LOCUS       sequence-316228-        9396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018273
FEATURES             Location/Qualifiers
     source          1..9396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2100..2120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2121..2144
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2170..2226
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2227..2244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2227..2243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2236..2258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2452..2471)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2571..2593
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2631..2649)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3435..4634
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4865..6013)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6285..6656)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6689..6798
                     /note="incP origin of transfer"
     rep_origin      6951..7582
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8045..8633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8534..8553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8787..8804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8921..8942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8957..8962,8963..8980,8981..8987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8995..9011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9000..9022
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9019..9035
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9019..9035
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcgcc
      361 gccacctcct gaccctcgcg ctgctcgccc tcgccctccc cgctgccgcg cagaaggatc
      421 gggactggcc caccttcggg ctcgaccacg ccaacacccg cctggcggac gtcccggcca
      481 tcacgccgaa gaccgtcaag aaactgcttc ccaagtggat ctaccagagc ggcgtcgccg
      541 cgaccttcca ggctacgccg attgtcacgg gcggccggat gtacgtctcc ctgcccggta
      601 gccatgtggt cgccctggat gcccgcagcg gcgcggaact gtggcggtac acgcaccgca
      661 agcgcacgga gaagctctgc tgcggcccgg cgaacagggg cgtggcggtc gacgacggcc
      721 gggtctttgt ggcgaccgtc gacgcgcgcc tggtggccct tgacgccgcg accgggaagc
      781 ccgtctggga catcgaactc gcccgccccg acgaggcgat caccgagagc ggcgaaggca
      841 tcaagggcga ggtgggcggg ggcagcggag tgggcgcggc ggctgccccg ctggtcgtcg
      901 acggcaaggt gatcgtgggc atcaatggtg tggggtacgg tctgcatatc gacgcccaag
      961 ggcgggccgg ccagctggcg gccgtcgtgg gcatcgcggg gaagtacggc ggcattggct
     1021 tcctggccgc cttcgacgcc aagacgggga agcgggtgtg gcagttcgac accgtccgca
     1081 agccgcagga cgggggatgg gagggcgact tcgtagaacg caccgccgac ggcctgccgc
     1141 tgaatcgcaa catagccgag gagcgcgcga acgcggggaa gtatgccgat gcctgggagt
     1201 acggcggggg gtcggtgtgg aacgccccgg cgtacgacgc gaagaccggc ctgctctatt
     1261 tcgggaccgg caacccgagc ccgcagatca acgactcgac gaggcccggg gacaacctct
     1321 acacggcgtc gctggtggct atcgacgcga agacgggacg gcatgcctgg cacttccagc
     1381 agatcccgca tgacaagtgg ggctacgacg tcgcctcgcc gcccctcctg ttcgagcacc
     1441 gggaggccga cggccgcgtc gtgcccgccg tgggccaggc gagcaagctc ggatggttct
     1501 atgtgcacga tcgcgcctcc ggcgccctcc tctacaaatc ggacgcgttc gtgccccagg
     1561 acaatttgtt cgcagccgcc tcccgcgagg gcacgcgcat cacgcccggg gtggccggcg
     1621 gcgtgaactg gtcgccggcg gccctggaca cgcagcgcgg cctggcgttc gtcgccgcga
     1681 tgcacatgcc gatgcgatac tggatcaagg agtcggccgc gaccgcggac aaacccgcgc
     1741 agcggtacga tagcatggag ccggccgacg aggcgcagtg gggcgtcctg gccgcgatcg
     1801 acctcaagaa gcggggccgc cttaagtgga ccgtcaagac cggccagccg ctgatcgggg
     1861 gggtcctcgc cctgaaaagc ggcgtggtct tcaccggcga aggcgacggc catctctcgg
     1921 ctttcgactc cctgtcgggc aagcgactct ggcgcttcca gacgggagcc ggagtgaacg
     1981 cccccccgat cgcctatgtg ctggacggga agccgtacgt ggccgttgcc gcggggggct
     2041 cggcgatctg gggctatcgc caaggggacg ccgtcgtggt cttcgggctc cctgacgccg
     2101 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ctatgaccat
     2161 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     2221 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2281 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2341 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     2401 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     2461 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     2521 acgggcttgt atgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     2581 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     2641 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcacc ctttctcggt
     2701 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     2761 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     2821 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     2881 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     2941 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     3001 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     3061 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     3121 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     3181 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     3241 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     3301 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     3361 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     3421 tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc
     3481 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     3541 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc
     3601 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct
     3661 tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca gcgcctttcc
     3721 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     3781 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     3841 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     3901 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     3961 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     4021 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     4081 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     4141 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     4201 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     4261 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     4321 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     4381 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     4441 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     4501 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     4561 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     4621 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     4681 gcaagctata cgcgccctag aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     4741 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
     4801 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
     4861 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     4921 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     4981 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     5041 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     5101 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     5161 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     5221 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     5281 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     5341 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     5401 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     5461 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     5521 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     5581 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     5641 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     5701 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     5761 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     5821 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
     5881 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     5941 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     6001 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     6061 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     6121 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     6181 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     6241 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     6301 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     6361 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     6421 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     6481 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     6541 tacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     6601 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     6661 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     6721 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     6781 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     6841 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     6901 atgaccccga agcagggtta tgcagcggaa aagatccgtc gaccctttcc gacgctcacc
     6961 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     7021 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
     7081 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     7141 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     7201 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     7261 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     7321 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     7381 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     7441 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     7501 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     7561 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     7621 ggccgcgaac ggcctcaccc caaaaatggc agccaagctg accacttctg cgctcggccc
     7681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8161 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8941 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9001 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctagcttccc
     9061 gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac ggtgcgatgg gtcccggccc
     9121 cggtcaagac gatgccaata cgttgcgaca ctacgccttg gcacttttag aattgcctta
     9181 tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc gtccaatcaa agcctagaaa
     9241 atataggcga agggacgcta ataagtcttt cataagaccg cgcaaatcta aaaatatcct
     9301 tagattcacg atgcggcact tcggatgact tccgagcgag cctggaacct cagaaaaacg
     9361 tctgagagat accgcggatc tcacacagga aatttc
//
