LOCUS       sequence-316526-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HPV6 E7 R77S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Hatterschide J, Brantly AC, Grace M, Munger K, White EA
  TITLE     A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 
            Mediates Binding to PTPN14 and Repression of Epithelial 
            Differentiation.
  JOURNAL   J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 
            10.1128/JVI.01024-20. Print 2020 Aug 17.
  PUBMED    32581101
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2020-08-17"; volume: "94(17). pii: JVI.01024-20. doi"; pages:
            " 10.1128/JVI.01024-20. Print 2020 Aug 17"
COMMENT     Sequence Label: p8195 MSCV-P C-FlagHA 6E7 R77S
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(399..423)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             455..478
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             494..520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             494..520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(568..591)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctaatac gactcactat agggagaccc
       61 aagctggcta gttaagctat caacaagttt gtacaaaaaa gttggcatgc atggaagaca
      121 tgttacccta aaggatattg tattagacct gcaacctcca gaccctgtag ggttacattg
      181 ctatgagcaa ttagtagaca gctcagaaga tgaggtggac gaagtggacg gacaagattc
      241 acaaccttta aaacaacatt tccaaatagt gacctgttgc tgtggatgtg acagcaacgt
      301 tcgactggtt gtgcagtgta cagaaacaga catcagcgaa gtgcaacagc ttctgttggg
      361 aacactaaac atagtgtgtc ccatctgcgc accgaagacc ccaactttct tgtacaaagt
      421 ggttgatcta gagggcccgc ggttcgaact cgaggactac aaggacgacg atgacaagtc
      481 ggccgctgga ggatacccct acgacgtgcc cgactacgcc tagaattcta ccgggtaggg
      541 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      601 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      661 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      721 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      781 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggg
//
