LOCUS       sequence-316524-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HPV18 E7 R84S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Hatterschide J, Brantly AC, Grace M, Munger K, White EA
  TITLE     A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 
            Mediates Binding to PTPN14 and Repression of Epithelial 
            Differentiation.
  JOURNAL   J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 
            10.1128/JVI.01024-20. Print 2020 Aug 17.
  PUBMED    32581101
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2020-08-17"; volume: "94(17). pii: JVI.01024-20. doi"; pages:
            " 10.1128/JVI.01024-20. Print 2020 Aug 17"
COMMENT     Sequence Label: p8193 MSCV-P C-FlagHA 18E7 R84S
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(441..465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             497..520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             536..562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             536..562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(610..633)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1006..1025
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ccsktatctc ctttatcagc ctcactcctt ctctaggcgc cggaattaga tctaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagt
      121 tggcaccatg catggaccta aggcaacatt gcaagacatt gtattgcatt tagagcccca
      181 aaatgaaatt ccggttgacc ttctatgtca cgagcaatta agcgactcag aggaagaaaa
      241 cgatgaaata gatggagtta atcatcaaca tttaccagcc cgacgagccg aaccacaacg
      301 tcacacaatg ttgtgtatgt gttgtaagtg tgaagccaga attgagctag tagtagaaag
      361 ctcagcagac gaccttagcg cattccagca gctgtttctg aacaccctgt cctttgtgtg
      421 tccgtggtgt gcatcccagc agccaacttt cttgtacaaa gtggttgatc tagagggccc
      481 gcggttcgaa ctcgaggact acaaggacga cgatgacaag tcggccgctg gaggataccc
      541 ctacgacgtg cccgactacg cctagaattc taccgggtag gggaggcgct tttcccaagg
      601 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      661 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      721 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      781 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      841 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      901 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcacgggc
      961 gggctcacgg gcggggcggg cgcccgaagt cctccggagg cccggcattc tgcacgcttc
     1021 aaaagcgcac gtctgccgcg ctgtctctct tctcatctcc ggaccgttcg actgcagcca
     1081 agccttacca tggaccggag attamaaggt cag
//
