LOCUS       sequence-316583-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018345
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2193..2213
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2214..2237
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2263..2319
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2320..2337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2320..2336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2329..2351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2545..2564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2664..2686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2724..2742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3528..4727
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4958..6106)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6378..6749)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6782..6891
                     /note="incP origin of transfer"
     rep_origin      7044..7675
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8138..8726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8627..8646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8880..8897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9014..9035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9050..9055,9056..9073,9074..9080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9093..9115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9112..9128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9112..9128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcggc
      361 cgtcggtggc gatcgccgcg atgctcatct ggtgctgtgc ccccgcgctc gcccaacagg
      421 agggggcagc cctgtatgcg tcccactgcg cccagtgcca tgacgggggt gacgcccaga
      481 gccgggtccc cggacgctcc gccatgcagg gcatgtcctt cgaccacgtg ctcggcaccc
      541 tcacgaccgg ctcgatggcg gcgatggcca aggaacggtc cgatgaggag cgccgcgcga
      601 tcgcctcgtt cgtgacgggc aagacagcca cggggggcgt ggcggcggat gcggagggcc
      661 ggtgcaccca acaggtgcgg ggcttcccgg agcccctcga cggccctcgc tggaacgggt
      721 ggggcgtgga tgcgggcaac tcgcgcttcc aaccggccga tatggcgggc ttgaccgccg
      781 aggaggtgcc ccgcctccgg ctcaagtggg cctacgcgtt cccgggggcc tcggtgtcat
      841 tcgcgcctcc caccatcatg ggcgggctcc tgttcatcgg ggggaccgac cggaaggtgc
      901 acgcgctgga cgcccgctcg ggctgcacgc tctggacgtt cgcgacggat gcggcggtcc
      961 gcgccgccat ctccttcgcc ccgctccccg gcactgatca gttcgtgatc ttcttcgggg
     1021 atctcagggc caacgcgtac gcagtgaacg cgctcacggg ggccctcatc tggaagacga
     1081 aggtcgagga gcacccggcg gcgcgcatca cgggtgcccc gaccctgcac tcgggcgtgc
     1141 tgtatgtgcc cgtgtcgtcg ctggaggagg ccgcgggctc gcaggcgtcg tacgagtgct
     1201 gcaccttccg cggctcggtc gtggccctgg aggccgtcac cggtaagccg gtgtggcagg
     1261 cgtacaccat cccggaagtc ccgcacccca ccggcaagaa cacgaagggc acccagctct
     1321 tcgggccgtc gggcgcggcc gtgtggtcgg cgccgaccgt cgacccgaag cggaacgccg
     1381 tgtatgtggc gacctcgaat tcctactcga acccgccggc ggccacggcg gacgccattc
     1441 tggccttcga gctcgccacc ggcaagctcc tgtggaagca gcaggccacc ccgaaagatg
     1501 ccttcatcgt cgcctgctat ggcgccgacc ggacaaactg cccggacgag cacgggcccg
     1561 accacgactt cggccaatcc cccatcctcg tgaccctgcg tgatggccgg cgggtgctgg
     1621 ccatcgccca gaagtcgggc gtcgtccatg ccctggatcc ggacgacggg gggaagatcc
     1681 tgtggcagac ccgcatcggc aagggcggtg cgctgggcgg ctccgaatgg gggagcgccg
     1741 cggaccagga ccgcatctac gtggccaact cggacgtccg atttctgcat gacggcacgc
     1801 ggcgcctgga ctccacccag ggcggcggac tcttcggcct cgatctggcc tcgggcaaga
     1861 tcgcgatgga ggtgccgccg gtggcctgcg gcgaccggct gcagtgcagc cccgccctct
     1921 ccgcggccgt ctcgctgatt ccgggcgtgg tcttctccgg aggcgtgtcc gggttcctgc
     1981 gcgcctatgc caccgccgac ggcaagctcc tctgggagtt cgacacggcc cgggattaca
     2041 ccgccgtcaa tggcgtcccc gcgcacggcg gtgccatcga tggcccgggc ccggtgatcg
     2101 ccggcggtat gctgtacacc aacagcggct acggccagtg gagtggcgtg gcgggcaacg
     2161 tgctcctcgc gttcgaggtg ggcaaggagg ccgaaaacct ctattttcag ggacatcatc
     2221 accatcacca tcaccactga cagctatgac catgattacg ccaagcttgc atgcctgcag
     2281 gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     2341 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     2401 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     2461 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2521 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     2581 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtatgctcc cggcatccgc
     2641 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2701 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2761 gataataatg gtttcttagc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     2821 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
     2881 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     2941 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     3001 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     3061 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     3121 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     3181 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     3241 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     3301 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     3361 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     3421 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     3481 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     3541 tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     3601 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     3661 atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg
     3721 cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg
     3781 tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg
     3841 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     3901 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     3961 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
     4021 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     4081 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     4141 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     4201 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     4261 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     4321 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     4381 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     4441 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     4501 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     4561 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     4621 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     4681 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     4741 aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc tagaattgtc
     4801 aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact
     4861 gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg
     4921 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     4981 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     5041 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     5101 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     5161 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     5221 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     5281 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     5341 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     5401 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     5461 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     5521 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     5581 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     5641 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     5701 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
     5761 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     5821 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg
     5881 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
     5941 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
     6001 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
     6061 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
     6121 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
     6181 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
     6241 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc tccgcgaagt
     6301 cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt
     6361 tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg
     6421 ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg
     6481 aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc
     6541 aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt
     6601 ttgtagccct ggccgacggc cagcaggtag gcctacaggc tcatgccggc cgccgccgcc
     6661 ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc
     6721 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     6781 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     6841 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     6901 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     6961 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     7021 gaaaagatcc gtcgaccctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     7081 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     7141 cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
     7201 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     7261 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     7321 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     7381 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     7441 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     7501 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     7561 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     7621 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc
     7681 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat
     7741 ggcagccaag ctgaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7801 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7861 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7921 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7981 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8041 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8281 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8881 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8941 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9001 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9061 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9121 gctatgacca tgattacgcc aagctagctt cccgcttggt cgggccgctt cgcgagggcc
     9181 cgttgacgac aacggtgcga tgggtcccgg ccccggtcaa gacgatgcca atacgttgcg
     9241 acactacgcc ttggcacttt tagaattgcc ttatcgtcct gataagaaat gtccgaccag
     9301 ctaaagacat cgcgtccaat caaagcctag aaaatatagg cgaagggacg ctaataagtc
     9361 tttcataaga ccgcgcaaat ctaaaaatat ccttagattc acgatgcggc acttcggatg
     9421 acttccgagc gagcctggaa cctcagaaaa acgtctgaga gataccgcgg atctcacaca
     9481 ggaaatttc
//
