LOCUS       sequence-316527-        3571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gentamycin resistant polycistronic host vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 64 pCOLI_G2
FEATURES             Location/Qualifiers
     source          1..3571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..268)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      264..311
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        378..469
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1334..1373
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1404..1992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1893..1912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2053..2586)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2775..2780,2781..2797,2798..2803))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     2897..2914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2929..3068
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2994..3013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3154..3176
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3170..3361)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctctaga aggcctagat cttgtaaaac gacggccagt gaattccccg ggaagcttcg
      121 ccagggtttt cccagtcgag ctcgatatcg gtaccagcgg ataacaattt cacatccgga
      181 ttcgcgaacg cgtctcgaga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      241 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      301 ggggtttttt gctgaaagga ggaactatat ccgactagta gcccgcctaa tgagcgggct
      361 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      541 cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac gagtgggata
      601 catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg aagagcgttt
      661 tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc gtgtagatgc
      721 ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg ttgaatactc
      781 cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat gtagtgccgc
      841 cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa
      901 ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga
      961 accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat
     1021 ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca
     1081 attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc
     1141 tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat
     1201 tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag
     1261 tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa
     1321 gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg
     1381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2041 tttgctcaca tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     2101 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     2161 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     2221 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataaa tctcactacg
     2281 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     2341 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     2401 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     2461 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     2521 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
     2581 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     2641 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     2701 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     2761 gctacttgca ttacagttta cgaaccgaac aggcttatgt caattctttc ctgcgttatc
     2821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2941 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     3001 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3061 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3121 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3181 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     3241 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     3301 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     3361 ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta atgataccga tgaaacgaga
     3421 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     3481 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     3541 ccagcgcttc gttaatacag atgtaggtgt t
//
