LOCUS       sequence-31643-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM
  TITLE     Engineered allosteric activation of kinases in living cells.
  JOURNAL   Nat Biotechnol. 2010 Jul;28(7):743-7. doi: 10.1038/nbt.1639. Epub 
            2010 Jun 27.
  PUBMED    20581846
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1639"; 
            journalName: "Nat Biotechnol"; date: "2010-07"; volume: "28"; issue:
            "7"; pages: "743-7"
COMMENT     Sequence Label: pCMV5-Flag-RapR-p38
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..94
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctcgttta gtgaccgtca gaattcagat ctggtaccac gcgtatcgat aagcttcggc
       61 agccaacatg gactataagg acgatgatga caaaatgtcg caggagaggc ccacgttcta
      121 ccggcaggag ctgaacaaga ccatctggga ggtgcccgaa cgataccaga acctgtcccc
      181 ggtgggctcg ggcgcctatg gctcggtgtg tgctgctttt gatacatacc tgagcccagg
      241 cacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg attcctcccg
      301 ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga
      361 agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta
      421 tgcctatggt gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga
      481 tgtggagctt ctaaaactgg aaggcaatcc agctttgacg gggcatcgtg tggcagttaa
      541 gaagctgtcg agaccgtttc agtccatcat tcacgccaaa aggacctacc gagagttgcg
      601 tctgctgaag cacatgaaac acgaaaatgt gattggtctg ttggatgtgt tcacacccgc
      661 aaggtcactg gaggaattca atgacgtgta cctggtgacc catctcatgg gggcggacct
      721 gaacaacatc gtgaagtgcc agaagctgac cgacgaccac gttcagtttc tcatctacca
      781 gatcctccga gggctgaagt atatacattc ggctgacata attcacaggg acctaaagcc
      841 cagcaaccta gctgtgaacg aa
//
