LOCUS       sequence-316562-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018325
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2163..2183
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2184..2207
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2233..2289
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2290..2307)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2290..2306)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2299..2321)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2515..2534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2634..2656
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2694..2712)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3498..4697
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4928..6076)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6348..6719)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6752..6861
                     /note="incP origin of transfer"
     rep_origin      7014..7645
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8108..8696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8597..8616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8850..8867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8984..9005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9020..9025,9026..9043,9044..9050)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9058..9074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9063..9085
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9082..9098
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9082..9098
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcgca
      361 aggtactgct ggcgacctat ctcggctcag ccgccgcgct cgccgtcggc tccgcctcgg
      421 cgaacgacga gctgatcaag atgtcgcaga acccgaagga ctgggtgatg ccggcgggtg
      481 actacgcgaa cacccgctac tccaagctca accagatcaa cgcccagaac gttgggaaac
      541 tgcaggtcgc ctggaccttc tccacggggg tcctccgggg ccatgaaggc ggccccctga
      601 tcatcggcaa tatgatgtac gttcataccc cgttcccgaa taaggtctat gccatcgacc
      661 tgtccaacga gaataagatc gtctggaagt atgaacctaa gcaggacccg aatgtcatcc
      721 ccgtgatgtg ctgcgacacc gtgaaccgcg ggctgagcta tggggatggc aagatcatct
      781 tgcaccaagc cgacacgaac ctcgtcgccc tggacgcgaa gaccggacag gtcgcgtggt
      841 ccgcgaccaa cggcgacccg tccaagggcc agacgggcac gtccgcggcc ctcgtcgtga
      901 aggacaaggt actggtcggc atctcgggcg gcgagttcgg agtgcagtgc cacgtgaccg
      961 cgtacgacct caagagcggg aagcaagtgt ggcgcgcctt ttccgaaggc cccgatgacc
     1021 aaatcaaagt ggaccccgcc aagaccacct cgctcgggaa gcccgtgggc gccgactcgt
     1081 cgctcaagac gtggcagggt gaccagtgga agatcggggg cggctgcacc tggggctgga
     1141 tgtcgtacga ccccgcgctc aacctcgttt actacggctc cggcaacccc tcgacgtgga
     1201 acccgaagca gcggcccggg gataacaaat ggtcgatgac catcttcgcg cgggatgccg
     1261 acacgggcat ggcgaagtgg gtctaccaaa tgacacccca cgacgagtgg gactatgacg
     1321 gagtcaacga gatgatcctt tccgaccagc agatcaacgg ccaagcccgg aagctgctga
     1381 cccacttcga tcggaacggc ctcggctaca ccatggaccg ggagagcggc gagctcctcg
     1441 tggcggagaa gtatgacccg aaggtgaact ggacatcggg cgtggatatg gataagaact
     1501 cccccaccta tgggcgcccc aaggtcctcg acgcggcttc gaccgacaag gcgggcgagg
     1561 accataacgt gaagggcatc tgccccgccg ccctcggcac caaggatgag caaccggcag
     1621 cctatagccc ggacacccag ttgttctatg tgcccacgaa ccacgtctgc atggactacg
     1681 agccgttcaa ggtcagctac accgccggac agccgtacgt cggcgcgacc ctgtcgatgt
     1741 acccgccgca gggcgaaagc cacatgggca acttcatcgc ctgggacggc aagaccggca
     1801 agatcgtctg gtctaacaag gaacagttca gcgtctggag cggggccttg gctaccgcgg
     1861 gtggcgtcgt cttctatggc accctcgagg gctacctcaa agcggtggat gcgaagtccg
     1921 ggaaggagct gtacaagttc aagacgccct ccggcatcat tgggaacgtc acgacctacg
     1981 agaacggcgg caagcagtat gtcgccgtgc tctcgggcgt gggcggttgg gccggcatcg
     2041 gtctcgccgc gggccttaca gacccgactg ccgggctcgg cgcggtcggc ggctacgcgg
     2101 ccctgagcaa ctacacggcg ctcggtggga ccctgaccgt cttctccctc ccggccaacg
     2161 ccgaaaacct ctattttcag ggacatcatc accatcacca tcaccactga cagctatgac
     2221 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     2281 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2341 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2401 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2461 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2521 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2581 ctgacgggct tgtatgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2641 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2701 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagc accctttctc
     2761 ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg
     2821 agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc
     2881 aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg
     2941 ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg
     3001 acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt
     3061 tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg
     3121 agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg
     3181 gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc
     3241 agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc
     3301 gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca
     3361 cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata
     3421 atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc
     3481 tggtccggag gccagacgtg aaacccaaca tacccctgat cgtaattctg agcactgtcg
     3541 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
     3601 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg
     3661 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa
     3721 tcttgctcgt ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt
     3781 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
     3841 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
     3901 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
     3961 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
     4021 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
     4081 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     4141 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     4201 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     4261 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     4321 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     4381 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     4441 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     4501 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     4561 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     4621 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc
     4681 aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt
     4741 ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat cggcagttcg
     4801 tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg
     4861 cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca
     4921 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     4981 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     5041 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     5101 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     5161 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     5221 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     5281 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     5341 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     5401 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     5461 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     5521 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     5581 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     5641 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     5701 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     5761 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     5821 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     5881 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
     5941 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     6001 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     6061 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
     6121 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
     6181 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
     6241 tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
     6301 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
     6361 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
     6421 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
     6481 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
     6541 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
     6601 gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
     6661 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     6721 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     6781 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     6841 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     6901 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     6961 ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt tccgacgctc
     7021 accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca
     7081 gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg
     7141 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
     7201 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
     7261 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
     7321 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
     7381 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
     7441 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
     7501 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
     7561 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
     7621 ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc
     7681 ctcggccgcg aacggcctca ccccaaaaat ggcagccaag ctgaccactt ctgcgctcgg
     7741 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7801 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7861 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7921 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7981 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8041 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8101 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8161 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8221 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8281 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8341 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8401 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8461 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8521 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8581 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8641 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8701 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8761 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8821 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8881 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8941 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9001 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9061 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctagctt
     9121 cccgcttggt cgggccgctt cgcgagggcc cgttgacgac aacggtgcga tgggtcccgg
     9181 ccccggtcaa gacgatgcca atacgttgcg acactacgcc ttggcacttt tagaattgcc
     9241 ttatcgtcct gataagaaat gtccgaccag ctaaagacat cgcgtccaat caaagcctag
     9301 aaaatatagg cgaagggacg ctaataagtc tttcataaga ccgcgcaaat ctaaaaatat
     9361 ccttagattc acgatgcggc acttcggatg acttccgagc gagcctggaa cctcagaaaa
     9421 acgtctgaga gataccgcgg atctcacaca ggaaatttc
//
