LOCUS       sequence-316226-        9372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018269
FEATURES             Location/Qualifiers
     source          1..9372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2076..2096
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2097..2120
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2146..2202
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2203..2220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2203..2219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2212..2234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2428..2447)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2547..2569
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2607..2625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3411..4610
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4841..5989)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6261..6632)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6665..6774
                     /note="incP origin of transfer"
     rep_origin      6927..7558
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8021..8609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8510..8529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8763..8780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8897..8918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8933..8938,8939..8956,8957..8963)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8971..8987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8976..8998
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8995..9011
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8995..9011
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaaga
      361 agcgctgggg ctacctgtcc ctccccgtcg tcgttgcgat cggcgccacc tttgcggcga
      421 gctcgaccgt cgcccagacc gtcgataccg cccggatcga gacgggcggc cagaacgact
      481 ggctgacgta ccacggctcc tacaagtcct atcactattc tccgctcagc cagatcaaca
      541 cctcgaacgt cggcaacctg tccgtggcct ggatccacat cccgggccgg tccacccggg
      601 gcctgcagag catgccgctg gcggccgacg gcgttctcta ctatacgggc tcgtactcgc
      661 gggttttcgc gctgaacggc gccacgggcg aggtcatctg gagctacttc ccggagctgg
      721 acgaggcgtt gatcgccagg cagacccatt cgccctacaa ccgtggcgcc gcccttggcg
      781 agggcaaggt ctacgtcggg accatggacg gccgccttat cgccctcgac atgaagactg
      841 ggaaggtggc ctgggacacc aagcttatcg actcccagaa gttgaccgtg ggcttcaccg
      901 gcgccccgct ctacgcgaag ggcacggtca tcatcggcgc gcagggcggt gaatggcccg
      961 gacggggacc gatcttcgcc gtcgacgccg cgacgggcaa gaaaaagtgg gagttcctga
     1021 cagtcgcggg caccgacgag gctatgaaga cgtggggcag tgactcctgg cgcaccggcg
     1081 ggggcggcgg ctggatgccg ggcacgtatg actcggagac gaacaccatc ctctggggca
     1141 cggcgaaccc ggccccgctg tatgattgga gcgggagcga ctataagacc cagggggccc
     1201 gtccgggcga caacctgtat accacgtcgg tgatcgggct ggacctcgac tcggggaaac
     1261 tgaagttcta ccaccaggag ctgccgcacg atgcctggga cttcgacagc gcggtggggg
     1321 agttcgtcat gctcgaccgc gacgggcaga agctcgtcgt gcatccgaac aagagcgggt
     1381 acatcttcgt ctacgaccgc aacctcggca tcaagaacgt gtggcggatc acccaaaaca
     1441 gcaacttcgt caaggacatc gacccgaaga ccggcgagct gattggccgc cgggactttt
     1501 ctgctggcaa ggtgaacgag ccgctgtgcc cgcacatctc aggtggagtc tcgttcaact
     1561 ctggctcgta taacccgaag accggcctct attacaagct cggccaggag tggtgcatga
     1621 cgctggaggt gatgaagacg acccctgtga ccgctccgca ggcccagctc aacatcggcg
     1681 ccgatttcaa gattgcgccg ccgcccggcg gcgaaattta cggccacctc gacgcccgag
     1741 atccggtcac cggcgtgaag aagtgggagg tccgataccc cgaacccccc ctgggctccg
     1801 tcctcagcac gggtgggaat ctcgtcttcg tgcccgattc gcgcggcacc ctgcatgcct
     1861 acgacgcgga gagcggggcc gaactctggt cgcactcgga tggtacggga caccagggtg
     1921 ggatcgtcag ctacgccgtc aacggtaagc aatacgtcgc ggtgaccgcg ggcttcggcg
     1981 gcatggcgag cgacgactac gccccgacgt tcggcggcgt ctacaagtcg atgccccgcg
     2041 atgacggcgc cctgatcgtc tacagcctga aggccgaaaa cctctatttt cagggacatc
     2101 atcaccatca ccatcaccac tgacagctat gaccatgatt acgccaagct tgcatgcctg
     2161 caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca
     2221 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2281 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2341 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2401 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2461 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtatgc tcccggcatc
     2521 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2581 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2641 catgataata atggtttctt agcacccttt ctcggtcctt caacgttcct gacaacgagc
     2701 ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga
     2761 ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca
     2821 acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca
     2881 acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc
     2941 tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg
     3001 ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca
     3061 ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc
     3121 tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag
     3181 taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct
     3241 acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt
     3301 gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta tcagtgataa
     3361 agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca
     3421 acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga
     3481 ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc
     3541 actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg
     3601 gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg gccggcgcca
     3661 ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat atcgggcgga
     3721 tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt gccgatatca
     3781 ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc gggttcggga
     3841 tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac gctccgttct
     3901 tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt
     3961 cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc gcttcgttcc
     4021 ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac
     4081 ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc tttcactggg
     4141 acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca ctcgcccagg
     4201 caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc atgctcggaa
     4261 tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga tggatggcgt
     4321 tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg caagcaatgt
     4381 tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg gcggcgctca
     4441 ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg gcttctataa
     4501 caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc tgcctgccgg
     4561 cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac
     4621 gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg ccctagaatt
     4681 gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat
     4741 actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc
     4801 tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
     4861 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
     4921 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
     4981 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
     5041 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
     5101 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
     5161 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
     5221 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
     5281 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
     5341 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
     5401 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
     5461 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
     5521 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
     5581 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
     5641 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
     5701 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
     5761 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
     5821 cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc
     5881 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
     5941 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
     6001 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
     6061 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
     6121 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agctccgcga
     6181 agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc
     6241 cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc
     6301 ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca
     6361 ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg
     6421 cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg
     6481 attttgtagc cctggccgac ggccagcagg taggcctaca ggctcatgcc ggccgccgcc
     6541 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
     6601 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     6661 ttagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     6721 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     6781 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     6841 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     6901 gcggaaaaga tccgtcgacc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
     6961 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     7021 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     7081 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     7141 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     7201 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     7261 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     7321 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     7381 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     7441 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     7501 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     7561 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     7621 aatggcagcc aagctgacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8761 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8821 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8881 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8941 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9001 acagctatga ccatgattac gccaagctag cttcccgctt ggtcgggccg cttcgcgagg
     9061 gcccgttgac gacaacggtg cgatgggtcc cggccccggt caagacgatg ccaatacgtt
     9121 gcgacactac gccttggcac ttttagaatt gccttatcgt cctgataaga aatgtccgac
     9181 cagctaaaga catcgcgtcc aatcaaagcc tagaaaatat aggcgaaggg acgctaataa
     9241 gtctttcata agaccgcgca aatctaaaaa tatccttaga ttcacgatgc ggcacttcgg
     9301 atgacttccg agcgagcctg gaacctcaga aaaacgtctg agagataccg cggatctcac
     9361 acaggaaatt tc
//
