LOCUS       sequence-316180-        9158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG-sfGFP-iCre-ERT2 piggyBac transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-sfGFP-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1745..1747,1748..2458)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1745..2458
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1775..1803)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2398..2418
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3587..4522
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3635..4528
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    4565..5153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4618..4638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5036..5047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5192..5209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5198..5422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5776..5810
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5897..5916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5898..5916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5923..5940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5923..5939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5932..5954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6081..6536)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6218..6239)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6430..6449
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6563..6667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6668..6736,6737..7528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6886..6905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7699..8287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8188..8207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8441..8458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8575..8596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8611..8616,8617..8634,8635..8641)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8649..8665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8654..8676
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8673..8689
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8673..8689
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8708..8728
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8710..8728
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8792..8854
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crgggggggg gggggggggs gggggggggg
      541 gsggggsgrg gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggrg ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg ctagcgccgc
     1741 caccatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1801 tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa
     1861 cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac
     1921 acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa
     1981 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
     2041 tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct
     2101 tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca
     2161 caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa
     2221 tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc
     2281 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2341 ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt
     2401 ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg
     2461 aggatctggc ggccgctctg ccacaaactt cagcctgctg aagcaggccg gcgacgccgc
     2521 tgaaaatcct ggccctgctc ctggaagcac caacctgctg actgtgcacc aaaacctgcc
     2581 tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag
     2641 ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg
     2701 ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag
     2761 ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct
     2821 gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc
     2881 tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa
     2941 gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc
     3001 tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct
     3061 gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag
     3121 aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc
     3181 cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga
     3241 tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc
     3301 cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat
     3361 ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag
     3421 agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc
     3481 tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac
     3541 tggggccatg gtgaggctgc tcgaggatgg ggacctcgag ccatctgctg gagacatgag
     3601 agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc
     3661 cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact
     3721 ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac
     3781 caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt
     3841 tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct
     3901 gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa
     3961 cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat
     4021 gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg
     4081 cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa
     4141 gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat
     4201 ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct
     4261 cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag
     4321 catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca
     4381 ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca
     4441 cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga
     4501 ggcagagggt ttccctgcca cagcttgatt aattaatgat catacagtgc tctgagtacg
     4561 ctataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4621 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4681 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4741 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4801 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4861 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4921 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4981 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5041 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5101 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcaggatcc
     5161 actagagcgg ccgcctagag ctcgctatca gcctcgactg tgccttctag ttgccagcca
     5221 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5281 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5341 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5401 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga
     5461 gcttgcggaa cccttaagct tttttattat atattagtca cgatatctat aacaagaaaa
     5521 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     5581 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     5641 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     5701 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     5761 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga
     5821 tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc
     5881 acattgtgtt gggtcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     5941 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6001 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6061 cagcctgaat ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     6121 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     6181 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     6241 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     6301 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     6361 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     6421 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     6481 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     6541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6901 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7201 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8401 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8461 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8521 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8581 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8641 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     8701 caagcgcgca attaaccctc actaaaggga acctcccccg gtattcacga cagcaggctg
     8761 aataataaaa aaattagaaa ctattattta accctagaaa gataatcata ttgtgacgta
     8821 cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     8881 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     8941 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa
     9001 atttcttcta taaagtaaca aaacttttaa acattctctc ttttacaaaa ataaacttat
     9061 tttgtacttt aaaaacagtc atgttgtatt ataaaataag taattagctt aacctataca
     9121 taatagaaac aaattatact tattaaccgg tcatggtc
//
