LOCUS       sequence-316477-        8601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Hung YP, Albeck JG, Tantama M, Yellen G
  TITLE     Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
            Fluorescent Biosensor.
  JOURNAL   Cell Metab. 2011 Oct 5;14(4):545-54.
  PUBMED    21982714
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT     Sequence Label: GW1-Peredox-mCitrine
FEATURES             Location/Qualifiers
     source          1..8601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          720..1546
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             join(1770..2381,2382..2402,2403..3137,3138..3140,3141..3770,
                     3771..3815)
                     /codon_start=1
                     /product="genetically encoded fluorescent biosensor of the 
                     cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
                     /label=Peredox
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
                     KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
                     ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
                     IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
                     KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
                     VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
                     YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR
                     PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
                     KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     CDS             join(3816..3818,3819..3821,3822..4535)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     4469..4490
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4657..4791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4694..4713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4748..4767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4814..4830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4814..4830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4827..4849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4838..4854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4862..4868,4869..4886,4887..4892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5044..5061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5215..5803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5295..5314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5974..6765,6766..6834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6597..6616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6891..7404)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7086..7107)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7298..7317
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7576..7594
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7632..7654)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7754..7773
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8005..8027
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8019..8036
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8020..8036
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgca tgctatactg
      781 tttttggctt ggggtctata cacccccgct tcctcatgtt ataggtgatg gtatagctta
      841 gcctataggt gtgggttatt gaccattatt gaccactccc ctattggtga cgatactttc
      901 cattactaat ccataacatg gctctttgcc acaactctct ttattggcta tatgccaata
      961 cactgtcctt cagagactga cacggactct gtatttttac aggatggggt ctcatttatt
     1021 atttacaaat tcacatatac aacaccaccg tccccagtgc ccgcagtttt tattaaacat
     1081 aacgtgggat ctccacgcga atctcgggta cgtgttccgg acatgggctc ttctccggta
     1141 gcggcggagc ttctacatcc gagccctgct cccatgcctc cagcgactca tggtcgctcg
     1201 gcagctcctt gctcctaaca gtggaggcca gacttaggca cagcacgatg cccaccacca
     1261 ccagtgtgcc gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag ctcggggagc
     1321 gggcttgcac cgctgacgca tttggaagac ttaaggcagc ggcagaagaa gatgcaggca
     1381 gctgagttgt tgtgttctga taagagtcag aggtaactcc cgttgcggtg ctgttaacgg
     1441 tggagggcag tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata
     1501 gctgacagac taacagactg ttcctttcca tgggtctttt ctgcagtcac cgtccttgac
     1561 acgaagcttc ccggccacag caatcgatcc atgacacaac cagtgacaag cagtccccca
     1621 gtgaaaccgc atccgcatcc gagtccgata ccgataaaga ttctgaatcg gagtgagtgc
     1681 cgcgtccgag agcgttccct gtccacgtcc accatcggcg gagcaggtgt gcctgaggcc
     1741 tctagattgg gcccaccatg gttgctagca aagttcctga agcagccatt tccagactga
     1801 ttacttacct gagaattctg gaagagctgg aagcccaagg tgtacaccgc accgcctccg
     1861 agcaactggg cgagctggcc caggtcaccg ccttccaggt tgataaggac ctgtcctact
     1921 tcggctccta cggcactgac ggcgttggct acactgtacc ggtcctcaag agagaactgc
     1981 gccatatcct cggtctgaac cgcaaatggg gcctgtgcat cgtggggatg ggccgcctgg
     2041 gatccgctct tgctgattgg cctggttttg gcgagagctt cgagctgcgc ggtttcttcg
     2101 atgttgaccc aggtatggtt ggtcggccgg ttcgcggtgg tgttatcgaa cacgttgatc
     2161 tgctgcccca gcgcgtacct ggtcgcatcg aaatcgctct gctgacggtt ccacgcgagg
