LOCUS       sequence-316586-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within 
            the N-terminal ankyrin repeat domain-1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, 
            Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG
  TITLE     Loss of Integrin-linked kinase sensitizes breast cancer to SRC 
            inhibitors.
  JOURNAL   Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373.
  PUBMED    34921014
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373."
COMMENT     Sequence Label: PX459v2-ILK-gRNA 1-exon 1
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        159..234
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
       61 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      121 ggaaaggacg aaacaccgcg gagaacgacc tcaaccaggt tttagagcta gaaatagcaa
      181 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      241 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
      301 attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtac
//
