LOCUS       sequence-316503-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018299
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2136..2156
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2157..2180
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2206..2262
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2263..2280)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2263..2279)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2294)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2488..2507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2607..2629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2667..2685)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3471..4670
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4901..6049)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6321..6692)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6725..6834
                     /note="incP origin of transfer"
     rep_origin      6987..7618
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8081..8669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8570..8589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8823..8840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8957..8978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8993..8998,8999..9016,9017..9023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9031..9047
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9036..9058
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9055..9071
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9055..9071
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcgcc
      361 ggtcgatctc agtggcgagc atcctgctgg tcgccgtcac cttcgcgatc gcctacgcga
      421 acccggagct gatccggctc cagaccgacg acaagcagtg ggtgatgccg ggaaaggact
      481 atagcgcgtc gcgattcagc acactgaccc agatcaccaa ggagaacgtg aaaaacctgc
      541 ggccggcctg gacgttttcc acgggcacgc tccgcggcca cgagggggca ccgctggttg
      601 tgggcgcaac catgtacgtc cacacctcct tccccaataa cgtcttcgcc ctcgatctga
      661 agggcccgga tgtggggcgc atcaagtgga agtacctgcc gaagcaggat ccctcggtgc
      721 aggcagtggc ctgctgcgac aacgtcaacc ggggcctgag ctacgcggac ggcaagatct
      781 ttctcagcca gctggacggc catctggtgg cgctggactc cgccacgggc cgcgaggtct
      841 ggaaggcgca gaacgccgac ccgaaggtcg gcgccacgat gacgtccgca ccccatgtca
      901 tgaaggacaa ggtcatcgtc ggcgtctcgg gcggggagtt cggggtccgg ggccacgtca
      961 ccgcatacga tatcaagacg ggccagcagg cctggcgcgc gtacagcatg ggtcccgacg
     1021 aggaggtgct gctggcggac gacttcaacg cggcgaaccc gcacttcggc cgccagaacc
     1081 aaggcgtggc cacatggcag ggcgatcagt ggaagacggg cggcgggacg acgtggggct
     1141 ggtacgcttt cgaccccagc ctcaatctga tgtactacgg caccggtaat ccgtcgacgt
     1201 ggaatccgtc gcagcgccca ggcgacaata agtggagcat gaccatcttc gcgagggacg
     1261 tggatacggg caaggcgaag tgggcctacc agatgacccc ctgggacgcg tgggactatg
     1321 atggggtcaa cgagatgatc ctggcggatc tgaccgtcaa ggggcgaccg gtcaaggccg
     1381 tcgtccattt cgaccgaaat gggttcgcct acaccctgga ccggaccaac ggcaccctgc
     1441 tcgtcgcgga aaagttcgac ccggacgtca actgggccac ccacatcgac aagcagaccg
     1501 gccgccccgt cgaggtcccg gccatgcgga cacaggccgg caagaacagc cagggggtct
     1561 gcccggccgc cctgggcacg aaggaccagc aaccggccgc ctacagcccc cgcaccaagc
     1621 tgttctacgt cccgaccaac catgtgtgca tggattacga gccgttcgag gtgaagtaca
     1681 cggccggcca gccgtacgtg ggggcgaccc tccgcatgtt tccgggcccc ggaggccaca
     1741 tgggcgcctt cattgcctgg gatgccgcaa acggcaagat cgtctggtcg aacaaggagc
     1801 agttcagcgc gtggtccgga gcgctggtta ccgtcagcga cgtggcgttc tatggaacgc
     1861 tcgaaggcta cctcaaggcc gtcgatgccc gcacggggaa ggaactgtgg aagttcaaga
     1921 cgccctctgg catcatcggc aacccgatga cctatatcgg acccgacggg aagcagtacg
     1981 tggcgatcct cagcggggtc ggcggctggg cggggatcgg tctggccgcg ggcctcgagg
     2041 gagacaccga gggcctcggc gccgtcggcg cgtacaaaaa actcgcggag tacacgaacc
     2101 tgggcggcgt gctgaccgtg ttcagcttgc ccgccgaaaa cctctatttt cagggacatc
     2161 atcaccatca ccatcaccac tgacagctat gaccatgatt acgccaagct tgcatgcctg
     2221 caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca
     2281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2401 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2461 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtatgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2701 catgataata atggtttctt agcacccttt ctcggtcctt caacgttcct gacaacgagc
     2761 ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga
     2821 ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca
     2881 acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca
     2941 acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc
     3001 tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg
     3061 ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca
     3121 ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc
     3181 tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag
     3241 taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct
     3301 acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt
     3361 gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta tcagtgataa
     3421 agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca
     3481 acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga
     3541 ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc
     3601 actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg
     3661 gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg gccggcgcca
     3721 ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat atcgggcgga
     3781 tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt gccgatatca
     3841 ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc gggttcggga
     3901 tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac gctccgttct
     3961 tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt
     4021 cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc gcttcgttcc
     4081 ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac
     4141 ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc tttcactggg
     4201 acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca ctcgcccagg
     4261 caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc atgctcggaa
     4321 tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga tggatggcgt
     4381 tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg caagcaatgt
     4441 tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg gcggcgctca
     4501 ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg gcttctataa
     4561 caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc tgcctgccgg
     4621 cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac
     4681 gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg ccctagaatt
     4741 gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat
     4801 actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc
     4861 tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
     4921 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
     4981 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
     5041 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
     5101 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
     5161 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
     5221 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
     5281 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
     5341 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
     5401 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
     5461 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
     5521 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
     5581 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
     5641 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
     5701 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
     5761 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
     5821 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
     5881 cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc
     5941 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
     6001 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
     6061 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
     6121 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
     6181 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agctccgcga
     6241 agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc
     6301 cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc
     6361 ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca
     6421 ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg
     6481 cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg
     6541 attttgtagc cctggccgac ggccagcagg taggcctaca ggctcatgcc ggccgccgcc
     6601 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
     6661 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     6721 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     6781 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     6841 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     6901 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     6961 gcggaaaaga tccgtcgacc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
     7021 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     7081 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     7141 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     7201 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     7261 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     7321 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     7381 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     7441 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     7501 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     7561 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     7621 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     7681 aatggcagcc aagctgacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7741 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7801 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7861 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7921 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8821 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8881 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8941 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9001 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9061 acagctatga ccatgattac gccaagctag cttcccgctt ggtcgggccg cttcgcgagg
     9121 gcccgttgac gacaacggtg cgatgggtcc cggccccggt caagacgatg ccaatacgtt
     9181 gcgacactac gccttggcac ttttagaatt gccttatcgt cctgataaga aatgtccgac
     9241 cagctaaaga catcgcgtcc aatcaaagcc tagaaaatat aggcgaaggg acgctaataa
     9301 gtctttcata agaccgcgca aatctaaaaa tatccttaga ttcacgatgc ggcacttcgg
     9361 atgacttccg agcgagcctg gaacctcaga aaaacgtctg agagataccg cggatctcac
     9421 acaggaaatt tc
//
