LOCUS       sequence-31629-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kim JS, Bonifant C, Bunz F, Lane WS, Waldman T
  TITLE     Epitope tagging of endogenous genes in diverse human cell lines.
  JOURNAL   Nucleic Acids Res. 2008 Nov . 36(19):e127.
  PUBMED    18784188
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2008 Nov . 36(19):e127."
COMMENT     Sequence Label: pAAV-SEPT-Acceptor
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..60
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    157..291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(194..213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     248..267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(424..443)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 atgcacagtg gtaccttaaa atttggataa cttcgtataa tgtatgctat acgaagttat
       61 tcccaactca tcccggcctc gccggggccc cctagaaaaa acctcccaca cctccccctg
      121 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cccccgaacc
      301 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
      361 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
      421 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
      481 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
      541 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
      601 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
      661 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
      721 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
      781 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
      841 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
      901 ccacgatagc cgcgctgcct cgtcctgcag ttcattcngg caccgga
//
