LOCUS       sequence-316550-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as
            a control with MelanoTag-PURO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, 
            Lewis CA, Sabatini DM
  TITLE     MFSD12 mediates the import of cysteine into melanosomes and 
            lysosomes.
  JOURNAL   Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x.
  PUBMED    33208952
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x."
COMMENT     Sequence Label: MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-...
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4990..5019
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    5064..5181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5230..5740
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5659..5677
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5762..6361
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5762..5781)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6258..6278
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6489..6722
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6794..6915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6831..6850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6885..6904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6955..7090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7110..7129)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7111..7129)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7139..7156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7139..7155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7148..7170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7297..7752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7384..7403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7594..7615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7779..7882
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7883..7951,7952..8743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8101..8120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8914..9502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9403..9422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9656..9673
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9790..9811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9826..9831,9832..9849,9850..9856)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9864..9880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9869..9891
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9888..9904
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9888..9904
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9923..9943
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9925..9943
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcccac catggcctcc
     3061 ccgcgcctag ggaccttctg ctgccccacg cgggacgcag ccacgcagct cgtgctgagc
     3121 ttccagccgc gggccttcca cgcgctctgc ctgggcagcg gcgggctccg cttggcgctg
     3181 ggccttctgc agctgctgcc cggccgccgg cccgcgggcc ccgggtcccc cgcgacgtcc
     3241 ccgccggcct cggtccgcat cctgcgcgct gccgctgcct gcgaccttct cggctgcctg
     3301 ggtatggtga tccggtccac cgtgtggtta ggattcccaa attttgttga cagcgtctcg
     3361 gatatgaacc acacggaaat ttggcctgct gctttctgcg tggggagtgc gatgtggatt
     3421 cagctgttgt acagtgcctg cttctggtgg ctgttttgct atgcagtgga tgcttatctg
     3481 gtgatccgga gatcggcagg actgagcacc atcctgctgt atcacatcat ggcgtggggc
     3541 ctggccaccc tgctctgtgt ggagggagcc gccatgctct actacccttc cgtgtccagg
     3601 tgtgagcggg gcctggacca cgccatcccc cactatgtca ccatgtacct gcccctgctg
     3661 ctggttctcg tggcgaaccc catcctgttc caaaagacag tgactgcagt ggcctcttta
     3721 cttaaaggaa gacaaggcat ttacacggag aacgagagga ggatgggagc cgtgatcaag
     3781 atccgatttt tcaaaatcat gctggtttta attatttgtt ggttgtcgaa tatcatcaat
     3841 gaaagccttt tattctatct tgagatgcaa acagatatca atggaggttc tttgaaacct
     3901 gtcagaactg cagccaagac cacatggttt attatgggaa tcctgaatcc agcccaggga
     3961 tttctcttgt ctttggcctt ctacggctgg acaggatgca gcctgggttt tcagtctccc
     4021 aggaaggaga tccagtggga atcactgacc acctcggctg ctgagggggc tcacccatcc
     4081 ccactgatgc cccatgaaaa ccctgcttcc gggaaggtgt ctcaagtggg tgggcagact
     4141 tctgacgaag ccctgagcat gctgtctgaa ggttctgatg ccagcacaat tgaaattcac
     4201 actgcaagtg aatcctgcaa caaaaatgag ggtgaccctg ctctcccaac ccatggagac
     4261 ctagcggccg ccggtagtgt cagcaagggc gaagcggtca tcaaagagtt catgaggttt
     4321 aaggtacata tggaagggtc aatgaatggg cacgaatttg agatagaagg tgaaggggaa
     4381 ggcaggccgt atgagggtac acaaaccgca aaattgaagg tcaccaaggg aggtccgctc
     4441 ccgttttcct gggacatact gagccctcag ttcatgtatg ggtctcgcgc cttcaccaag
     4501 cacccggcag acatacctga ttattataaa cagagcttcc ctgaagggtt taagtgggaa
     4561 cgcgtgatga actttgagga tgggggtgct gtaacagtaa ctcaggatac gtccctcgaa
     4621 gacgggacct tgatctacaa ggtcaaactt cgcgggacta attttccgcc cgatggacca
     4681 gtcatgcaaa agaaaacgat gggatgggag gcatcaacgg aaagactgta ccccgaagac
     4741 ggcgttctta aaggggacat taagatggcc cttaggctta aagatggggg gcgctatctc
     4801 gcggatttca aaaccaccta taaagctaag aaacccgttc aaatgccagg tgcgtataac
     4861 gtggacagga agctggacat aaccagccat aacgaggact atactgtcgt tgagcaatat
     4921 gagcgctcag aaggccgcca ttcaaccggt gggatggatg agctgtataa aggttctggg
     4981 agcgggagtg aacaaaaact catctcagaa gaggatctgt aagaattctc gacctcgaga
     5041 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     5101 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     5161 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     5221 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     5281 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     5341 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     5401 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     5461 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     5521 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     5581 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     5641 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     5701 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac
     5761 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5821 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5881 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5941 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6001 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6061 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6121 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6181 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6241 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6301 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6361 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt
     6421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6481 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6541 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6601 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6661 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6721 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6781 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6901 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     6961 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7021 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7081 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7141 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7201 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7261 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7741 cgcttacaat ttaggtggca cttttcgggg aaatgtgtgc ggaaccccta tttgtttatt
     7801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8041 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8101 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8161 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8221 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8281 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8341 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8401 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8461 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8521 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8581 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8641 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8701 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9601 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9661 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9721 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9781 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9841 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9901 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     9961 gcaagcttaa
//
