LOCUS       sequence-316523-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HPV18 E7 D10K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Hatterschide J, Brantly AC, Grace M, Munger K, White EA
  TITLE     A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 
            Mediates Binding to PTPN14 and Repression of Epithelial 
            Differentiation.
  JOURNAL   J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 
            10.1128/JVI.01024-20. Print 2020 Aug 17.
  PUBMED    32581101
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2020-08-17"; volume: "94(17). pii: JVI.01024-20. doi"; pages:
            " 10.1128/JVI.01024-20. Print 2020 Aug 17"
COMMENT     Sequence Label: p8141 MSCV-P C-FlagHA 18E7 D10K
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..28
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(444..468)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             500..523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             539..565
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             539..565
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(613..636)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gccskkawct cctttatcca gccctcactc cttctctagg cgccggaatt agatctaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agttggcacc atgcatggac ctaaggcaac attgcaaaaa attgtattgc atttagagcc
      181 ccaaaatgaa attccggttg accttctatg tcacgagcaa ttaagcgact cagaggaaga
      241 aaacgatgaa atagatggag ttaatcatca acatttacca gcccgacgag ccgaaccaca
      301 acgtcacaca atgttgtgta tgtgttgtaa gtgtgaagcc agaattgagc tagtagtaga
      361 aagctcagca gacgaccttc gagcattcca gcagctgttt ctgaacaccc tgtcctttgt
      421 gtgtccgtgg tgtgcatccc agcagccaac tttcttgtac aaagtggttg atctagaggg
      481 cccgcggttc gaactcgagg actacaagga cgacgatgac aagtcggccg ctggaggata
      541 cccctacgac gtgcccgact acgcctagaa ttctaccggg taggggaggc gcttttccca
      601 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      661 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      721 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      781 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      841 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      901 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggct
//
