LOCUS       sequence-31637-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Mouse Twisted Gastrulation
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2 mouse twisted gastrulation
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(812..831)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     865..884
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 atcgattcga attcatgcag tgtcccccca tcctacttgt gtggaccctg tggattatgg
       61 ctgtggattg ctctcgccca aaggttttct tgcccattca gcctgaacag gagcccctcc
      121 aatccaagac tgactacaag gacgacgatg acaagctcga gtgtaacaaa gcactctgtg
      181 ccagcgatgt gagcaaatgc ctcattcagg agctctgcca gtgccggcct ggagaaggga
      241 actgcccctg ctgtaaggag tgcatgctgt gcctcggggc cctgtgggac gagtgctgcg
      301 actgtgtcgg tatgtgcaac cctcggaatt acagcgacac cccgcccaca tccaagagca
      361 ccgtggagga gctgcacgag cccattccgt ccctgttcag ggcgctgacg gagggcgaca
      421 cccagctgaa ctggaacatc gtctccttcc ctgtggcaga ggagctgtca caccatgaaa
      481 acctagtctc cttcctagaa actgtgaacc agctgcacca ccaaaacgtg tctgttccca
      541 gcaacaatgt ccacgccccc ttccccagcg acaaagagcg catgtgcaca gtggtttact
      601 ttgatgactg catgtccatc caccagtgta agatatcctg cgaatccatg ggtgcatcca
      661 agtatcgctg gtttcacaac gcctgctgcg agtgcatcgg tccagagtgc attgactatg
      721 ggagtaaaac tgtcaagtgt atgaactgca tgttttaatc tagaactata gtgagtcgta
      781 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      841 gcagtgaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccat
//
