LOCUS       sequence-316117-        5783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ExbB protein; the exbB gene replaces tonB gene in 
            BamHI site of pKP325; includes pBAD promoter and optimized RBS from 
            pBAD24-derived pKP660 (ExbB, ExbD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5783)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP1469
FEATURES             Location/Qualifiers
     source          1..5783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             complement(3731..4390)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4305..4324
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4391..4493)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4885..4902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5019..5564)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5099..5118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc aggaggaatt caccatgggt aataatttaa tgcagacgga cctttccgtc
     1261 tggggtatgt atcagcacgc cgatattgtc gttaagtgcg tgatgattgg gcttattttg
     1321 gcctccgtag tcacctgggc aatcttcttc agtaagagcg tagagttctt caatcagaag
     1381 cgtcgcctta agcgcgagca gcaactgctg gctgaagcgc gttccttaaa ccaggccaac
     1441 gatatcgccg ctgattttgg tagcaaaagc ttaagcctgc atttgctcaa tgaagcgcag
     1501 aatgagctgg aactgtcaga aggcagcgac gataacgaag gtattaaaga acgtaccagc
     1561 ttccgtctgg agcgtcgggt cgccgcagtg ggtcgtcaaa tgggccgcgg taacggctat
     1621 ctggcaacca tcggcgcgat ttctccgttc gttggtctgt ttggtacggt ctggggcatc
     1681 atgaacagct ttattggtat cgcgcaaacg cagaccacta acctggcagt cgttgcgccg
     1741 ggtatcgcag aagctctgtt agcaacggca atcggcctcg tggcagcgat tcctgcggtc
     1801 gttatctata acgtatttgc acgccagatt ggcggcttta aagcgatgct gggtgatgtt
     1861 gcagcgcagg tattgttgct gcaaagccgt gacctggatc tggaagccag cgccgctgcg
     1921 catccggttc gtgtcgcaca aaaattacgc gcaggataag gatctctaga gtcgacctgc
     1981 aggcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     2041 ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
     2101 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2161 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     2221 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     2281 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     2341 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     2401 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     2461 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     2521 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     2581 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     2641 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     2701 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     2761 tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     2821 tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
     2881 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     2941 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     3001 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     3061 agtcccctac gtgctgctga agttgcccgc aacagagagt ggaaccaacc ggtgatacca
     3121 cgatactatg actgagagtc aacgccatga gcggcctcat ttcttattct gagttacaac
     3181 agtccgcacc gctgccggta gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     3241 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgcccaacag
     3301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgccct gcaccattat
     3361 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     3421 aacgaagcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat catatcgtca
     3481 attattacct ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg
     3541 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     3601 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     3661 tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
     3721 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3781 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     3841 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3901 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3961 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     4021 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     4081 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     4141 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     4201 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4261 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4321 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4381 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     4441 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     4501 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     4561 attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
     4621 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     4681 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
     4741 gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
     4801 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
     4861 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     4921 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     4981 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     5041 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     5101 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     5161 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     5221 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     5281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     5341 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     5401 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     5461 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     5521 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     5581 atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
     5641 tcagccccat acgatataag ttgtaattct catgtttgac agcttatcat cgatgcataa
     5701 tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg
     5761 tcaattgtct gattcgttac caa
//
