LOCUS       sequence-316152-        9348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018265
FEATURES             Location/Qualifiers
     source          1..9348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2052..2072
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2073..2096
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2122..2178
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2179..2196)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2179..2195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2210)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2404..2423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2523..2545
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2583..2601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3387..4586
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4817..5965)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6237..6608)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6641..6750
                     /note="incP origin of transfer"
     rep_origin      6903..7534
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7997..8585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8486..8505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8739..8756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8873..8894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8909..8914,8915..8932,8933..8939)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8947..8963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8952..8974
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8971..8987
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8971..8987
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaacc
      361 gcatcgtgcg gaatctggcg ttcggcggcc tgacattgct gctctgcctc ctgggcaccg
      421 cgggctgggc acaagacgat gaaccgagca agccggtcac cgacgtcttg ctcgccgccg
      481 accccgccga gagctggctc catacgaacg gcaactgggc ggggcagcgc tattctacgc
      541 tctcgcaact gaacgcctac aatgtcaagg gcctgaaggt cgcctggatg ttcagcaccg
      601 gtggcaagac cgatgcccag aacacccccc tctaccatga cggcctgatc tacttcgccc
      661 aggacaacca ggtcttcgcc atcaacgcgc gcagcgggaa gcaagtctgg ctgtacacgc
      721 atgagcttcc cgaagatttc ggaggctaca acgtcccctt catcacgggc aagcaccgcg
      781 gggtagcgct ctacggggag cacatctatt tcctgtccaa cgacaccaaa ctccatgcca
      841 tccactacaa gaccggccag cccaagttcg tcaagcagta cctcaagtac ccgaagaagt
      901 tcgaagaggc cgaggatgcg aatggctacg cgaccaccgt gggcagcctc gccatcccgg
      961 gtaagatcct cgtgccgctc aacgggacgg attggggcgg cctccccggc ttcgtctacg
     1021 cggttcaccc cgacaacggg gagatcctct ggcaggccaa catgatcccg ggcccgggcg
     1081 agccgggcgc cgagacgtgg cccggcgata gccgcgagta cggcggcgcc ggcccgtgga
     1141 tcacgggctc gtgggatccc gagctcaaga tgtactatac gggcaccgcc aacgcgtacc
     1201 cctggaaccc taagacgcgc ggggacggga agtacgataa cgtcggtgcc gcctcggtgg
     1261 tggccatcaa cacggataca ggcaaggtgg tctggcgcta cgtagtggtt ccgggcgacc
     1321 cgtgggacta cgacaccatg tcgaccccgg tcatcatcaa gctcgatggc gaaaaggtcc
     1381 tggtgcagcc gaacaagacc ggctacgtgc attacctgga tccgaagacc ggccagttcc
     1441 tgcgggcggt cgcattcgcg gacaagatca actgggcgaa gggctatgac gtggaggggc
     1501 ggccgatcga ccagatcgcg ctgccgaagg agggtgccga ccccgtggag gtgtggccca
     1561 gcttgctggg tgccgtcaac atgtacccca cctcgtacag cccgaagacg ggcctcatct
     1621 acctccccgc cgtcaacgct ggcatggagt acggttacga ggagatcaag acgatcagca
     1681 acgttcggca tttcggcgcc tatcaggagt tcatttgggg ctacgaggtc aatctcgccc
     1741 taaacccgca gaccggccag gaggtctggc gggaccagaa gagcaagcac ggctacgcgg
     1801 gcggcatgct ctcgacggcg ggtaacgtgg tgttctacac ctccgccagc ggccagttcc
     1861 acgccaccaa cgccgccacg ggagagatcc tctatacctt caacctggga accaccgcga
     1921 aggccggccc gatcaccttc atgctggacg gcaagcagta cgtcgtgcag gccgtgggcg
     1981 gagtcccggg cttcggatac gagggccaca acctgcagca tggtaacatg gtcgttgcgt
     2041 tcacccgagc cgaaaacctc tattttcagg gacatcatca ccatcaccat caccactgac
     2101 agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg
     2161 ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2221 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2281 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     2341 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     2401 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2461 tgacgcgccc tgacgggctt gtatgctccc ggcatccgct tacagacaag ctgtgaccgt
     2521 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     2581 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagca
     2641 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
     2701 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
     2761 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
     2821 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
     2881 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
     2941 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
     3001 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
     3061 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
     3121 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
     3181 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     3241 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
     3301 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
     3361 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga
     3421 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
     3481 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
     3541 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     3601 ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc atcatggcga
     3661 cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     3721 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     3781 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     3841 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     3901 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     3961 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     4021 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     4081 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     4141 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     4201 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     4261 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     4321 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     4381 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     4441 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     4501 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
     4561 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca
     4621 aggcgacttc cggcaagcta tacgcgccct agaattgtca attttaatcc tctgtttatc
     4681 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
     4741 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
     4801 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
     4861 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
     4921 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
     4981 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
     5041 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
     5101 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
     5161 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
     5221 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
     5281 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
     5341 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
     5401 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
     5461 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
     5521 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
     5581 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
     5641 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
     5701 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
     5761 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
     5821 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
     5881 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
     5941 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
     6001 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
     6061 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
     6121 tccgaatctt gccctgcacg aataccagct ccgcgaagtc gctcttcttg atggagcgca
     6181 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     6241 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     6301 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     6361 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     6421 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     6481 agcaggtagg cctacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     6541 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     6601 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     6661 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     6721 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     6781 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     6841 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgacccttt
     6901 ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca
     6961 aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga
     7021 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
     7081 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
     7141 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
     7201 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
     7261 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
     7321 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
     7381 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
     7441 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
     7501 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
     7561 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc tgaccacttc
     7621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9001 agctagcttc ccgcttggtc gggccgcttc gcgagggccc gttgacgaca acggtgcgat
     9061 gggtcccggc cccggtcaag acgatgccaa tacgttgcga cactacgcct tggcactttt
     9121 agaattgcct tatcgtcctg ataagaaatg tccgaccagc taaagacatc gcgtccaatc
     9181 aaagcctaga aaatataggc gaagggacgc taataagtct ttcataagac cgcgcaaatc
     9241 taaaaatatc cttagattca cgatgcggca cttcggatga cttccgagcg agcctggaac
     9301 ctcagaaaaa cgtctgagag ataccgcgga tctcacacag gaaatttc
//
