LOCUS       sequence-316574-        9462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018331
FEATURES             Location/Qualifiers
     source          1..9462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2166..2186
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2187..2210
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2236..2292
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2293..2310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2293..2309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2302..2324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2518..2537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2637..2659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2697..2715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3501..4700
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4931..6079)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6351..6722)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6755..6864
                     /note="incP origin of transfer"
     rep_origin      7017..7648
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8111..8699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8600..8619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8853..8870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8987..9008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9023..9028,9029..9046,9047..9053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9061..9077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9066..9088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9085..9101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9085..9101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcggc
      361 tcgatctgat ctttgcgggc tgcatctccg ccctcgccgg ggccatgatc atcgccggct
      421 cggcagtcgc ggacccggtg gacgatgcgc ggctccgctc cgccgaccag gacacggtca
      481 actgggtgct gccggggcgc gactacggga accgccgttt cagcccgctc gaccgcatca
      541 ccccggagac ggcgaagcgg ctggtgccca agtggatcta ccagaccggc tacgcggcga
      601 cgttccagac cagcccactc gtggccgacg gcgtgatgta tctgtccgcc ccgttctccc
      661 atgtcatggc catcgacgcg aagaccgggc gcgagatctg gcgatatgag cacaagtcga
      721 ccgcgaagaa gctgtgctgc ggcccggcca accgcggcgt ggctctcgga tacggcctgg
      781 tcttcgtggc gtcggtcgac gcccggctgg tcgcgctgga ccagaagacc ggcaagccgg
      841 tgtgggacaa gccgctggtg gatgccgacg acgtgttgac gtccgaggac gccaagtcgc
      901 tcggggctga cgacccggtc cttaagggac aggtctccgg cgcgacggga gtgggctcca
      961 acagcgcgcc cctggtctat gatggcaagg tgttcgccgg aatcacgggc gtggggtatg
     1021 ggctccacct cgacagctcg cggcctggcg cccctctggg cgccgtgatc ggcgtcgccg
     1081 gccggtacgg ccgtccgggc ttcctcgcgg cctacgactc gaagaccggc gaccaggtgt
     1141 ggcggttcga caccacgcgc ccggattggg agggggagta tcgggagacg acggcgtatg
     1201 gcgtcccgat gcatcgcgat gtcgcggccg aaaaggccgc ggagccggct cataaggatg
     1261 cctggaagtt cggcggcggc agcatctggc atgcgcccgc cctggatccg gagcggggcc
     1321 tgctgttctt cggcgtgggc aacccctcgc cgcaggcggc cggcgaatcg cgtccaggag
     1381 acaacctgta cacctcagct ctggtggcgc tcgacgccaa gacgggcaaa atccggtggt
     1441 ccttccagca gacgccgcac gacctctggg gctacgacgt ggcgtcgccc ccttccctct
     1501 tcgacttggt ggtcgacggg aagaagcgcg cggtggtcgg gcaggcctcc aagctcggct
     1561 ggtacttcgt ccacgaccgc gagaccggcg agctcttgtt caagagcgac ccgtatgttc
     1621 cgcagtcaaa tctcttccag ccgccgagca aggagggggt cgtgatcgct ccgggcgtcg
     1681 gcgggggcgt gaactggtcg cccgcctcga tcgatgaggc gaagggcgtc gcctacgtcg
     1741 cggcgatgca ctggccggcg acctaccgcg cccagaccct gccggcggcc gacggcaagc
     1801 cggaggtccg ctacttcaat atagagcccg cgaccagcgg gacctacggc ctgctctccg
     1861 ccatcgacct tgcgggcggt ggccgcatta agtggcaggc gaagaccccg gagccgctga
     1921 tcggtggtgt tctcgccgcg aagggcggtg tcgtattcac cggggagggc aacggcgact
     1981 tctccgcgtt cgacgccgag cggggtgata aggtgtggtc gttcaacacg ggcgcggggg
     2041 tcaacgcccc gcccatcgcc tactcgctcg agggcaagga ctatgtcgcg gtagccgcgg
     2101 gcggctcgca gatctggggc ttccgacagg ggggcgccgt catcgccttc ggcctcccgg
     2161 acgccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagctat
     2221 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     2281 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2341 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2401 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2461 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2521 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2581 gccctgacgg gcttgtatgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2641 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2701 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
     2761 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     2821 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     2881 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     2941 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     3001 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     3061 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     3121 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     3181 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     3241 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     3301 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     3361 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     3421 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     3481 ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg
     3541 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     3601 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     3661 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     3721 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     3781 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     3841 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     3901 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     3961 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     4021 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     4081 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     4141 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     4201 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     4261 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     4321 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     4381 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     4441 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     4501 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     4561 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     4621 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     4681 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     4741 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     4801 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     4861 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     4921 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     4981 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     5041 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     5101 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     5161 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     5221 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     5281 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     5341 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     5401 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     5461 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     5521 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     5581 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     5641 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     5701 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     5761 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     5821 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     5881 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     5941 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     6001 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     6061 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     6121 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     6181 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     6241 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     6301 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     6361 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     6421 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     6481 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     6541 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     6601 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     6661 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     6721 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     6781 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     6841 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     6901 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     6961 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg
     7021 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     7081 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     7141 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     7201 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     7261 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     7321 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     7381 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     7441 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     7501 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     7561 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     7621 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     7681 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc aagctgacca cttctgcgct
     7741 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7801 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7861 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7921 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8761 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8821 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8881 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8941 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9001 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9061 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag
     9121 cttcccgctt ggtcgggccg cttcgcgagg gcccgttgac gacaacggtg cgatgggtcc
     9181 cggccccggt caagacgatg ccaatacgtt gcgacactac gccttggcac ttttagaatt
     9241 gccttatcgt cctgataaga aatgtccgac cagctaaaga catcgcgtcc aatcaaagcc
     9301 tagaaaatat aggcgaaggg acgctaataa gtctttcata agaccgcgca aatctaaaaa
     9361 tatccttaga ttcacgatgc ggcacttcgg atgacttccg agcgagcctg gaacctcaga
     9421 aaaacgtctg agagataccg cggatctcac acaggaaatt tc
//
