LOCUS       sequence-316511-       10060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of GPR143-mScarlet-3xHA fusion protein for the 
            isolation of melanosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, 
            Lewis CA, Sabatini DM
  TITLE     MFSD12 mediates the import of cysteine into melanosomes and 
            lysosomes.
  JOURNAL   Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x.
  PUBMED    33208952
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x."
COMMENT     Sequence Label: MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)
FEATURES             Location/Qualifiers
     source          1..10060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5005..5031
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5044..5070
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5154..5271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5320..5830
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5749..5767
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5852..6451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5852..5871)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6348..6368
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6579..6812
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6884..7005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6921..6940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6975..6994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7045..7180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7200..7219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7201..7219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7229..7246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7229..7245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7238..7260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7387..7842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7474..7493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7684..7705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7869..7972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7973..8041,8042..8833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8191..8210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9004..9592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9493..9512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9746..9763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9880..9901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9916..9921,9922..9939,9940..9946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9954..9970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9959..9981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9978..9994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9978..9994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10013..10033
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10015..10033
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcccac catggcctcc
     3061 ccgcgcctag ggaccttctg ctgccccacg cgggacgcag ccacgcagct cgtgctgagc
     3121 ttccagccgc gggccttcca cgcgctctgc ctgggcagcg gcgggctccg cttggcgctg
     3181 ggccttctgc agctgctgcc cggccgccgg cccgcgggcc ccgggtcccc cgcgacgtcc
     3241 ccgccggcct cggtccgcat cctgcgcgct gccgctgcct gcgaccttct cggctgcctg
     3301 ggtatggtga tccggtccac cgtgtggtta ggattcccaa attttgttga cagcgtctcg
     3361 gatatgaacc acacggaaat ttggcctgct gctttctgcg tggggagtgc gatgtggatt
     3421 cagctgttgt acagtgcctg cttctggtgg ctgttttgct atgcagtgga tgcttatctg
     3481 gtgatccgga gatcggcagg actgagcacc atcctgctgt atcacatcat ggcgtggggc
     3541 ctggccaccc tgctctgtgt ggagggagcc gccatgctct actacccttc cgtgtccagg
     3601 tgtgagcggg gcctggacca cgccatcccc cactatgtca ccatgtacct gcccctgctg
     3661 ctggttctcg tggcgaaccc catcctgttc caaaagacag tgactgcagt ggcctcttta
     3721 cttaaaggaa gacaaggcat ttacacggag aacgagagga ggatgggagc cgtgatcaag
     3781 atccgatttt tcaaaatcat gctggtttta attatttgtt ggttgtcgaa tatcatcaat
     3841 gaaagccttt tattctatct tgagatgcaa acagatatca atggaggttc tttgaaacct
     3901 gtcagaactg cagccaagac cacatggttt attatgggaa tcctgaatcc agcccaggga
     3961 tttctcttgt ctttggcctt ctacggctgg acaggatgca gcctgggttt tcagtctccc
     4021 aggaaggaga tccagtggga atcactgacc acctcggctg ctgagggggc tcacccatcc
     4081 ccactgatgc cccatgaaaa ccctgcttcc gggaaggtgt ctcaagtggg tgggcagact
     4141 tctgacgaag ccctgagcat gctgtctgaa ggttctgatg ccagcacaat tgaaattcac
     4201 actgcaagtg aatcctgcaa caaaaatgag ggtgaccctg ctctcccaac ccatggagac
     4261 ctagcggccg ccggtagtgt cagcaagggc gaagcggtca tcaaagagtt catgaggttt
     4321 aaggtacata tggaagggtc aatgaatggg cacgaatttg agatagaagg tgaaggggaa
     4381 ggcaggccgt atgagggtac acaaaccgca aaattgaagg tcaccaaggg aggtccgctc
     4441 ccgttttcct gggacatact gagccctcag ttcatgtatg ggtctcgcgc cttcaccaag
     4501 cacccggcag acatacctga ttattataaa cagagcttcc ctgaagggtt taagtgggaa
     4561 cgcgtgatga actttgagga tgggggtgct gtaacagtaa ctcaggatac gtccctcgaa
     4621 gacgggacct tgatctacaa ggtcaaactt cgcgggacta attttccgcc cgatggacca
     4681 gtcatgcaaa agaaaacgat gggatgggag gcatcaacgg aaagactgta ccccgaagac
     4741 ggcgttctta aaggggacat taagatggcc cttaggctta aagatggggg gcgctatctc
     4801 gcggatttca aaaccaccta taaagctaag aaacccgttc aaatgccagg tgcgtataac
     4861 gtggacagga agctggacat aaccagccat aacgaggact atactgtcgt tgagcaatat
     4921 gagcgctcag aaggccgcca ttcaaccggt gggatggatg agctgtataa aggttctggg
     4981 agcgggagtg atgaattgta taagtaccct tacgatgtgc ccgactatgc tggaaccggg
     5041 tcatacccgt acgacgtacc tgattacgcc gggtcagcgg gctatccata tgatgtacct
     5101 gattactaat aagaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     5161 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     5221 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     5281 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     5341 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     5401 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     5461 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     5521 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     5581 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     5641 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     5701 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     5761 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     5821 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     5881 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5941 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6001 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6061 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6121 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6181 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6241 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6301 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6361 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6421 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     6481 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     6541 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6601 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6781 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6841 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7021 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7141 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7201 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7261 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7321 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7381 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7441 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7561 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7621 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7681 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7741 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7801 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7861 aaatgtgtgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7921 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7981 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8041 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8101 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8161 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8221 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8281 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8341 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8401 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8461 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8521 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8581 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8641 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8701 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8761 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8821 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8881 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8941 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9901 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9961 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    10021 ccctcactaa agggaacaaa agctggagct gcaagcttaa
//