     2221 cagcacagaa ggctgccgac ctgctggttg ccgctggcat caagggcatc ctgaacttcg
     2281 ctccagttgt actggaggtt cccaaggaag tggctgttga gaacgttgac atcctggccg
     2341 gtctgacgcg tctgagcttt gccattctga accccacgtg gagcgcagca ggtgggcatg
     2401 gtttcactgc tcacaacgtg tatatcatgg ctgacaaaca gaagaacggt atcaaggcca
     2461 acttcaagat ccgccacaac atcgaagacg gcggcgttca gctggctgat cactaccagc
     2521 agaacacccc aatcggcgac ggccctgttc tgctgcctga caaccactac ttaagcatcc
     2581 agtctaaact gtccaaagac ccaaacgaga agcgcgacca catggttctg ctggaatttg
     2641 ttaccgccgc tggcatcacc cacggcatgg acgaactgta caagggcggc accgggggat
     2701 ctatggtttc caaaggcgag gaactgttca ctggtgttgt tcctatcctg gttgaactgg
     2761 acggcgacgt taacggccac aaattcagcg ttagcgggga aggcgaagga gacgctacct
     2821 acggcaagct gactctgaag ttcatctgca ccactggcaa gctgcctgtt ccttggccta
     2881 ccctggttac taccttctcc tatggtgtta tggttttcgc cagatatcct gaccacatga
     2941 agcagcacga cttcttcaag agcgccatgc ctgagggcta cgttcaggaa cgcaccatct
     3001 ttttcaagga cgacggcaac tataagacac gcgctgaggt taagttcgag ggcgacactc
     3061 tggttaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctgggcc
     3121 ataagcttga atataacacc aaggtacctg aagccgccat cagccgcctc atcacttacc
     3181 tccgcatcct ggaggagctc gaagcccaag gcgttcaccg cactgcaagc gagcagctgg
     3241 gcgaactggc acaggttaca gcattccagg ttgacgaaga tctgtcctat ttcggttcct
     3301 acggcaccga cggtgttggc tacacagtcc ctgtcctgaa gcgcgagctg cgccacatcc
     3361 tgggcctgaa ccgcaagtgg ggcttatgca tcgttggcat gggccgcctc ggtagtgcac
     3421 tggcagactg gccgggcttt ggtgaatctt tcgaactgcg cggcttcttc gacgttgatc
     3481 caggcatggt tgggcgccct gttcgcggtg gcgttatcga gcatgttgac ctcctgccac
     3541 agcgcgtccc gggtcgcatc gagatcgccc tgctgaccgt tcctcgcgaa gctgcacaga
     3601 aggcagccga cttactagtc gccgctggta tcaagggaat tctgaacttc gcccctgttg
     3661 ttctggaggt tcctaaggaa gttgctgttg aaaacgttga tttcctggcc ggcctgaccc
     3721 gcctgagctt cgcaatcctg aatcctaagt ggcgcgaaga aatgatgggt tccggaactg
     3781 ggggcaacgc ctctgacggt ggtgggtctg gtggtatggt gagcaagggc gaggagctgt
     3841 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3901 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3961 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
     4021 tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4081 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4141 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4201 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4261 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     4321 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4381 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga
     4441 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgctgctg
     4501 ggatcactct gggtatggat gaactgtaca agtaactcga cgaattcatt gatcataatc
     4561 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4621 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtaccgag
     4801 ctcgcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4861 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4921 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6841 cctttttcaa taagcggccg cggccatgcc ggccactagt ctcgagggcc accccggttg
     6901 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaagcg
     6961 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7021 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7081 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7141 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7201 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7261 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7321 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7381 ttaatgcgcc gctacagggc gcgtggggat ccctcgagtt attattgaag catttatcag
     7441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7501 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7561 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     7621 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7681 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7741 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gaagacgtcg
     7801 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
     7861 aaaaaaatta gtcagggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7921 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7981 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgccc
     8041 gtgtcaagga cggtgactgc agtgaataat aaaatgtgtg tttgtccgaa atacgcgttt
     8101 tgagatttct gtcgccgact aaattcatgt cgcgcgatag tggtgtttat cgccgataga
     8161 gatggcgata ttggaaaaat cgatatttga aaatatggca tattgaaaat gtcgccgatg
     8221 tgagtttctg tgtaactgat atcgccattt ttccaaaagt gatttttggg catacgcgat
     8281 atctggcgat agcgcttata tcgtttacgg gggatggcga tagacgactt tggtgacttg
     8341 ggcgattctg tgtgtcgcaa atatcgcagt ttcgatatag gtgacagacg atatgaggct
     8401 atatcgccga tagaggcgac atcaagctgg cacatggcca atgcatatcg atctatacat
     8461 tgaatcaata ttggccatta gccatattat tcattggtta tatagcataa atcaatattg
     8521 gctattggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
     8581 gtccaacatt accgccatgt t
//
