LOCUS       sequence-316500-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018291
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2121..2141
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2142..2165
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2191..2247
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2248..2265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2248..2264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2473..2492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2592..2614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2652..2670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3456..4655
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4886..6034)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6306..6677)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6710..6819
                     /note="incP origin of transfer"
     rep_origin      6972..7603
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8066..8654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8555..8574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8808..8825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8942..8963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8978..8983,8984..9001,9002..9008)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9016..9032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9021..9043
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9040..9056
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9040..9056
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgatcg
      361 tgcagaagaa gatcatgggc atcctggtgg gcctgggact ggcgtccctg gcgagcctgg
      421 cccaggccgc ggacgtcggc aacgtgacca acgccatgct ggactcgacg gccaagaacc
      481 cggcgagcgt cctctccttc ggcatgggga cccagggtca gcgctactcg cccctcaccc
      541 agatcaatga caagacggtc gcgaagctgg tgccggcgtg gtcgttctcg ttcggcggcg
      601 agaagcagcg tggccaggaa agccagccgc tcatccacaa cggcaagatg ttcgtgacgg
      661 cgagctacag ccgcattttc gccgtcgacc tcaagacggg cgcgaagctc tggaagtacg
      721 agcatcgcct gccggatggc atcatgccgt gctgcgacgt cgtgaaccgg ggcgccgcgc
      781 tgtatggcaa cctggtcatc ttcggtaccc tggacgccta tctcgtcgcc ctggaccaga
      841 acacgggcaa gatcgtgtgg aaggagaagg tcgacgatta cgccgccggt tacagcatga
      901 ccgccgctcc cctcatcgcg gagggcctcc tgctcaccgg agtgagcggc ggcgagtttg
      961 gcatcgtggg ccgggtggag gcgcgcaacc cgatgacggg cgacctcgtg tggacgcggc
     1021 ccacggtgga aggccatatg ggccatcgct atgacaaaga cggcaaggcg atcgacaacg
     1081 gcatctcggg cacggtgaac aagacctggc cgggcgacct gtggaagacc gggggtgcgt
     1141 ccacgtggat gggtgggacc tacgatcccc agaccaagtt ggcgtatttc ggcaccggca
     1201 acccggcgcc gtggaactcc cacctgaggc ccggcgacaa cctgtactcc tcgtccaccg
     1261 tcgcccttga cgtgaagacg ggccagatca agtgggccta ccagaacacc ccgaacgacg
     1321 cctgggactt cgacggtgcg aacgagttcg tcacctttga catggacggg aagcgctacg
     1381 gcggcaaggc cgaccgcaac ggcttcttct acgtcatcga cgcgagcacg ggcaagctcc
     1441 agaacgcgtt cccgttcgtc aagaagatca cgtgggccag ctcgatcgac cttaagaccg
     1501 ggcgcccgaa ctacatcgcg gccggccgcc cgggcgaccc caccaaggga gaggagggca
     1561 agaagggcac aacggtgttc gctgcccccg ccttcctggg cgcgaagaac cagatgccga
     1621 tggcgtactc gccgcagacc aagctcttct acgtgccggc caatgagtgg ggcatggaca
     1681 tctggaacga gcccatttcc tacaagaagg gcggcgcctt ccttggcgcc ggcttcacga
     1741 tcaagcccct gttcgacgac tacatcgggg ccatgcgcgc cgtggaccca aagaccggca
     1801 agatcgtgtg ggagatcaaa aacaacgccc ccttgtgggg gggcgtcatg agcacagcgg
     1861 gcaacctcgt cttctacggc acgccggaag gtttcctgaa ggcggtggac gcgaagactg
     1921 gtaaggagct gtggaagttc cagaccggca gcggggtcgt cgccccgccg gtcacctggc
     1981 aggacggcga tacgcagtac gtggcggtgg tgagcggctg gggaggtgcg gtcccgctct
     2041 ggggcggcga ggtggcgaag aaggtgaatt tcctcgaaca aggcggctcg gtctgggtgt
     2101 tcaagctggc gtccaaggcc gaaaacctct attttcaggg acatcatcac catcaccatc
     2161 accactgaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga
     2221 ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
     2281 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2341 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2401 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     2461 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2521 aacacccgct gacgcgccct gacgggcttg tatgctcccg gcatccgctt acagacaagc
     2581 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2641 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2701 ttcttagcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
     2761 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
     2821 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
     2881 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
     2941 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
     3001 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
     3061 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
     3121 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
     3181 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
     3241 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
     3301 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
     3361 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
     3421 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg
     3481 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
     3541 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
     3601 tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
     3661 gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccactgtc gactacgcca
     3721 tcatggcgac agcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca
     3781 ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc
     3841 gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg
     3901 tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct
     3961 tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac
     4021 gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca
     4081 tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc
     4141 cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg
     4201 gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc
     4261 ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca
     4321 caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc
     4381 tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg
     4441 atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga
     4501 tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt
     4561 gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc
     4621 tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca
     4681 tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta gaattgtcaa ttttaatcct
     4741 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     4801 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     4861 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     4921 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     4981 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     5041 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     5101 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     5161 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     5221 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     5281 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     5341 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     5401 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     5461 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     5521 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     5581 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     5641 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     5701 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     5761 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     5821 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     5881 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     5941 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     6001 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     6061 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     6121 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     6181 acttggtatt ccgaatcttg ccctgcacga ataccagctc cgcgaagtcg ctcttcttga
     6241 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
     6301 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
     6361 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
     6421 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
     6481 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
     6541 ccgacggcca gcaggtaggc ctacaggctc atgccggccg ccgccgcctt ttcctcaatc
     6601 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
     6661 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
     6721 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     6781 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
     6841 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     6901 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt
     6961 cgaccctttc cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     7021 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
     7081 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     7141 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     7201 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     7261 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     7321 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     7381 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     7441 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     7501 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     7561 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     7621 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagccaagct
     7681 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7741 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7801 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7861 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8761 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8821 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8881 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8941 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9001 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9061 attacgccaa gctagcttcc cgcttggtcg ggccgcttcg cgagggcccg ttgacgacaa
     9121 cggtgcgatg ggtcccggcc ccggtcaaga cgatgccaat acgttgcgac actacgcctt
     9181 ggcactttta gaattgcctt atcgtcctga taagaaatgt ccgaccagct aaagacatcg
     9241 cgtccaatca aagcctagaa aatataggcg aagggacgct aataagtctt tcataagacc
     9301 gcgcaaatct aaaaatatcc ttagattcac gatgcggcac ttcggatgac ttccgagcga
     9361 gcctggaacc tcagaaaaac gtctgagaga taccgcggat ctcacacagg aaatttc
//
