LOCUS       sequence-316555-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018323
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2160..2180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2181..2204
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2230..2286
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2287..2304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2287..2303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2296..2318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2512..2531)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2631..2653
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2691..2709)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3495..4694
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4925..6073)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6345..6716)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6749..6858
                     /note="incP origin of transfer"
     rep_origin      7011..7642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8105..8693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8594..8613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8847..8864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8981..9002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9017..9022,9023..9040,9041..9047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9055..9071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9060..9082
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9079..9095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9079..9095
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcgcc
      361 tgcatatgcg cggcgcgcgg ggcatgttgc tcgtaggagg catgttgctc catctcacga
      421 tgaccagctc cgtggccagc gcaaacgagg agcttatccg cctggcgaag gacgagcgca
      481 attgggtgat gcagaccaag gactatacag tgcagcgctt cagcaagctg gaccagatcg
      541 acacgtcgaa cgtgaagaac ctccagccgg cgtggtcgtt ctccaccggc gtcctgcatg
      601 gccacgaggg cggcccgctc gtcgtcaatg gaatcatgta cgtgcactcg gcgtatccca
      661 ataacgtcta cgccatcgac ctggagcacc ccgagaagat tatgtgggag tatcgcccga
      721 aacagaaccc cgccgcccgc gcggtcgcgt gctgcgacct ggtcaaccgc ggcctcgcct
      781 acggagacgg gaagctgatc ctgagcacgc tcgacggcca gctcatcgcg atggacgcga
      841 agacgggaaa ggtcatctgg aacgtcgaga cggcggatgt cagcgtcggg caaacccaga
      901 cgcaggcgcc cttcgtcgtc aaggacaaag tcatcatcgg cagcgccggc gccgaactgg
      961 gcgtccgggg gtacgtcacg gcttttaacc tcaaagacgg caagaaggtc tggcgggcgt
     1021 tcgccaccgg ccccgataag gacatcctcc tggataagga cttcaacaag cagaaccccc
     1081 attatggccg cttcggcctc gggctcaaga cgtgggaggg cgacgcgtgg aaaatcggcg
     1141 gcgggacgaa ctggggctgg tacgcgtacg atccgaagct cgatcttatt tactatggta
     1201 gcggcaaccc cgccccgtgg aacgaaacga tgcgcccggg cgacaacaag tggaccatga
     1261 cgatgtgggg acggaacccc gacacgggac tggcgaagta tggctatcag aagacgccgc
     1321 atgacgagtg ggactacgcg ggcatcaact acatgatgct cacggaccaa gtgattaatg
     1381 gcaagatgac gcccctcgtc acgcatccgg accgtaacgg catcatctac accctcaacc
     1441 gagagacggg cagcctggtc gccgccgata agatcgaccc gtcggtcaat tggacccgta
     1501 aggtcgacct gaagaccggc atcgccatcc gggaccccga gttcagcacc cgcatggacc
     1561 acaaggcgcg cggcatctgt ccctccgcca tgggctacca taaccagggg ctggacagct
     1621 acgacccgcg ctcggaaatc ttcttcatgg gcgtgaacca tatctgcatg gactgggagc
     1681 cgttcatgct cccgtaccgc gcgggtcagt tcttcgttgg ggcgaccctc tccatgtatc
     1741 cgggcccgaa gggctacctg ggcgaggtta aggcttacga cgtcaaaacc aactctatga
     1801 agtgggagaa ggccgagaag ttctccgtct ggggcggcac catggcgacg gcgggcaacc
     1861 tcgtcttcta cggcacccta gacggctggt tcaaggctct ggacagtggc agcggcgagg
     1921 agctatggaa attcaagctg ccgtcgggcg tcatcggcca tccaattacc tacgagcata
     1981 atgggaagca gtatgtcgcg atcctctacg gcgtcggcgg ctggccgggc gtcggcctgg
     2041 tgttcgacct gaacgacccg acggcgggcc tgggcagcgt cggcgcgttc aaggagctcg
     2101 ccaaatacac gaagatgggc ggcggcctta tggtctttgc gctgccggag ggccgcgccg
     2161 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ctatgaccat
     2221 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     2281 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2341 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2401 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     2461 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     2521 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     2581 acgggcttgt atgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     2641 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     2701 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcacc ctttctcggt
     2761 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     2821 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     2881 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     2941 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     3001 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     3061 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     3121 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     3181 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     3241 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     3301 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     3361 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     3421 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     3481 tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc
     3541 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     3601 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc
     3661 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct
     3721 tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca gcgcctttcc
     3781 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     3841 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     3901 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     3961 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     4021 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     4081 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     4141 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     4201 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     4261 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     4321 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     4381 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     4441 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     4501 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     4561 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     4621 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     4681 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     4741 gcaagctata cgcgccctag aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     4801 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
     4861 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
     4921 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     4981 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     5041 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     5101 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     5161 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     5221 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     5281 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     5341 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     5401 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     5461 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     5521 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     5581 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     5641 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     5701 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     5761 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     5821 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     5881 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
     5941 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     6001 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     6061 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     6121 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     6181 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     6241 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     6301 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     6361 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     6421 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     6481 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     6541 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     6601 tacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     6661 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     6721 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     6781 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     6841 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     6901 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     6961 atgaccccga agcagggtta tgcagcggaa aagatccgtc gaccctttcc gacgctcacc
     7021 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     7081 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
     7141 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     7201 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     7261 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     7321 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     7381 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     7441 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     7501 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     7561 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     7621 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     7681 ggccgcgaac ggcctcaccc caaaaatggc agccaagctg accacttctg cgctcggccc
     7741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8881 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8941 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9001 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9061 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctagcttccc
     9121 gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac ggtgcgatgg gtcccggccc
     9181 cggtcaagac gatgccaata cgttgcgaca ctacgccttg gcacttttag aattgcctta
     9241 tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc gtccaatcaa agcctagaaa
     9301 atataggcga agggacgcta ataagtcttt cataagaccg cgcaaatcta aaaatatcct
     9361 tagattcacg atgcggcact tcggatgact tccgagcgag cctggaacct cagaaaaacg
     9421 tctgagagat accgcggatc tcacacagga aatttc
//
