LOCUS       sequence-31639-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Mouse Twisted Gastrulation
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEM mouse twisted gastrulation
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(730..748)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(731..748)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(766..782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(766..782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(779..801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    790..806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgctccggcc gccatggcgg ccgcgggaat tcgattggct cgagtgtaac aaagcactct
       61 gtgccagcga tgtgagcaaa tgcctcattc aggagctctg ccagtgccgg cctggagaag
      121 ggaactgccc ctgctgtaag gagtgcatgc tgtgcctcgg ggccctgtgg gacgagtgtt
      181 gcgactgtgt cggtatgtgc aaccctcgga attacagcga caccccgccc acatccaaga
      241 gcaccgtgga ggagctgcac gagcccattc cgtccctgtt cagggcgctg acggagggcg
      301 acacccagct gaactggaac atcgtctcct tccctgtggc agaggagctg tcacaccatg
      361 aaaacctagt ctccttccta gaaactgtga accagctgca ccaccaaaac gtgtctgttc
      421 ccagcaacaa tgtccacgcc cccttcccca gcgacaaaga gcgcatgtgc acagtggttt
      481 actttgatga ctgcatgtcc atccaccagt gtaagatatc ctgcgaatcc atgggtgcat
      541 ccaagtatcg ctggtttcac aacgcctgct gcgagtgcat cggtccagag tgcattgact
      601 atgggagtaa aactgtcaag tgtatgaact gcatgtttta atctagagca atcactagtg
      661 aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag
      721 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccgga
//
