LOCUS       sequence-316525-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HPV31 E7 R77S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Hatterschide J, Brantly AC, Grace M, Munger K, White EA
  TITLE     A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 
            Mediates Binding to PTPN14 and Repression of Epithelial 
            Differentiation.
  JOURNAL   J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 
            10.1128/JVI.01024-20. Print 2020 Aug 17.
  PUBMED    32581101
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2020-08-17"; volume: "94(17). pii: JVI.01024-20. doi"; pages:
            " 10.1128/JVI.01024-20. Print 2020 Aug 17"
COMMENT     Sequence Label: p8194 MSCV-P C-FlagHA 31E7 R77S
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(389..413)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             445..468
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             484..510
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             484..510
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(558..581)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tctaggcgcc ggaattagat ctaatacgac tcactatagg gagacccaag ctggctagtt
       61 aagctatcaa caagtttgta caaaaaagtt ggcaccatgc gtggagaaac acctacgttg
      121 caagactatg tgttagattt gcaacctgag gcaactgacc tccactgtta tgagcaatta
      181 cccgacagct cagatgagga ggatgtcata gacagtccag ctggacaagc agaaccggac
      241 acatccaatt acaatatcgt taccttttgt tgtcagtgta agtctacact tcgtttgtgt
      301 gtacagagca cacaagtaga tattagcata ttgcaagagc tgttaatggg ctcatttgga
      361 atcgtgtgcc ccaactgttc tactagactg ccaactttct tgtacaaagt ggttgatcta
      421 gagggcccgc ggttcgaact cgaggactac aaggacgacg atgacaagtc ggccgctgga
      481 ggatacccct acgacgtgcc cgactacgcc tagaattcta ccgggtaggg gaggcgcttt
      541 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
      601 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
      661 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
      721 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
      781 agatggacag caccgctgag caatggaa
//
