LOCUS       sequence-316232-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018279
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2106..2126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2127..2150
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2176..2232
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2233..2250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2233..2249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2242..2264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2458..2477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2577..2599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2637..2655)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3441..4640
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4871..6019)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6291..6662)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6695..6804
                     /note="incP origin of transfer"
     rep_origin      6957..7588
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8051..8639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8540..8559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8793..8810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8927..8948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8963..8968,8969..8986,8987..8993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9001..9017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9006..9028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9025..9041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9025..9041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgacct
      361 tccgcgcgac cctgctcgcc gcggccggcg ccgcggccct gctcgcgggc gtcgccagcc
      421 cggcactcgc cgtgacggac gaggacatcg ccaacgatgc caaaacgacc gaggacgtcg
      481 tcacctatgg tctgggcaac cagggccagc gcttcagccc gcttaagacg ctcactcgcg
      541 acaacatcaa gtcgctcgtc cccgcctaca gcttctcgtt cgggggagag aagcagcgtg
      601 gccaagagtc acaggccctc gtcaaggacg gcgtgatcta cgtgaccggc tcgtactccc
      661 gcgcgtgggc gatcaactcc cgcacgggcg aaaagatctg ggagtacaac gcccgtctcc
      721 cggagggaat cttgccgtgc tgcgatgtgg tcaaccgcgg cggggccctg tacgacaacc
      781 tgttcatctt cggcaccctg gacgcgaagc tcgtggcgct ggatgccaag tccggcaaag
      841 tcgtgtggaa gaaagacgtg gacgacttca agtccggttt tagcttcacc agcgcgccga
      901 tgatcgtgaa gggtaagatc atcaccgggg tctcgggcgg tgagttcggg atcgtcggcc
      961 gggtggaggc ccgcgacgcc aagacggggg aaatcatctg gaagcgcccg gtgatcgagg
     1021 gccacgtggg cgagctgaac ggcaagccaa gcacgatgac cggcaagacg aacgagacct
     1081 ggcccggcga tatgtggaag tacgggggcg gagccacgtg gaacggcggc acctatgacg
     1141 cagagaccaa cctgatctac tacgggacgg gcaatccggg cccgtggaac tcgtggctgc
     1201 ggccgggcga caacaagtgg tcggcctcgc ggatcgccct ggacccggac acgggcgaga
     1261 tcaagtgggg gttccagacg accccgcatg atggctggga ctatgatggt gtcaacgaat
     1321 tcgtctcgtt cgacctcgtg aaggacggca agaccatcaa ggcgggcgcg acggcggacc
     1381 gtaacggctt cttctacgtc ctggaccgca cgaacggcaa gttcatctcc gccagtccat
     1441 tcgtgagcaa gatcacctgg gcgaagggca tcgacgagaa gggccgtcct atcgaggacc
     1501 cggccttccg ccccgccgat ccgggaaagg aaaagcaagg taaatccgtg ttcgccgtcc
     1561 cgagcttcct gggcggcaag aactggatgc ccatcggcta cagcccggag acgaagctct
     1621 tctacgtccc gagcaacgaa tggggcatgg atatccacaa cgagccggtg gcgtataaaa
     1681 aaggcgcagc ctaccttggc gctggcttca cgatcaagcc tatcttcgag gaccatatcg
     1741 ggtccctcaa ggccatcgac ccggcgaccg gaaagattgc gtgggagtat aagaacaaag
     1801 ccccgctctg gggcggcgtg ctctcgacgg cgggcaacct cgtgttcacg ggcaccccgg
     1861 aaggtttcct gaaagccttc gacgccaaga cgggggagga ggtctggaag ttcaacgtcg
     1921 gcacgggggt cgtggccacc cccatcacct gggagcagga cggggagcag tgggtgggcg
     1981 tcgccgccgg ctggggcggc gcggtgccgc tgtggggcgg cgaggtcgcc aagtcgacgg
     2041 ccggtatcac ccagggcggc tcgttctggg cgttcaagct cccgaaggaa gtggccgccc
     2101 gggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagctat
     2161 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     2221 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2281 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2461 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2521 gccctgacgg gcttgtatgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2581 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2641 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
     2701 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     2761 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     2821 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     2881 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     2941 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     3001 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     3061 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     3121 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     3181 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     3241 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     3301 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     3361 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     3421 ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg
     3481 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     3541 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     3601 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     3661 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     3721 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     3781 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     3841 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     3901 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     3961 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     4021 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     4081 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     4141 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     4201 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     4261 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     4321 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     4381 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     4441 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     4501 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     4561 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     4621 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     4681 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     4741 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     4801 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     4861 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     4921 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     4981 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     5041 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     5101 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     5161 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     5221 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     5281 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     5341 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     5401 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     5461 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     5521 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     5581 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     5641 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     5701 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     5761 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     5821 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     5881 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     5941 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     6001 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     6061 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     6121 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     6181 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     6241 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     6301 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     6361 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     6421 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     6481 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     6541 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     6601 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     6661 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     6721 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     6781 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     6841 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     6901 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg
     6961 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     7021 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     7081 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     7141 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     7201 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     7261 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     7321 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     7381 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     7441 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     7501 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     7561 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     7621 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc aagctgacca cttctgcgct
     7681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8161 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8641 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8701 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8761 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8821 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8881 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8941 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9001 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag
     9061 cttcccgctt ggtcgggccg cttcgcgagg gcccgttgac gacaacggtg cgatgggtcc
     9121 cggccccggt caagacgatg ccaatacgtt gcgacactac gccttggcac ttttagaatt
     9181 gccttatcgt cctgataaga aatgtccgac cagctaaaga catcgcgtcc aatcaaagcc
     9241 tagaaaatat aggcgaaggg acgctaataa gtctttcata agaccgcgca aatctaaaaa
     9301 tatccttaga ttcacgatgc ggcacttcgg atgacttccg agcgagcctg gaacctcaga
     9361 aaaacgtctg agagataccg cggatctcac acaggaaatt tc
//
