LOCUS       sequence-31641-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag
            J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A, 
            Anderson DJ
  TITLE     Genetic dissection of an amygdala microcircuit that gates 
            conditioned fear.
  JOURNAL   Nature. 2010 Nov 11. 468(7321):270-6.
  PUBMED    21068836
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Nov 11. 468(7321):270-6."
COMMENT     Sequence Label: pAAV-EF1a-FLEX-GTB
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    29..62
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    125..158
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggatcctct agagccgcca ccatggccat aacttcgtat aaagtatcct atacgaagtt
       61 atatcaaaat aggaagacca atgcttcacc atcgacccga attgccaagc atcaccatcg
      121 acccataact tcgtataatg tatgctatac gaagttatac ttcaactagt tcacagtctg
      181 gtctcgccgc cgctcttgtg gctctcccag ctgctgatga tcttgccgct ctggggggtc
      241 acgctcacct ctctgccggt gcctctcagg ttgtgctggg tgggctcgct tctgttcact
      301 cttctgcagc aggtcatcag gaagatgatc agcatcaggg cggtcagggc gccggcgctc
      361 agcagcacgt acttgcccca gttgggcagg cccaggtcca cgccgctcac ctggttgtgc
      421 acgtcgggca ggtgcacctc cacgaagtcc tcggcctcgt cgccgtcctt gaacacggtg
      481 ctggggtcgg ccagggggtg caccaggggg atcacgctgc tctccagcag ctccatgtgc
      541 tgctgcagca ggctgctctg catctcgggg atcagcacgt tgccgtcggg gcccaggatg
      601 atgccgttga agaacacgcc gttcacgtgg gggtggcatc tgccgcccac tctcaggcag
      661 cccttgctgg gcaggatctc gttccaggtt ctcacgctct tgtagtgggc gtcggcctcc
      721 atca
//
