LOCUS       sequence-316168-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5..24)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     501..521
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             620..673
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             674..1375
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(1435..1455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1853..1864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2316..2333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2322..2546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2572..2592)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2572..2590)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2611..2627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2611..2627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2624..2646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2635..2651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2659..2665,2666..2683,2684..2689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2704..2725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2842..2859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3013..3601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3093..3112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3772..4563,4564..4632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4395..4414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4633..4737)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4763..5218)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4900..4921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5112..5131
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5345..5367
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5359..5376
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5360..5376
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5386..5405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5386..5404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5720..5918
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5873..5893
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5936..6116
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6163..6288
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6787..7020
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7205..7249
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7547..7664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7739..7987
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7739..7759
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7910..7929
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        8116..8615
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8151..8174)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8548..8567
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
       61 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
      121 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
      181 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
      241 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
      301 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
      361 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
      421 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
      481 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
      541 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
      601 cggcggcggg tccggaggag agggcagagg aagtctccta acatgcggtg acgtggagga
      661 gaatcctggc ccaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
      721 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
      781 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
      841 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
      901 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
      961 cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1021 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     1081 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     1141 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     1201 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     1261 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     1321 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta attgagcggc
     1381 cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1441 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1501 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     1561 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     1621 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     1681 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     1741 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     1801 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     1861 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     1921 acgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc aggtaccttt
     1981 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     2041 actggaaggg ctaattcact cccaaagaag tcaagatctg ctttttgcct gtactgggtc
     2101 tctctggtta gaccagagtc tctctggtta gaccagatct gagcctggga gctctctggc
     2161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2281 tggaaaatct ctagcagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     2341 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2401 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2461 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca atagcaggca
     2521 tgctggggat gcggtgggct ctatgggcgg ccgttaatta agcttttgtt ccctttagtg
     2581 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2641 tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
     2701 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2761 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2821 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2881 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2941 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3001 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3061 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3121 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3181 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3241 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3301 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3361 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3421 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3481 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3541 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3601 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3661 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3721 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3781 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3841 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3901 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3961 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4021 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4081 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4141 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4201 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4261 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4321 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4381 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4441 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4501 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4561 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4621 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4681 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4741 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4801 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4861 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4921 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4981 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5041 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5101 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5161 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     5221 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5281 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5341 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     5401 tagggcgaat tgactagtac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     5461 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5521 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     5581 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5641 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5701 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     5761 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5821 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     5881 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     5941 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6001 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6061 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     6121 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     6181 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     6241 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     6301 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     6361 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     6421 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     6481 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     6541 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     6601 aaaagtaaga ccaccgcaca gcaagcggcc ggccgcgctg atcttcagac ctggaggagg
     6661 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     6721 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     6781 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     6841 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     6901 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     6961 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7021 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7081 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7141 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7201 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7261 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     7321 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     7381 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     7441 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     7501 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     7561 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     7621 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     7681 acagcagaga tccagtttgg ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga
     7741 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     7801 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     7861 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     7921 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     7981 aaacaccgga actaacggtt cgagtgagtt taagagctat gctggaaaca gcatagcaag
     8041 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     8101 ggatccaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     8161 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     8221 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     8281 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     8341 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     8401 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     8461 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     8521 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     8581 cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta gatctcgagc
     8641 agctgaagct
//
