LOCUS       sequence-316575-        9471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018333
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2175..2195
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2196..2219
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2245..2301
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2302..2319)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2302..2318)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2311..2333)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2527..2546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2646..2668
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2706..2724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3510..4709
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4940..6088)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6360..6731)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6764..6873
                     /note="incP origin of transfer"
     rep_origin      7026..7657
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8120..8708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8609..8628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8862..8879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8996..9017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9032..9037,9038..9055,9056..9062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9070..9086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9075..9097
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9094..9110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9094..9110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accttgggat
      361 ggttcggcct cgaacgtatg cagggccgga agccgatgaa caagatcatt atggccgccg
      421 tcgccggcct cgtcgcgacc gcgggctggc actcggcggc cctcgccgcc ggcgtcactg
      481 aggaggatct ggcgaacgat acggcaaaca cctcctcagt gctcaccaac ggcatgggcc
      541 ggcatctgca gcggtacagc ccgctcgata tcctcaacaa ggataacgtt aagaacctca
      601 tgccggcctg ggcgttctcc ctgggcgggg agaagcagcg cggccaggag acccagccgc
      661 tgatccatga cggcgtgatg tatatcacag ggtcgtacag ccgcatgtac gctatcgatg
      721 tcaacaccgg cgaggagctg tggcagtacg acgcccgcct cccggagggc atcctcccgt
      781 gctgtgacgt catcaaccgg gggggcgcga tctacggcga caagatctac ttcggaaccc
      841 tcgacgccag gatcgtcgcg ctcgacctca agacggggga cgtcgtctgg aacaaaaaga
      901 tcgccgacta caaggcgggc tactcgtaca cggcagcccc gctcatcgtg aaggggctcg
      961 tgatcaccgg gaactcgggt ggcgaattcg gcatcgtcgg cgaagtccag gcgcgcgacg
     1021 ccgagaccgg cgaaatggtt tggacccggc ccgtgatcga gggacacatg gggacgttca
     1081 agggcgagga atcgaccatg accggcaccc tgaacgccac gtggccggga gacatgtgga
     1141 agacgggagg cggggccacc tggctgggcg ggtcgtacga cgcggagacg gacaccctcg
     1201 tcttcggcgc cgggaacccg gccccgtgga actcgtggct ccgggacgcg ggccaaaaca
     1261 acgatgggaa gggcgacaat ctttatgcgg cctcccgcat cggcatcaac cccgacaacg
     1321 gagagatcaa gtggcacttc cagaccacgc cgcgcgaggg ctgggattac gacggcgtca
     1381 atgaggtggt cgcctacact gacagggacg gcaataagcg ctgggcgacc gcggatcgaa
     1441 acggttactt ctacgtgctc aatcgtgagg atggcaagtt cgtgcgcggg gtcccgttcg
     1501 tcaaggacat cacttgggcc tcggggctgg atgagaacgg gcgcccgatc ttcaacgagg
     1561 acaaccgtcc cggggacccc gcagccgcgg cggacggcgc caagggcgag gtcatcttcg
     1621 cgagcccgtc gttcctcggc gggaagaact ggatgccgat ggcgttctcg cagaagaccg
     1681 gcaacttcta cgtcccgtcg aacgagtggg gtatggatat ctggaacgaa cccatcacct
     1741 ataagaaagg cgccgcctac ctcggcgccg gcttcacgat caagccgaat tacgaggacc
     1801 atatcggcag cctcaaggcc atcgacccgg acacaggcga gctgaagtgg gaattcaaga
     1861 acgatgcccc cttgtggggc ggggtcatga ccaccgccgg tggcctggtg ttcaccggga
     1921 ccccggaggg caagttcatc gctttcgacg atgagaccgg agagatcctc tggagcttcc
     1981 agacgggctc gggcatcgtg ggccagccgg tcacgtggga gatggacggc gaacagtacg
     2041 tgagcgtcat ctctggctgg ggcggggcgg tgcccctatg gggaggagag gtcgccaaga
     2101 aggtgaacta tctcaatcag ggcggcctcc tctggacgtt caagctcccc aagcagctgg
     2161 cggccgcgaa cgccgaaaac ctctattttc agggacatca tcaccatcac catcaccact
     2221 gacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
     2281 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2341 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2401 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     2461 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2521 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2581 cgctgacgcg ccctgacggg cttgtatgct cccggcatcc gcttacagac aagctgtgac
     2641 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     2701 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2761 gcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
     2821 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
     2881 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg
     2941 gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc
     3001 atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc
     3061 gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca
     3121 tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc
     3181 cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc
     3241 agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc
     3301 cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg
     3361 tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca
     3421 ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg
     3481 accagcggag gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc
     3541 tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc
     3601 tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc
     3661 tgtatgcgtt ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg
     3721 ggcggcggcc aatcttgctc gtctcgctgg ccggcgccac tgtcgactac gccatcatgg
     3781 cgacagcgcc tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg
     3841 cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc
     3901 ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg
     3961 gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg
     4021 gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc
     4081 cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg
     4141 tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg
     4201 cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt
     4261 cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag
     4321 ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct
     4381 acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg
     4441 cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg
     4501 aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg
     4561 gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat
     4621 ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga
     4681 gcggcgcagg gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg
     4741 tcaaggcgac ttccggcaag ctatacgcgc cctagaattg tcaattttaa tcctctgttt
     4801 atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc
     4861 gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg
     4921 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt
     4981 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact
     5041 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt
     5101 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc
     5161 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct
     5221 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt
     5281 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg
     5341 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc
     5401 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct
     5461 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc
     5521 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct
     5581 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca
     5641 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga
     5701 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca
     5761 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca
     5821 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg
     5881 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag
     5941 cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc
     6001 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt
     6061 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg
     6121 ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat
     6181 ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg
     6241 tattccgaat cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc
     6301 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
     6361 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
     6421 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
     6481 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
     6541 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
     6601 gccagcaggt aggcctacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
     6661 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt
     6721 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
     6781 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
     6841 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
     6901 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
     6961 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagat ccgtcgaccc
     7021 tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct
     7081 gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt
     7141 ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga
     7201 ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg
     7261 acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca
     7321 cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg
     7381 actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga
     7441 ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag
     7501 ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata
     7561 tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag
     7621 gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca
     7681 ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcca agctgaccac
     7741 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7801 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7861 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7921 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8761 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8821 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8881 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9001 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9061 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9121 ccaagctagc ttcccgcttg gtcgggccgc ttcgcgaggg cccgttgacg acaacggtgc
     9181 gatgggtccc ggccccggtc aagacgatgc caatacgttg cgacactacg ccttggcact
     9241 tttagaattg ccttatcgtc ctgataagaa atgtccgacc agctaaagac atcgcgtcca
     9301 atcaaagcct agaaaatata ggcgaaggga cgctaataag tctttcataa gaccgcgcaa
     9361 atctaaaaat atccttagat tcacgatgcg gcacttcgga tgacttccga gcgagcctgg
     9421 aacctcagaa aaacgtctga gagataccgc ggatctcaca caggaaattt c
//
