LOCUS       sequence-316589-        8265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CckA FRET Sensor with DITE motif mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Duvall SW, Childers WS
  TITLE     Design of a Histidine Kinase FRET Sensor to Detect Complex Signal 
            Integration within Living Bacteria.
  JOURNAL   ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 
            10.1021/acssensors.0c00008. Epub 2020 Jun 16.
  PUBMED    32495620
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.0c00008"; journalName: "ACS Sens"; date: 
            "2020-06-26- 26"; volume: "5"; issue: "6"; pages: "1589-1596"
COMMENT     Sequence Label: pSWD95D293A
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1..110
                     /note="incP origin of transfer"
     primer_bind     262..278
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      279..325
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4006..4027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5627..6331
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIA
                     LKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAIDHRLERIEESDNETYVVQREVAVAK
                     YSNLGGGMDELYK"
     primer_bind     complement(6350..6366)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6377..6394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6377..6393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6386..6408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6591..7179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6671..6690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7505..8164
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7571..7590)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
       61 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
      121 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
      181 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
      241 aaaagatccg tcgacggccg ctctagaact agtggatcca aataaaacga aaggctcagt
      301 cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca ttatacgcaa ggcgacaagg
      361 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      421 gaatgcttaa tgaattacaa cagtttttat ataagcttag cgctaccgga ctcagatcca
      481 gatcgaaatg taccggctga actggtggaa gcacgttgag cctcgtcaag gactcatgaa
      541 gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag gtgtcaaaac accgtctgtt
      601 catttggcta gacgctagag ttcttccgga tcacaagctc caggtcgtta cactcgatga
      661 cgactgctca ttcggagttt tacactcaag gttccacgag gtgtgggctc ttgcagcggg
      721 gtcgtggcac ggctcaggca atgatccgcg ctacaccatc tccaccacct tcgagacctt
      781 ccccttcccg gaaggcctga cgcccaacat agccgccgtc gactatgagg gcgacccgcg
      841 cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg ctgcgggaag cctggctcaa
      901 tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc ggctatcccg accgcgtgct
      961 gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc accctgacca acctctacaa
     1021 ccagcgcccg gcctggctgg acatggccca ccagcgcctg gacgccgccg tcgccgccgc
     1081 ctacggctgg cccgacggcc tgaccgacga cgagatcctg gagcgcctgt tcgccctcaa
     1141 ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc caaaaccccg acctccccgg
     1201 cgaaggccgg ggtccagtta catccggaac gcttggagtg ggcccgccta cggcctcgac
     1261 caacccgcag tcggcaaggg gttcgatctg gaccccggca ttcgccgggg aggtcggcag
     1321 agaggtcaga accgcgtctt gcagccctgc ttcagatgaa ccccgcgcag gaagccgcgg
     1381 cgggccaggc cggcgtcgac ggcggcgttc atctggcggt cggcgggggc agcgccggtg
     1441 acttcgcgca ccaggccccg gaacgggatg atgctgttga tcagggtctt gccaccggcc
     1501 tcggccagct tgccggctcg gttttcggcg tactcggcgc cgaccttgta gtcgggcccc
     1561 agcgccgcgt tcagcgcctt cacctcattg gccagggcct ggcaggactt cagacccgcg
