LOCUS       sequence-316508-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018305
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2154..2174
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2175..2198
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2224..2280
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2281..2298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2281..2297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2290..2312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2506..2525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2625..2647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2685..2703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3489..4688
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4919..6067)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6339..6710)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6743..6852
                     /note="incP origin of transfer"
     rep_origin      7005..7636
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8099..8687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8588..8607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8841..8858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8975..8996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9011..9016,9017..9034,9035..9041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9049..9065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9054..9076
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9073..9089
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9073..9089
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcggc
      361 aggcacacct cctgggcctg atgctcgccc tgggcaccac cggggccctg gcgaacgagg
      421 acgtgctgaa gcgcacgcag gaccccaacc agcaggtcct ccagaccctg gattacgcta
      481 acacccgcta cagcaagctc gaccagatca acgcgtcgaa cgtgaagaac ctgcaggtgg
      541 cctggacctt ctcaaccggt gtgctccgcg gacatgaggg ctcgccgctg gttgtcggcg
      601 acatcatgta cgtgcatacg ccgttcccga acatcgtgta cgcgctggac ctgaacaacg
      661 acagcaagat tctgtggaag tacgagccga aacaggatcc ctcggtgatc ccggtgatgt
      721 gctgcgacac cgtcaaccgc ggcctggcct acgcggatgg cgccattatc ctgcatcagg
      781 ccgacacgac cctcgtctcc ctcgacgcta aaaccggcaa ggtgaactgg tcggtgaaga
      841 acggcgactc caaggtgggg gagaccaaca ccgccaccgt cctccccgtc aaggacaaga
      901 tcattgttgg gatctccgga gcggagtacg gcatccgggg ccatatgacg gcctacgacg
      961 cgaagaccgg caagcgagtc tggcgggcct atagcgtcgg gccggacgac gagatgctgg
     1021 tggaccccga aaagacgacg tcgctgggta agcccatcgg aaaggattcc tcgcttaaga
     1081 cctgggaggg cgaccagtgg aagaccggcg gcggtgcgac gtggggctgg tatagctacg
     1141 atccaaagct cgacctgttc tattatggca ccgcgaaccc gtcgacgtgg aacccgaagc
     1201 agcggccggg ggataataag tggacgatgg ccatcttcgc gcgcaacccg gacaccggtc
     1261 aggccaagtg gatctaccag atgacccccc atgacgaatg ggactacgac gggatcaacg
     1321 aaatgatcct caccgaccag aaggtagacg ggaaggagcg gcccctgctg acgcacttcg
     1381 accgaaacgg cttcgcctac acgcttgatc gcgcgaacgg cgaggtcctc gtcgccgaaa
     1441 agttcgaccc ggtggtgaac tgggcgagca aggtggatct cgataagggc tcgaagaact
     1501 acggccgccc cctggtcgtg tcgaagtact ccacggacca gaacggcgag gacgtgaact
     1561 cgaagggcat ctgcccggcc gcgctcggga ccaaggatca gcagccggcg gccttcagcc
     1621 cgaagaccca gcttttctac gtcccgacga accacgtgtg catggattac gagcccttca
     1681 aggtgaccta caccccgggc cagccgtacg tcggcgcgac gctgtcgatg tatcccgcgc
     1741 cgggcggaca tgggggcatg gggaacttca tcgcttggga caacatctcg ggcaagatca
     1801 agtggtcgaa cccggagcag tttagcgtct ggagcggcgc gctcgcgacc gccggcgacg
     1861 tggtgttcta cgggacgctg gagggttacc tgaaggcggt cgacagcaag acgggcaagg
     1921 aactgtacaa atttaagacc gcctcgggag ttatcggcaa cgtcatgacc tacacccaca
     1981 agggcaagca gtacgtcggg gttctctcgg gcgttggggg ctgggccggc atcggcctgg
     2041 cggccggcct gacggaccca aacgccggcc tcggggcagt ggggggatac gccgcgctct
     2101 cgcagtacac gaacctcggt ggccaactga cggtcttcgc ccttccgaac gccgaaaacc
     2161 tctattttca gggacatcat caccatcacc atcaccactg acagctatga ccatgattac
     2221 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
     2281 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2341 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2401 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     2461 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2521 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2581 ttgtatgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2641 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2701 atttttatag gttaatgtca tgataataat ggtttcttag caccctttct cggtccttca
     2761 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
     2821 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
     2881 gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc
     2941 tcctcaagca cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc
     3001 ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc
     3061 ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc
     3121 tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc
     3181 accgaatgcg tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc
     3241 ttgttcctga agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg
     3301 cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact
     3361 gtattcggct gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca
     3421 ccaacttatc agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga
     3481 ggccagacgt gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg
     3541 ctgtcggcat cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact
     3601 cgaacgacgt caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg
     3661 cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg
     3721 tctcgctggc cggcgccact gtcgactacg ccatcatggc gacagcgcct ttcctttggg
     3781 ttctctatat cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg
     3841 cttatattgc cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg
     3901 cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct
     3961 ccccccacgc tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct
     4021 gtttcctttt gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca
     4081 acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg
     4141 tcttcttcat catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg
     4201 aggatcgctt tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc
     4261 tgcattcact cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc
     4321 gggcactcat gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga
     4381 cacggggatg gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc
     4441 cggcgctgca agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag
     4501 gctcactggc ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga
     4561 tctatgcggc ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct
     4621 acttgctctg cctgccggcg ctgcgtcgcg ggctttggag cggcgcaggg caacgagccg
     4681 atcgctgatc gtggaaacga taggcctatg ccatgcgggt caaggcgact tccggcaagc
     4741 tatacgcgcc ctagaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg
     4801 ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct
     4861 gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct
     4921 agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc
     4981 gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg
     5041 atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga
     5101 aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt
     5161 tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac
     5221 gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca
     5281 ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt
     5341 cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt
     5401 agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct
     5461 gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt
     5521 ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc
     5581 gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc
     5641 acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca
     5701 gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc
     5761 gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg
     5821 cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg
     5881 gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga
     5941 cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat
     6001 cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc
     6061 gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt
     6121 cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt
     6181 cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca
     6241 cgaataccag ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa
     6301 tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg
     6361 accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg
     6421 gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc
     6481 agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca
     6541 tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggcctacagg
     6601 ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac
     6661 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
     6721 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     6781 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     6841 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     6901 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     6961 ccgaagcagg gttatgcagc ggaaaagatc cgtcgaccct ttccgacgct caccgggctg
     7021 gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg
     7081 tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg
     7141 gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg
     7201 gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg
     7261 caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg
     7321 gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg
     7381 cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt
     7441 gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc
     7501 caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac
     7561 cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga
     7621 accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc
     7681 gaacggcctc accccaaaaa tggcagccaa gctgaccact tctgcgctcg gcccttccgg
     7741 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7801 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7861 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7921 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7981 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8221 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8881 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8941 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9001 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9061 aacaatttca cacaggaaac agctatgacc atgattacgc caagctagct tcccgcttgg
     9121 tcgggccgct tcgcgagggc ccgttgacga caacggtgcg atgggtcccg gccccggtca
     9181 agacgatgcc aatacgttgc gacactacgc cttggcactt ttagaattgc cttatcgtcc
     9241 tgataagaaa tgtccgacca gctaaagaca tcgcgtccaa tcaaagccta gaaaatatag
     9301 gcgaagggac gctaataagt ctttcataag accgcgcaaa tctaaaaata tccttagatt
     9361 cacgatgcgg cacttcggat gacttccgag cgagcctgga acctcagaaa aacgtctgag
     9421 agataccgcg gatctcacac aggaaatttc
//
