LOCUS       sequence-31612-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB9B
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             649..666
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(719..737)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      733..780
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gttccgcgtg gtagtgggaa atcccttctc ttaaaggtca ttctcttggg
      121 tgatggtgga gttgggaaaa gttcgcttat gaaccgttac gtaaccaaca aatttgactc
      181 ccaggctttt cacaccatag gggtagagtt cttaaatcga gatctggagg tagatggacg
      241 ctttgtaacc ctccagatct gggacactgc agggcaggaa cgtttcaaga gccttaggac
      301 acccttctac aggggagcag actgctgcct cttgaccttc agcgtggatg atcggcagag
      361 cttcgagaat cttggtaact ggcagaaaga atttatttac tatgcggatg tgaaggaccc
      421 tgagcatttc ccctttgtag ttctgggtaa caaggtagac aaagaggata ggcaagtgac
      481 tactgaggag gcacaaacct ggtgcatgga gaatggggat tacccttatt tagaaactag
      541 tgccaaagat gatactaatg tgacagtggc ctttgaagaa gctgtcaggc aggtgctggc
      601 tgtagaggaa cagctggagt gatgacgaag cttgcggccg cactcgagca ccaccaccac
      661 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      721 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      781 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc g
//