     1621 aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg gcacctcgcg cttggacagg
     1681 ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct tcttgcccgc ctcgccccag
     1741 cggccggacg gttcctcggg cttggccggc ggcttgacct gggcgtgggc gctggaggcc
     1801 agcagcacgg cggcggctcc ggccatgatc agcagcttgt tcggcatcaa aagcgccctc
     1861 ccgacaaaac acgccacggg aacggcaagc ctcgccgccg gttcccgccc gcaagtccct
     1921 cggcacgaac tctaaggcct gcgcacgatc catcgccacc acaaagcgtg tcatcccggc
     1981 cgaagcgcag cggagagccg ggacccaggg ccacaagcac ggcgcttcgt caccccctgg
     2041 gtcccggata gcgctccgcg cttccgggat gacacgggtt taagcacttg ggtgagaagc
     2101 cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc gtcgaaggcg tcgtcgccag
     2161 gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag cgatgaccag atgaaggcca
     2221 tctgctccat gatcgtcggg acgctggccg gatcgaagcc ggggtagagg tcttccacca
     2281 gccacaggcc cgtgccggga tcgaaggcga agcggcagcc gatcaggcgg aactgggcgc
     2341 gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc gtccaggtcg tcgtggtcca
     2401 gcaccaggca gcggatctgc agccagccgt ggtcgggcag caggtagaag gcgccctcgt
     2461 cctgatcctc gcccgaaacc tccagccccc ggtcgatggc ttcgacgaca tagccggccg
     2521 cgcggcaggt gtcggtgagc gcggccagca gggcggcttc ctggtcaggg gtcaggtcgg
     2581 tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt ctagcatgga ccgcccgcgc
     2641 ccgtgaggcc gaggatttcg cgctggtcag acaacctact tgccgtcccc acatgttagc
     2701 gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg gcgcttcaga cgctcgagtt
     2761 ttggggagac gaccatatgg ccgacttgca gctccaggac aaggtttcga ccggcgcccc
     2821 gcgtcggcgg tttgatccat ggctggtcgg cgcggcggtg tttttcgtgg ctgcggcggc
     2881 cctctcggcg gcgccggcgc tcaaggccgg accgaccacc ctggccggcc tgctgctgct
     2941 tctgggcgtc gcgggcgtgg ctgtgttggg ccttgtcgcc attcgcggct cagcgctttc
     3001 cggcggcgac gccgaccagg ctgaggggtt catcgaggcg ctggccgagc cggccgccct
     3061 ggccgccgcc gacggtcgcg tcctggccgc caacgggccc tggcgcgaag tcatgggcga
     3121 acagcgccgc ctgcccaagg gcgtggcggg ctccagcctg tttgcggccc tggtccaggc
     3181 gcgccagggg cagatggccg agggcatgct gagcgctgga ggaaccgact ataccgccaa
     3241 ggtctcgcgc cttgcgggcg gacggctgat gatccggctt gcgcccatcg ttgtcgctga
     3301 gccggttgtg gaagacgcgt cgtcgaacgt tacgcgtcac cggtcggcca ccgagatgag
     3361 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3421 aaacggccac aagttcagcg tccgcggcga gggcgagggc gatgccacca acggcaagct
     3481 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3541 caccttcggc tacggcgtgg cctgcttcag ccgctacccc gaccacatga agcagcacga
     3601 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct ctttcaagga
     3661 cgacggtacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3721 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3781 gtacaacttc aacagccact acgtctatat cacggccgac aagcagaaga actgcatcaa
     3841 ggctaacttc aagatccgcc acaacgttga ggacggcagc gtgcagctcg ccgaccacta
     3901 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3961 ccatcagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4021 gttcgtgacc gccgccctgg aaagcggcgg cgaagatccg atggtgagcc cggccccggt
     4081 cgccgagcgc gccgccccgc cacccagctc gctggacgcc ttcgccggag cctcgccgtt
     4141 cggcgcggcc ctgctggaag gcctggagcc gttcacctcg cgggtgctgg agaccaatcc
     4201 ggcgctgacc acgatgaccg gcgccaaggc cggtgtgctg ttcggcgatc tgatcgacgc
     4261 cgcctcgcgc gccgaggccg agacgcgcct gaacgaaggc cgcgccggtc cctacgaggt
     4321 gcggttggcg cgcgatccgt cgcgcatcgc tcacctttac ctctatcgcg ccgaaggccg
     4381 cctggtggcc tacatgatcg ccgtgtccga gcagaagcag atcgagctgc agctgtccca
     4441 ggcccagaag atgcaggcca tcggccagct ggccggcggc gtcgcgcacg acttcaacaa
     4501 cctcttgacc gccatccagc tgcgtctgga cgagttgctg catcgccatc ccgtcggtga
