LOCUS       sequence-316501-        9429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018297
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2133..2153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2154..2177
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2203..2259
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2260..2277)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2260..2276)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2269..2291)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2485..2504)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2604..2626
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2664..2682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3468..4667
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4898..6046)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6318..6689)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6722..6831
                     /note="incP origin of transfer"
     rep_origin      6984..7615
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8078..8666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8567..8586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8820..8837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8954..8975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8990..8995,8996..9013,9014..9020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9028..9044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9033..9055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9052..9068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9052..9068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatggcgg
      361 ccctgacgct catcctcgcg gcggcagtgt cacccgccat cgcgcagcag cagggaagcc
      421 tgccggagct tctgaacctg caacgcgatg acgggcagtg ggtcatgccg gccaaggatt
      481 actcggcgac ccggtatagt tccctggacc agatcaacat cctcaacgtg gaccagctga
      541 aggtcgcctg gaccttcagc accggggttc tgcgcggcca cgaaggcgca ccgctcgtgg
      601 tcggctcgac catgtacctc atcacccctt tcccgaacat cgtctatgct ctgaacctta
      661 cgcagccggg cccggccgtc aagtggaagt tcgtgccgga cacagacccg cgctccatcg
      721 gcgtcgcctg ctgcgacgtg gtgaaccgcg gcctcacgta cgccgatggc aaggtcttct
      781 tcaacaccct cgatgcccac acgtatgccc tgaacgcgaa cacgggcgag gtcgtctggc
      841 aggttaagca aggcgacgtg gcccgcggcg agaccatcac catggccccg ctcgtggtca
      901 aggacaaggt catcacgggg atcagcggcg gcgagtttgg catacgcggc ttcctcacgg
      961 ccaacgacat ccacacgggc gagcaggtgt ggcgcgccta cacgaccggc ccggacagcg
     1021 aggtcctgat cggtgacgac ttcaagccct tctacgattc gctcaagggc aaagatctcg
     1081 gagtgacgac atggccggag gaccagtgga aaatcggcgg ctcggcgacc tggacgtggc
     1141 tgtcgtacga cccggagctg gacctcctgt accacggcgt cggcaacccc gggacctgga
     1201 acccggacct caggcccggc gataacaagt ggtcgatgac gatcttcgcg cgcgacccgg
     1261 atacgggcag cgcgcgttgg gcttaccaaa agacccccca cgaccagtgg gactacgacg
     1321 gcgtgaacga gaacatcctg gtcacgctga ccatcaatgg caagccccgc aaagtcctgg
     1381 ttaacttcga tcgcaacggc ttcgcctaca ccatcgaccg cgcgaccggc gaggtgctgg
     1441 tggcccagcc gtacgtgtat gtcaactggg ccagcggcgt ggatctcgag acgggcgtgc
     1501 cggcccgcaa tgcggacaag tcgacgaagc agggcgtcaa caccaccaat atctgcccgg
     1561 cggccatggg gggcaaggac cagcagccgg ccgcgttctc gccccgcacc ggcctcttct
     1621 acgtgccgac caacaacttg tgtatggatt atgagggcac cgaagtcgcg tacgtcgcgg
     1681 gcgtgccgta cgtgggggcc acggtcaaga tgtacgccgg cccggggggc aaccgcgggg
     1741 aattcgtggc gtgggacggc gcccgcggcc gcaaggtctg gggtatcaag gagaacttct
     1801 cggtctggtc tggcgtgctc gccacgggcg gcaacgtcgt cttctatggc acgatggatg
     1861 ggtggtttaa ggccgtgaac gccgtcaccg gcgaggtcct ctggaccttc aaggtcgggt
     1921 cgggcatcat cggcgcgccg atgaccttcc tgggcccgga tggcaagcag tacgtcgcgg
     1981 tgtactccgg catcggcggc tggtcggggc tgaccgtggc cggcgacctg agcctcgagg
     2041 atccgaccgc ggccctgggc gcggtgaacg ccttctcgga cctgggccgc tacaccaaca
     2101 agggcggcat gctctacgtc ttcagcctcg ccgaaaacct ctattttcag ggacatcatc
     2161 accatcacca tcaccactga cagctatgac catgattacg ccaagcttgc atgcctgcag
     2221 gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     2281 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     2341 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     2401 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2461 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     2521 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtatgctcc cggcatccgc
     2581 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2641 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2701 gataataatg gtttcttagc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     2761 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
     2821 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     2881 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     2941 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     3001 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     3061 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     3121 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     3181 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     3241 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     3301 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     3361 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     3421 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     3481 tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     3541 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     3601 atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg
     3661 cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg
     3721 tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg
     3781 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     3841 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     3901 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
     3961 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     4021 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     4081 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     4141 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     4201 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     4261 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     4321 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     4381 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     4441 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     4501 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     4561 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     4621 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     4681 aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc tagaattgtc
     4741 aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact
     4801 gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg
     4861 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     4921 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     4981 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     5041 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     5101 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     5161 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     5221 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     5281 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     5341 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     5401 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     5461 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     5521 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     5581 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     5641 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
     5701 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     5761 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg
     5821 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
     5881 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
     5941 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
     6001 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
     6061 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
     6121 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
     6181 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc tccgcgaagt
     6241 cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt
     6301 tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg
     6361 ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg
     6421 aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc
     6481 aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt
     6541 ttgtagccct ggccgacggc cagcaggtag gcctacaggc tcatgccggc cgccgccgcc
     6601 ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc
     6661 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     6721 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     6781 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     6841 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     6901 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     6961 gaaaagatcc gtcgaccctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     7021 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     7081 cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
     7141 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     7201 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     7261 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     7321 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     7381 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     7441 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     7501 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     7561 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc
     7621 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat
     7681 ggcagccaag ctgaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9061 gctatgacca tgattacgcc aagctagctt cccgcttggt cgggccgctt cgcgagggcc
     9121 cgttgacgac aacggtgcga tgggtcccgg ccccggtcaa gacgatgcca atacgttgcg
     9181 acactacgcc ttggcacttt tagaattgcc ttatcgtcct gataagaaat gtccgaccag
     9241 ctaaagacat cgcgtccaat caaagcctag aaaatatagg cgaagggacg ctaataagtc
     9301 tttcataaga ccgcgcaaat ctaaaaatat ccttagattc acgatgcggc acttcggatg
     9361 acttccgagc gagcctggaa cctcagaaaa acgtctgaga gataccgcgg atctcacaca
     9421 ggaaatttc
//
