LOCUS       sequence-316539-         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the ZNF593 transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0226
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      529..538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(581..601)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttgtaatcca gaggttgatt gtcgacttaa cgcgtgtcct ttaattgaaa tggcctcggt
       61 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggactag
      121 ttcattacta ggtagaggta tccatctcag ggacctcagt ggacacttcc gtgggcactg
      181 ccagccgcct ggggggcaca taggatccca tacccgctgc cctctccgcc tcttcctgac
      241 tgtagggctc gacgctcagc tgcttcagcc ttttcttgtg gtctttggat cggaagtggg
      301 tcttcaggtt ggtggaatcg atgaagtacc tcgcgcaggc cagacagcgg tgcagaccgc
      361 cccctggcag gtcggggtcg aactcggcgt ttgggtcggg ctggggtcgt gcggatccct
      421 gaggccgcag ctcgcggtga atctcatcca agtccggccg ccgccgcttc gccttcatct
      481 gccgggctag agagtgcgct cggtgcgcgc ctgtccggcg ggagcgaccc atggtggcgg
      541 tgctagcctc acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg
      601 agaatgaac
//
