LOCUS       sequence-31657-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM
  TITLE     Engineered allosteric activation of kinases in living cells.
  JOURNAL   Nat Biotechnol. 2010 Jul;28(7):743-7. doi: 10.1038/nbt.1639. Epub 
            2010 Jun 27.
  PUBMED    20581846
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1639"; 
            journalName: "Nat Biotechnol"; date: "2010-07"; volume: "28"; issue:
            "7"; pages: "743-7"
COMMENT     Sequence Label: myc-Rapr-FAK-YM
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             190..219
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc tgcagatatc catcacactg gcggccccct ctaggtcgac gattgaattg
      181 gccaccatgg agcagaagct gatctccgag gaggacctgg gatcccctgc ttatcttgac
      241 ccaaacttga atcacacacc aagttcgagt actaagactc acctgggtac tggcatggaa
      301 cggtcccctg gtgcaatgga acgagtatta aaggtctttc atcattttga aagcagtagt
      361 gagccaacca cctgggccag tattatcagg catggagatg ctactgatgt caggggcatc
      421 attcagaaga tagtggacag tcacaaagta aagcacgtgg cctgctatgg atttcgcctc
      481 agtcacctgc gatcggagga ggtgcactgg ctgcacgtgg acatgggtgt ctccagtgtg
      541 agggagaaat atgaactcgc ccacccacca gaggagtgga aatatgagtt gagaattcgt
      601 tacttgccaa aaggatttct aaaccagttt actgaagata agccaacatt gaatttcttc
      661 tatcaacagg tgaagagtga ctacatgcaa gaaatagctg atcaagtaga ccaagaaata
      721 gctttgaagt tgggttgttt ggaaattagg cgatccgctt gggaggcgag gggtaatgcc
      781 ctagagaaga agtccaacta tgaagtatta gaaaaagatg ttggtttaaa gcgatttttt
      841 cctaagagtt tactgg
//
