LOCUS       sequence-316504-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018301
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2142..2162
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2163..2186
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2212..2268
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2269..2286)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2269..2285)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2278..2300)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2494..2513)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2613..2635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2673..2691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3477..4676
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4907..6055)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6327..6698)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6731..6840
                     /note="incP origin of transfer"
     rep_origin      6993..7624
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8087..8675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8576..8595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8829..8846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8963..8984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8999..9004,9005..9022,9023..9029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9037..9053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9042..9064
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9061..9077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9061..9077
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accgtgatcg
      361 gccgctggcg tacgtgggcc atcgccgcgg tcaccgtcgc gctggtctcg gtcggcgccg
      421 cggccgacca aaagggcgag tggaccatga ccgggcgcac gtatgacctc cagcgcttct
      481 cgccgctgga ccagattacc gcttccaacg tcggaaccct cgtcccgacg tggaccttct
      541 cgacgggcgc ccttcgcggt catgagggca acccccttgt gatcggcaac gtcatgtacg
      601 tgcattccgc gttccccaac accgtctatg ccctggacct caccaaggcc ggcgctccca
      661 tgctttggaa gcacgtccct gcccagccgg cggagaccat cccggtcgcg tgctgcgacg
      721 tcgtgaaccg gggcctcgcg taccacccgt cgggcaagct cttcatcgag ctcctcgccg
      781 gggatctcct ggccctagac gcgaagacgg gcaagcagct gtggcgcgtg aagaatgcgg
      841 attacaagga gggatcgacc atgaccaacg ccccgatcgt catcaaagac atcgtcatcg
      901 ccggcatctc cgggggcgag ttcggcgtcc gggggcgcgt cacggccttc aacgtcaaca
      961 ccggccagga ggtgtggcgc gcctacagca cgggccccga ctcggaggtc aagatcggct
     1021 cggacttcat cgctaattac ccctcgcaca agggccccga cctgggcgtg accacctggc
     1081 agggtgagca gtggaagcgc ggcggcggca cgacctgggg gtggtactcc tacgacccgg
     1141 agctcaatct cttctactat gggacgggca accccggcac gtggaacccg gaccagcgcc
     1201 cgggcgacaa caagtggagc atgacgatct tcgcccggaa ccccgacacc ggcatggcgc
     1261 gctgggcgta ccagatgacc ccccatgacg agtgggacta tgacggcgtg aacgaaatga
     1321 tcctcgccga cctgaacgtc aacggcaaga agacgaaggc cctcgtgcac ttcgatcgca
     1381 acggattcgg gtacaccctc gatcggacca ccggcaaggt gctggtggcc gagcacttcg
     1441 gcccgggcgt caattgggca agcaaggtcg atctcacgac cggagtcccc gtacgcgaca
     1501 gcaagtacgg caccacgagc aaggagaata cgaccggcat ctgcccggcg gccatcggct
     1561 acaaggacca gcagcccgcc gccttctcgc cgaaaacggg ctttttctac gtccccacga
     1621 acaacatctg catggattat gagggcgtcg aggttaaata cgcggtgggc cagccgtacg
     1681 ttggggccat cgtgcggatg ttccccgggc ccggcggaaa tcgcgggcgc ttcatcgcct
     1741 gggacgcgac gaagggcaag gtcgtctggg agatcaaaga acagctcgca gcgtatggcg
     1801 gcgcactggc caccgccgga ggcgtcgtgt tctacggcac catggagggt tggctcaagg
     1861 gcgtcgacgc ccaatccggc aaggagctct ggaagttcaa gaccccctcg ggcatcatcg
     1921 gaaacccgat gacctatctg ggcccgaacg ggcgccagta cgtcgccgtc ctgtccggcg
     1981 tgggcgggtg ggcgggcatc ggcgtggctg caggcatcgg ccctgaggat cccactgcgg
     2041 gcctcggcgc gctcggcgcg ttcggtgacg cggacaaatt ctccacccag gggggcgtgc
     2101 tgacggtctt cggcctcccg gagaagctgg cctcccgggc cgaaaacctc tattttcagg
     2161 gacatcatca ccatcaccat caccactgac agctatgacc atgattacgc caagcttgca
     2221 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     2281 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2341 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2401 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     2461 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2521 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtatgctccc
     2581 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2641 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2701 taatgtcatg ataataatgg tttcttagca ccctttctcg gtccttcaac gttcctgaca
     2761 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     2821 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     2881 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     2941 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     3001 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     3061 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     3121 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     3181 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     3241 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     3301 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     3361 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     3421 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     3481 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     3541 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     3601 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg
     3661 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg
     3721 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg
     3781 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     3841 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     3901 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     3961 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     4021 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     4081 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     4141 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     4201 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     4261 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     4321 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     4381 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     4441 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     4501 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     4561 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     4621 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     4681 ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct
     4741 agaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg
     4801 agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta
     4861 aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat
     4921 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
     4981 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
     5041 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
     5101 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
     5161 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
     5221 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
     5281 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
     5341 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
     5401 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
     5461 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
     5521 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
     5581 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
     5641 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
     5701 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
     5761 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     5821 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     5881 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     5941 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     6001 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     6061 ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc
     6121 cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga
     6181 ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagct
     6241 ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc
     6301 cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc
     6361 atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg
     6421 gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc
     6481 aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg
     6541 cccgtgattt tgtagccctg gccgacggcc agcaggtagg cctacaggct catgccggcc
     6601 gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt
     6661 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
     6721 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     6781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     6841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     6901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     6961 tatgcagcgg aaaagatccg tcgacccttt ccgacgctca ccgggctggt tgccctcgcc
     7021 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     7081 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     7141 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     7201 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     7261 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     7321 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     7381 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     7441 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     7501 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     7561 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     7621 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     7681 cccaaaaatg gcagccaagc tgaccacttc tgcgctcggc ccttccggct ggctggttta
     7741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9061 caggaaacag ctatgaccat gattacgcca agctagcttc ccgcttggtc gggccgcttc
     9121 gcgagggccc gttgacgaca acggtgcgat gggtcccggc cccggtcaag acgatgccaa
     9181 tacgttgcga cactacgcct tggcactttt agaattgcct tatcgtcctg ataagaaatg
     9241 tccgaccagc taaagacatc gcgtccaatc aaagcctaga aaatataggc gaagggacgc
     9301 taataagtct ttcataagac cgcgcaaatc taaaaatatc cttagattca cgatgcggca
     9361 cttcggatga cttccgagcg agcctggaac ctcagaaaaa cgtctgagag ataccgcgga
     9421 tctcacacag gaaatttc
//
