LOCUS       sequence-316577-        9474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018337
FEATURES             Location/Qualifiers
     source          1..9474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2178..2198
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2199..2222
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2248..2304
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2305..2322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2305..2321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2530..2549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2649..2671
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2709..2727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3513..4712
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4943..6091)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6363..6734)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6767..6876
                     /note="incP origin of transfer"
     rep_origin      7029..7660
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8123..8711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8612..8631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8865..8882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8999..9020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9035..9040,9041..9058,9059..9065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9073..9089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9078..9100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9097..9113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9097..9113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgctcc
      361 ttgccctggc ggtgggcacg gcgaccgtca tcccgtcggc ctactcgaac gacgacgtcc
      421 tcaagctcac cgagaacccg aagaactggg cggcccccgg caaggattac gccaacacgc
      481 gccactcgtc cctcaagcag atcaataccc agaacgtcaa gagtctccac atggcctggt
      541 cattctcgac ggggacgctt cgcggccacg agggccagcc cctggtcgtc ggcgatcgga
      601 tgtacgtggt gagcagctac ccgaacatcg tctgggcgct cgacctgtcc aagggtggct
      661 cgtatgaggt gctctggaag tatgtccccc gccaagatga taaggccgtc agcaccgcat
      721 gctgcgacac ggtgaaccgc ggcgcgtcct acgccgatgg caaggtcgtc ttcaacacgc
      781 tggacggcta cgtggtgtgc ctggatgcga acaccggcaa ggagctctgg aagacgaagt
      841 tcgccgacct caacaagggc gagaccagca cccccgcgcc catcatcgtc aaagacaagg
      901 tcttcaccgg ctacggcggt gatgagttcg gggcgcgggg ccgcttcgcc gccttcgacc
      961 taaacagcgg caaaatggtc tggcaggcgt atagcaacgg cccggactcg gatgtccttc
     1021 tcgggcccga cttcaactcg aaacatccgg agtacggcca ggccggccag gatctcggcg
     1081 tgaagaccta cccggacgag gagtggaaac ggggcggtgg ctgcgcgtgg ggctggtact
     1141 cgtacgaccc gaagctcgac ctggtgtact acaacacggg caacccgggg ctgtggagcc
     1201 cgtcgtaccg cacagaggcg aagacccacg aggaagccaa cgagccatgg aagtgggaca
     1261 ataagtggtc catgaccgtc ttcgcccgga agccagatac cggcgaagcg gtctggggct
     1321 accagctcac ccccttcgac cagtgggact acgatgccat caacgaggac gtcctggtcg
     1381 acatcaccgt cgacggcgcg aagaagcctt gcctcgtcca cttcgaccgg aacggcttcg
     1441 cctacgtgct ctcgcgcacg gatgggaccc tcatccgggc caacaaattc gtcaccgtca
     1501 actgggcgga aaaaatcgat atgaagaccg gccggccggt caaggtgaaa gagcactcgc
     1561 cgttcgaggt tggaaaggcg gtccaggcgt accctagcgc ccagggcggc aaggatcaac
     1621 agccggtcgc ggtcgatccg aaggaggcca acgtgttcta cgcccccacc aacaactggg
     1681 gcatgaccct ggagccgatg gagcgcgccc acacgaacca aggttcggtc tacgtgttcg
     1741 cgaatgtgct gatgaagccc gagaagcccg gcgtaatggg ccggttcaag gccttcgatg
     1801 tcatcacggg gaaggcccgt tgggacatcc cggagcggtt cccgacctgg tccggggccc
     1861 tggtcacgga tggcggactg gcgttctacg gcacgctgga cggctggttc cgcgccgtcg
     1921 atcgcaagac cggcaaggtg ctctggcagc agaagctcgg ctcgggcatc atcggcaacc
     1981 ccatctcgtt cgaggtgggc ggcaagcagt atatctcgat cctcacgggc atcggcggct
     2041 ggatcggcct tcccgtcacg gccggccttg acccagccga cccgtacggc gcgctgggcg
     2101 tggcgggcat ggccggcgag aacggcttct acaacatccc gatgggaggc acgctctaca
     2161 cattctgcgt ccaagccgaa aacctctatt ttcagggaca tcatcaccat caccatcacc
     2221 actgacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga
     2281 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2341 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2401 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2461 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     2521 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     2581 acccgctgac gcgccctgac gggcttgtat gctcccggca tccgcttaca gacaagctgt
     2641 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2701 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2761 ttagcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca
     2821 tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg
     2881 tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg
     2941 ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt
     3001 gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc
     3061 tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg
     3121 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
     3181 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
     3241 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
     3301 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
     3361 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
     3421 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
     3481 cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacataccc ctgatcgtaa
     3541 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
     3601 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
     3661 cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
     3721 tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cactgtcgac tacgccatca
     3781 tggcgacagc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     3841 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     3901 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     3961 tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     4021 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     4081 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     4141 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     4201 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     4261 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     4321 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     4381 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     4441 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg
     4501 aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg
     4561 tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg
     4621 catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt
     4681 ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggcc tatgccatgc
     4741 gggtcaaggc gacttccggc aagctatacg cgccctagaa ttgtcaattt taatcctctg
     4801 tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc
     4861 gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag
     4921 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
     4981 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
     5041 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
     5101 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
     5161 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
     5221 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
     5281 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
     5341 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
     5401 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
     5461 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
     5521 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
     5581 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
     5641 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
     5701 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
     5761 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
     5821 tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg
     5881 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
     5941 tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
     6001 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
     6061 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
     6121 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
     6181 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
     6241 tggtattccg aatcttgccc tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg
     6301 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
     6361 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
     6421 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
     6481 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
     6541 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     6601 acggccagca ggtaggccta caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     6661 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     6721 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     6781 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     6841 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     6901 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     6961 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gatccgtcga
     7021 ccctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     7081 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     7141 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     7201 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     7261 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     7321 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     7381 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     7441 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     7501 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     7561 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     7621 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
     7681 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag ccaagctgac
     7741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9121 acgccaagct agcttcccgc ttggtcgggc cgcttcgcga gggcccgttg acgacaacgg
     9181 tgcgatgggt cccggccccg gtcaagacga tgccaatacg ttgcgacact acgccttggc
     9241 acttttagaa ttgccttatc gtcctgataa gaaatgtccg accagctaaa gacatcgcgt
     9301 ccaatcaaag cctagaaaat ataggcgaag ggacgctaat aagtctttca taagaccgcg
     9361 caaatctaaa aatatcctta gattcacgat gcggcacttc ggatgacttc cgagcgagcc
     9421 tggaacctca gaaaaacgtc tgagagatac cgcggatctc acacaggaaa tttc
//
