LOCUS       sequence-316480-        9408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018285
FEATURES             Location/Qualifiers
     source          1..9408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2112..2132
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2133..2156
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2182..2238
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2239..2256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2239..2255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2248..2270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2464..2483)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2583..2605
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2643..2661)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3447..4646
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4877..6025)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6297..6668)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6701..6810
                     /note="incP origin of transfer"
     rep_origin      6963..7594
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8057..8645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8546..8565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8799..8816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8933..8954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8969..8974,8975..8992,8993..8999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9007..9023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9012..9034
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9031..9047
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9031..9047
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagc
      361 gctcgaccct cgccccgttc atcctggcgg ccttcgcgca ctcgggcccc gtgcatgccg
      421 gcgtgacgac ggccgatatc gagaacgacg cccggaccac cggcgacgtc ctgtcgtggg
      481 gcatgggcac ggagggccag cgctattccc cgctcaccaa gatcaatacg aagaacgtca
      541 agcgtctggt gccggtctgg tcgtattcgt acggtggcga gaagcagcgc ggccaggagg
      601 cgcagccgct cgtcgtcaac ggcaaaatgt ttatcacggc ctcgtacagt cgcatcttcg
      661 cgctggacac caagacgggg cgcaaactct ggtcatacga gcaccgcctg ccggaaggca
      721 ttatgccgtg ttgcgacgtc atcaaccgtg gtgccgccgt gtatgataac ctggtcatct
      781 atgccaccct ggacgcccag ctcgtggcgc tggaccagaa cacggggaag gtcgtgtgga
      841 aggagaagat cgacgactac caggccggat actcgtcctc cgcggccccg atcattgcca
      901 aggggctggt gctcaccggc gtcagcggcg gcgagttcgg catcatcggg cgcgtcgagg
      961 cccgggatgc caagaccgga aagatggtgt gggtgcggcc gacgatcgag ggccatatgg
     1021 gctacaagtt cgatgagtct gggaacaaag tcgacaacgg catctccggc accacgaacg
     1081 cgagctggca aggcgacctc tggaagaccg gcggggcggc gacgtggctc ggcgggacgt
     1141 tcgacgccaa gaccggcctg gcgtacttcg ggaccggcaa ccccgccccg tggaattcgc
     1201 acctccgccc cggcgacaac ctctatagct gcagcaccgt cgccatcgac gtcgccacgg
     1261 gccagatcaa gtggcacttc cagggcacgc ccaacgacgg ctgggactac gacggcacca
     1321 acgagttcgt gacgttcgac gccggtggga agcgcctggg cgccaaggcc gaccggaatg
     1381 gcttcttcta cgtcctcgac gccaatacgg gtaagttcga gcgcggcttt ccgttcgtca
     1441 agaagcttga ttgggcgaaa ggcctcgacg agaacggtcg cccgatctac gaccccgcca
     1501 agcggcccgg cgatccgacc gcatccgaag acggcaagaa gggctccgtg gtccatgtct
     1561 cgccgtcgtt cctgggcggc aagaaccaga tgccgatggc ctatagcccg gacaccgggc
     1621 tgttctatgt ccccgccaac gagtggtcga tggagatctg gaacgagccg atcacgtata
     1681 agaagggcgc ggcctacctc ggcgcggggt tcaccatcaa gaccgctgac ccgaaggaaa
     1741 tcggcggcga ctatatcggc gcgctccgag cggtcgaccc caagtccggt aaggtggtgt
     1801 gggaaatcac gaacaacgcc ccgctctggg gcggcgccat gacgaccaaa ggcgggctgg
     1861 tgttctgggg caccccggag ggcttcttca aggccgcgga tgcgaagacg ggcaaggagc
     1921 tgtggtcgtt ccaggtcggc acgggcatcg tggcgccccc catcacctgg gagcaggacg
     1981 gtgagcagta cgtcgccgtc accgccggct ggggtggcgc ggtccccctg tgggggggcg
     2041 atgttgcgaa gaaggtctcc tacctcaacc agggcgggtc catgtgggtg ttcaagctca
     2101 tgaaggacgc cgaaaacctc tattttcagg gacatcatca ccatcaccat caccactgac
     2161 agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg
     2221 ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2281 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2341 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     2401 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     2461 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2521 tgacgcgccc tgacgggctt gtatgctccc ggcatccgct tacagacaag ctgtgaccgt
     2581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     2641 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagca
     2701 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
     2761 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
     2821 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
     2881 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
     2941 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
     3001 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
     3061 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
     3121 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
     3181 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
     3241 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     3301 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
     3361 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
     3421 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga
     3481 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
     3541 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
     3601 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     3661 ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc atcatggcga
     3721 cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     3781 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     3841 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     3901 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     3961 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     4021 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     4081 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     4141 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     4201 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     4261 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     4321 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     4381 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     4441 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     4501 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     4561 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
     4621 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca
     4681 aggcgacttc cggcaagcta tacgcgccct agaattgtca attttaatcc tctgtttatc
     4741 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
     4801 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
     4861 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
     4921 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
     4981 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
     5041 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
     5101 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
     5161 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
     5221 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
     5281 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
     5341 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
     5401 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
     5461 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
     5521 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
     5581 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
     5641 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
     5701 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
     5761 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
     5821 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
     5881 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
     5941 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
     6001 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
     6061 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
     6121 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
     6181 tccgaatctt gccctgcacg aataccagct ccgcgaagtc gctcttcttg atggagcgca
     6241 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     6301 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     6361 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     6421 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     6481 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     6541 agcaggtagg cctacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     6601 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     6661 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     6721 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     6781 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     6841 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     6901 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgacccttt
     6961 ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca
     7021 aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga
     7081 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
     7141 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
     7201 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
     7261 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
     7321 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
     7381 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
     7441 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
     7501 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
     7561 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
     7621 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc tgaccacttc
     7681 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7741 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7801 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7861 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8761 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8821 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8881 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8941 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9001 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9061 agctagcttc ccgcttggtc gggccgcttc gcgagggccc gttgacgaca acggtgcgat
     9121 gggtcccggc cccggtcaag acgatgccaa tacgttgcga cactacgcct tggcactttt
     9181 agaattgcct tatcgtcctg ataagaaatg tccgaccagc taaagacatc gcgtccaatc
     9241 aaagcctaga aaatataggc gaagggacgc taataagtct ttcataagac cgcgcaaatc
     9301 taaaaatatc cttagattca cgatgcggca cttcggatga cttccgagcg agcctggaac
     9361 ctcagaaaaa cgtctgagag ataccgcgga tctcacacag gaaatttc
//
