LOCUS       sequence-316206-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contain promoter PthrC3_7, which is used for study 
            about Short, Auto-inducible Promoters for Well-Controlled Protein 
            Expression in Escherichia coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Anilionyte O, Liang H, Ma X, Yang L, Zhou K
  TITLE     Short, auto-inducible promoters for well-controlled protein 
            expression in Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Aug;102(16):7007-7015. doi: 
            10.1007/s00253-018-9141-z. Epub 2018 Jun 8.
  PUBMED    29948110
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9141-z"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-08"; volume: "102"; issue: "16"; pages: 
            "7007-7015"
COMMENT     Sequence Label: pMB1-spect-PthrC3_7-eGFP
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             559..581
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(589..591,592..1305)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(589..591,592..1302)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(631..652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(892..911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1239..1260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1666..2457
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2648..3236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3137..3156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
       61 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      121 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      181 tatgctggat ccttccctcg actcacactt gggagctttt cattctgact gcaacgggca
      241 atatgtctct gtgtggatta aaaaaagagt gtctgatagc agcttctgaa ctggttacct
      301 gccgtgagta aattaaaatt ttattgactt aggtcactaa atactttaac caatataggc
      361 atagcgcaca gacagataaa aattacagag tacacaacat ccatgaaacg cattagcacc
      421 accattacca ccaccatcac cattaccaca ggtaaaggtg cgggcttacg cgtacaggaa
      481 acacagaaaa aagcccgcac ctgacagtgc gggctttttt ttcgaccaaa ggtaacgagg
      541 taacaaccta gaaataattt tgtttaactt taagaaggag atatacatat gagcaagggc
      601 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      661 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      721 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      781 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      841 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      901 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      961 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1021 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1081 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1201 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agtgatgggc tgaagggttt
     1321 aagcgaagac gaacaataag gcctcccaaa tcggggggcc ttttttattg ataacaaaaa
     1381 ggcaacacta tgacatcgga aaacccgtta ctggcgctgc gagagaaaat cagcgcgctg
     1441 gatgaaaaat tattagcgtt actggcagag cggcgcgaat tcaaacagaa aggccatggg
     1501 tcgacctgca gaagcttaga tctattaccc tgttatccct actcgagttc atgtgcagct
     1561 ccataagcaa aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga
     1621 tcgtgctatg atcgactgat gtcatcagcg gtggagtgca atgtcatgag ggaagcggtg
     1681 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1741 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1801 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1861 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1921 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1981 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     2041 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     2101 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     2161 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     2221 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     2281 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     2341 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     2401 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataagat
     2461 gccgctcgcc agtcgattgg ctgagctcat aagttcctat tccgaagttc cgcgaacgcg
     2521 taaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtcac
     2581 acaacatagc cacggagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2641 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2701 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2761 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2821 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2881 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2941 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3001 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3061 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3241 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct c
//