     4561 tccgtcgtac gagggtctca acgagatccg tcagacgggc gtgcgcgccg ccgacctcgt
     4621 gcgcaagctc ttggctttct cgcgcaagca gaccgtgcag cgcgaggtgc tggatctggg
     4681 cgagctgatc agcgagttcg aggtcttgct gcgccgcctg ctgcgcgaag acgtcaagct
     4741 gatcaccgac tatggccgcg acctgccgca ggtgcgcgcc gacaagagcc agctcgagac
     4801 ggcggtcatg aacctggccg tcaacgcccg cgacgccgtg cgcgcggcca agggcggcgg
     4861 cgtcgtgcgc atccgcaccg cgcgcctgac ccgcgacgag gcgatccagc tgggtttccc
     4921 ggccgccgac ggcgacacgg ccttcattga ggtcagtgac gatggtccgg gcattccgcc
     4981 cgacgtcatg ggcaagatct tcgacccgtt cttcaccacc aagccggtgg gcgagggtac
     5041 gggcctaggc ctagccacgg tctatggcat cgttaagcag agcgacggct ggattcacgt
     5101 ccacagccgt ccgaacgaag gcgcggcctt ccgcatcttc ctgccggtct atgaagcgcc
     5161 cgccggcgcg gtcgccgtcc aggccgtcgc cgagcccgcc aagccgcgcg ccgctcgcga
     5221 cctgtcgggc gccggccgca tcctgttcgt cgaggacgag gacgccgtgc gcagcgtcgc
     5281 cgcccgcctg ctgcgcgccc gtggctacga ggtgcttgag gcggccgacg gcgaagaggc
     5341 cctgatcatc gccgaggaaa acgccggcac gatcgacctc ttgatcagcg acgtgatcat
     5401 gcccggcatc gacggcccga ccttgctgaa gaaggcgcgt ggctatctgg ggaccgcgcc
     5461 ggtgatgttc atctccggct atgccgaggc cgagttcagc gacctcttgg aaggcgagac
     5521 gggcgtgacc ttcctcccca agccgatcga catcaagacc ctggccgagc gcgtcaagca
     5581 gcagctgcag gcggcgtcga acgttacgcg tcaccggtcg gccacctcta agggcgaaga
     5641 gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca
     5701 attcaaatgc acaggtgaag gagaaggcag accgtacgag ggagtgcaaa ccatgaggat
     5761 caaagtcatc gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat
     5821 gtatggcagc cgtaccttta tcaagtaccc ggccgacatc cctgatttct ttaaacagtc
     5881 ctttcctgag ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac
     5941 cgtcacgcag gacaccagcc ttgaggatgg cgagctcgtc tacaacgtca aggtcagagg
     6001 ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa
     6061 tacagagatg atgtatccag cagatggtgg tctgagagga tacactgaca tcgcactgaa
     6121 agttgatggt ggtggccatc tgcactgcaa cttcgtgaca acttacaggt caaaaaagac
     6181 cgtcgggaac atcaagatgc ccggtgtcca tgccattgat caccgcctgg aaaggatcga
     6241 ggagagtgac aatgaaacct acgtagtgca aagagaagtg gcagttgcca aatacagcaa
     6301 ccttggtggt ggcatggacg agctgtacaa gtaaagctgc agcccggggg atccactagt
     6361 tctagagcgg ccattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6421 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagcggtatc agctcactca
     6481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6841 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7021 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catcgaaccc
     7261 cagagtcccg gctagatcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     7321 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgggtaca gtctatgcct
     7381 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     7441 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gaggaggtaa
     7501 cgtcatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     7561 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     7621 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     7681 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     7741 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     7801 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     7861 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     7921 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     7981 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     8041 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     8101 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     8161 gtaacctagg catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     8221 cacaacgtgg ctttcccccc cccccccacc gcggtggcgg ccgcc
//
