181 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     3241 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3301 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     3361 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     3421 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     3481 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     3841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4441 cagaccaagt ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta
     4501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4681 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4801 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     4861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4921 acgtttacaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4981 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5041 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5101 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5161 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5221 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5281 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5341 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5401 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5461 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5521 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5581 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5641 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5701 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5761 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     5821 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag ggtcatggct
     5881 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5941 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6001 tcatcaccga aacgcgcgag gcagaaggag atggcgccca acagtccccc ggccacgggg
     6061 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     6121 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     6181 ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc
//
