LOCUS       sequence-316512-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018315
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2157..2177
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2178..2201
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2227..2283
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2284..2301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2284..2300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2293..2315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2509..2528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2628..2650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2688..2706)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3492..4691
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4922..6070)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6342..6713)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6746..6855
                     /note="incP origin of transfer"
     rep_origin      7008..7639
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8102..8690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8591..8610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8844..8861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8978..8999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9014..9019,9020..9037,9038..9044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9052..9068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9057..9079
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9076..9092
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9076..9092
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaaac
      361 tccggaccgt ctccctggcc atcctcgcct tgatcatgtc ggggatggag ctggccgcgg
      421 ccgacgaact cctcgagatg cagaagaact cggataactg gatcatgccc gctgggacgt
      481 acgacaatca gcggtactcc aagctcgacc agatcaacac cgataacgcg caggatttgc
      541 gggtggcctg gacgttcagc accggagtcc tgcgcgggca cgagggcaac ccgctcgtga
      601 tcgacggcac catgtacgtc cacaccccgt tcccgaacaa ggtcttcgcc ctcgacctgg
      661 acgaggacgg cgccataaaa tggaagtacg agccggtgca gaactatgag gagaccgtcc
      721 cggtcatgtg ctgcgacacg atcaaccgcg gcctcgccta tggtgacggc aagatcttcc
      781 tggcgcaggc cgacaccacg cttgtcgcgc tcgatgccaa gaccggcaag gtcgtgtggt
      841 cggtcaagcg ggacgatccg aagcagggtg ccacgagcac cggggccccc cacgtcttta
      901 aggacaaggt cctcgtcggg atctcgggcg gtgagttcgg cgtccggggc tacctggaag
      961 cctacgacgt gaactcgggc gagcggatct ggaaggccta ctccaccggc cccgacgagg
     1021 aaatgctcat cgacccggaa aagaccacgc acctcctcaa gccggtcgga aagaactcgt
     1081 ccctcgagag ctgggagggc gagcagtgga agatcggcgg cggcacgacc tggggctggt
     1141 ttgcgtacga ccccgagctc aacctcgtgt actacggcac ggccaacccc ggcacctgga
     1201 acccggtgca gcgccccggc gacaatcgct ggtcgatgac catattcgcg cgcgacctcg
     1261 acaccggagt tgccaaatgg gcgtatcaga tgaccccgca cgacgagtgg gactacgacg
     1321 gcgtcaacga gatggtgctc gtggaccaga agatcgacgg cgaggagagg aagctcctgg
     1381 tccacctgga ccgcaacggg tttggctaca cgctcgaccg tgtcaccggg gagctcctgg
     1441 tggctgagaa gttcgacccg gtggtcaact gggcgaccca cgtggacatg gagaccggcc
     1501 gcccccaggt cgacccgcgc tactcaaccc accagaacgg ccaggatgtg aataccacga
     1561 acatctgccc cgcagcgctc ggctccaagg accagcagcc ggttgccttc agtccgcgga
     1621 ccggcctctt ctacgtccct gcgaatcacg tctgcatgaa ctatgaaccg ttcgaagtga
     1681 gctataccgc cggccaaccc tacgtcggtg cgacgcttac catgttcccg ggcggcaagg
     1741 tgtcgggcga tgggtcgacc cacctgggcc gcttcttcgc ctgggacgcg cggaagggca
     1801 aggtggtgtg ggaaatcccg gagcgcttct ctgtctggag cggcaccctc gccaccgcgg
     1861 gcgatatcgt cgcgtatggg accctggaag gctacctcaa agtcgtcgac gcgaagagcg
     1921 ggcgcgagct ttatcgattt aagaccccca gcggcatcat cggcaacgtg atgacgtacc
     1981 agcacaaggg taagcagtac ctcgccgtgc tgtccggcct gggcggctgg gcgggcatcg
     2041 gtatggccgc tgggctccag ggggagaccg agggcctcgg ggcggtcggg gcctaccgga
     2101 gcctggagga ttggaccaac ctggggggca cccttaccgt cttcaccctt cccgccgaaa
     2161 acctctattt tcagggacat catcaccatc accatcacca ctgacagcta tgaccatgat
     2221 tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     2281 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     2341 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     2401 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     2461 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     2521 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2581 ggcttgtatg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2701 cctattttta taggttaatg tcatgataat aatggtttct tagcaccctt tctcggtcct
     2761 tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc
     2821 tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc
     2881 ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc
     2941 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
     3001 tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc
     3061 tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc
     3121 gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc
     3181 ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc
     3241 cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt
     3301 ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc
     3361 actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt
     3421 ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc
     3481 ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat tctgagcact gtcgcgctcg
     3541 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
     3601 actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttggtgcaat
     3661 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc
     3721 tcgtctcgct ggccggcgcc actgtcgact acgccatcat ggcgacagcg cctttccttt
     3781 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
     3841 gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga
     3901 gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt
     3961 tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg
     4021 gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc
     4081 tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg
     4141 cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg
     4201 gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca
     4261 ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa
     4321 ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg
     4381 cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa
     4441 tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc
     4501 aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg
     4561 cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc
     4621 tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag
     4681 ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg acttccggca
     4741 agctatacgc gccctagaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc
     4801 gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt
     4861 cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc
     4921 cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc
     4981 ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat
     5041 ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc
     5101 tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga
     5161 atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc
     5221 aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt
     5281 ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc
     5341 attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct
     5401 cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct
     5461 gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca
     5521 cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt
     5581 tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg
     5641 gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt
     5701 tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt
     5761 ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg
     5821 ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag
     5881 ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc
     5941 cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg
     6001 catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg
     6061 tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt
     6121 attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga
     6181 agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct
     6241 gcacgaatac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg
     6301 caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg
     6361 cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc
     6421 agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt
     6481 ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc
     6541 tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggcctac
     6601 aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag
     6661 tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg
     6721 ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc
     6781 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     6841 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     6901 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     6961 accccgaagc agggttatgc agcggaaaag atccgtcgac cctttccgac gctcaccggg
     7021 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
     7081 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
     7141 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     7201 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     7261 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     7321 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     7381 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     7441 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     7501 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     7561 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     7621 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
     7681 cgcgaacggc ctcaccccaa aaatggcagc caagctgacc acttctgcgc tcggcccttc
     7741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8221 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8461 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9001 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9061 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta gcttcccgct
     9121 tggtcgggcc gcttcgcgag ggcccgttga cgacaacggt gcgatgggtc ccggccccgg
     9181 tcaagacgat gccaatacgt tgcgacacta cgccttggca cttttagaat tgccttatcg
     9241 tcctgataag aaatgtccga ccagctaaag acatcgcgtc caatcaaagc ctagaaaata
     9301 taggcgaagg gacgctaata agtctttcat aagaccgcgc aaatctaaaa atatccttag
     9361 attcacgatg cggcacttcg gatgacttcc gagcgagcct ggaacctcag aaaaacgtct
     9421 gagagatacc gcggatctca cacaggaaat ttc
//
