LOCUS       sequence-316594-         482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within 
            the C-terminal protein kinase domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, 
            Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG
  TITLE     Loss of Integrin-linked kinase sensitizes breast cancer to SRC 
            inhibitors.
  JOURNAL   Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373.
  PUBMED    34921014
REFERENCE   2  (bases 1 to 482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373."
COMMENT     Sequence Label: PX459v2-ILK-gRNA 2-exon 8
FEATURES             Location/Qualifiers
     source          1..482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        153..228
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
       61 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      121 gacgaaacac cgacattgta gagggatcca tagttttaga gctagaaata gcaagttaaa
      181 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      241 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      301 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 ta
//
