LOCUS       sequence-316530-        5294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 3C cleavable His-Myc tag for insect cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 60 pLIB_HIS_MYC
FEATURES             Location/Qualifiers
     source          1..5294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(307..1098,1099..1167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     930..949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1168..1272)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1298..1752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1435..1456)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1646..1665
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  1936..2101
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    2211..2345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2235..2254)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2289..2308
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2574..2597)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2616..2645)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2652..2681)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2688..2717)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2724..2753)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2760..2789)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2796..2825)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2838..2867)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(2841..2870)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(2844..2873)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(2928..3019)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2986..3003)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     protein_bind    3067..3100
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(3496..3501,3502..3518,3519..3524)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3713..4246
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSIGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4313..4537
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(4671..4688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4842..5294,1..136))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4922..4941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
       61 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      421 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
      661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      721 aaaagcggtt aattccttcg gtccaccgat ggttgtcaga agtaagttgg ccgcagtgtt
      781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      901 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1201 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1261 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     1321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     1381 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     1441 tggaacaaga gtccactatt aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1501 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1561 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1621 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1681 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1741 tacagggcgc gtcccattcg ccattcaggc tgcaaataag cgttgatatt cagtcaatta
     1801 caaacattaa taacgaagag atgacagaaa aattttcatt ctgtgacaga gaaaaagtag
     1861 ccgaagatga cggtttgtca catggagttg gcaggatgtt tgattaaaaa cataacagga
     1921 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1981 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     2041 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     2101 tcggatctcc taggggttat gatagttatt gctcagcggt ggcagcagcc aactcagctt
     2161 cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca gatccagaca
     2221 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2281 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2341 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2401 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagtac
     2461 ttctcgacaa gcttgtcgag actgcaggct ctagattcga aagcggccgc gactagtgag
     2521 ctcgtcgacg taggcctttg aattccgcgc gctcatatgt cggaccggga tccgggcccc
     2581 tggaacagaa cttccagact accactcgac ccgttcaaat cttcttccga aataagtttc
     2641 tgttctccgt tcagatcctc ctcagagatt aacttctgtt cgccgttcaa atcttcctct
     2701 gagatcagtt tttgttcgcc attcaagtct tcttcggaga ttaacttttg ctcaccattc
     2761 agatcttcct ccgagatcaa cttctgctcc ccatttaagt cttcctcaga gataagtttc
     2821 tgctcggagc ccgaactgtg atgatgatga tgatggtgat gatgatgatg atggctgctg
     2881 cccatagatc cgcgcccgat ggtgggacgg tatgaataat ccggaatatt tataggtttt
     2941 tttattacaa aactgttacg aaaacagtaa aatacttatt tatttgcgag atggttatca
     3001 ttttaattat ctccatgatc tattaatatt ccggagtagg tcgcgaatcg atactagtaa
     3061 aaccagataa cttcgtataa tgtatgctat acgaagttat ggtaccgcgg ccgcgtagag
     3121 gatctgttga tcagcagttc aacctgttga tagtacaaac aaagctgtag ccaaccacta
     3181 gaactatagc tagagtcctg ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg
     3241 cgaaccactt catccggggt cagcaccacc ggcaagcgcc gcgacggccg aggtctaccg
     3301 atctcctgaa gcaagggcag atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc
     3361 gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga
     3421 aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     3481 aaggcacaaa cccagttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt
     3541 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3601 gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg ggcatccaag
     3661 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3721 cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag
     3781 tattggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc
     3841 tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga
     3901 ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga
     3961 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg
     4021 tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc agggcattgc
     4081 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta
     4141 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg
     4201 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc
     4261 cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacgcc gcgaatatag
     4321 tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa
     4381 aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg
     4441 actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc
     4501 tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt
     4561 tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtctatgcg gtgtgaaata
     4621 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     4681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5281 agaatagtat ttgg
//
